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  • Product Name
    Phospho-ERK8 (Thr175+Tyr177) Antibody
  • Catalog No.
    AF0029
  • RRID
    AB_2833512
  • Source
    Rabbit
  • Application
    WB,IF/ICC,ELISA
  • Reactivity
    Human, Mouse
  • Prediction
    Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
  • UniProt
  • Mol.Wt
    60kD;
    60kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

ERK-7; ERK-8; ERK7; ERK8; Extracellular regulated kinase 8 delta; Extracellular signal regulated kinase 8; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8; MAP kinase 15; MAPK 15; Mapk15; Mitogen activated protein kinase 15; Mitogen-activated protein kinase 15; MK15_HUMAN;

Applications:

WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Predicted Reactivity:

Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-ERK8 (Thr175+Tyr177) Antibody detects endogenous levels of ERK8 only when phosphorylated at Threonine 175 and Tyrosine 177.

RRID:

AB_2833512
Please cite this product as: Affinity Biosciences Cat# AF0029, RRID:AB_2833512.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

MAPK15

Molecular Weight:

Observed Mol.Wt.: 60kD.
Predicted Mol.Wt.: 60kDa(Calculated)..

Tissue Specificity:

Q8TD08 MK15_HUMAN:
Widely expressed with a maximal expression in lung and kidney.

Description:

ERK7 is phosphorylates MBP in vitro. Interacts with CSK/c-Src, ABL1 and RET. 3 isoforms of the human protein are produced by alternative splicing.

Sequence:
MCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEYVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLEGHHV

Research Background

Function:

Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:22948227, PubMed:24618899, PubMed:29021280, PubMed:21847093, PubMed:20733054). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:20638370, PubMed:19166846). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936).

Post-translational Modifications:

Autophosphorylated on Thr-175 and Tyr-177; activates the enzyme.

Ubiquitinated (PubMed:19166846). Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium basal body. Cell junction>Tight junction. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasmic vesicle>Autophagosome. Golgi apparatus. Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle.
Note: Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Widely expressed with a maximal expression in lung and kidney.

Subunit Structure:

Interacts with CSK/c-Src, ABL1, RET and TGFB1I1 (PubMed:11875070, PubMed:16484222, PubMed:16624805). Interacts with GABARAP, MAP1LC3B and GABARAPL1; controls, in a kinase-dependent fashion, both basal and starvation-induced autophagy (PubMed:22948227). Interacts with ESRRA; promotes re-localization of ESRRA to the cytoplasm through a XPO1-dependent mechanism then inhibits ESRRA transcriptional activity (PubMed:21190936). Interacts with PCNA; the interaction is chromatin binding- and kinase activity-dependent and prevents MDM2-mediated PCNA destruction by inhibiting the association of PCNA with MDM2 (PubMed:20733054). Interacts with DVL2 (By similarity). Interacts with CLIC3; MAPK15 does not phosphorylates CLIC3 (By similarity).

Similarity:

The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

Research Fields:

· Organismal Systems > Immune system > IL-17 signaling pathway.(View pathway)

WB Images(1)


IF/ICC Images(1)


ELISA analysis of AF0029 showing specificity to Phospho-ERK8 (Thr175+Tyr177) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.
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Catalog Number :

AF0029-BP
(Blocking peptide available as AF0029-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Zebrafish
100%
Chicken
100%
Rabbit
100%
Xenopus
100%
Dog
100%
Bovine
100%
Sheep
100%
Pig
100%
Horse
0%
High similarity Medium similarity Low similarity No similarity
Q8TD08 as Substrate
Site PTM Type Enzyme
T175 Phosphorylation Q8TD08 (MAPK15)
Y177 Phosphorylation Q8TD08 (MAPK15)
S192 Phosphorylation Q8TD08 (MAPK15)
S331 Phosphorylation Q8TD08 (MAPK15)
Y340 Phosphorylation
T352 Phosphorylation Q8TD08 (MAPK15)
S362 Phosphorylation Q8TD08 (MAPK15)
S379 Phosphorylation Q8TD08 (MAPK15)
T381 Phosphorylation Q8TD08 (MAPK15)
S415 Phosphorylation
R449 Methylation
Q8TD08 as PTM Enzyme
Substrate Site Source
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
Q8TD08 (MAPK15) T175 Uniprot
Q8TD08 (MAPK15) Y177 Uniprot
Q8TD08 (MAPK15) S192 Uniprot
Q8TD08 (MAPK15) S331 Uniprot
Q8TD08 (MAPK15) T352 Uniprot
Q8TD08 (MAPK15) S362 Uniprot
Q8TD08 (MAPK15) S379 Uniprot
Q8TD08 (MAPK15) T381 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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