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  • Product Name
    Phospho-APC (Ser2054) Antibody
  • Catalog No.
    AF0825
  • RRID
    AB_2833510
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse
  • UniProt
  • Mol.Wt
    311kD;
    312kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Adenomatous Polyposis Coli; Adenomatous polyposis coli protein; Apc; APC_HUMAN; CC1; Deleted in polyposis 2.5; DP2; DP2.5; DP3; FAP; FPC; GS; Protein APC;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-APC (Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Sersine 2054.

RRID:

AB_2833510
Please cite this product as: Affinity Biosciences Cat# AF0825, RRID:AB_2833510.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human APC around the phosphorylation site of Ser2054.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

APC

Molecular Weight:

Observed Mol.Wt.: 311kD.
Predicted Mol.Wt.: 312kDa(Calculated)..

Subcellular Location:

Cell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

Tissue Specificity:

P25054 APC_HUMAN:
Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver (PubMed:21643010, PubMed:27217144). Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues (PubMed:19527921, PubMed:21643010, PubMed:27217144). Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood (PubMed:19527921, PubMed:21643010, PubMed:27217144).

Description:

This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Sequence:
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQATEAERSSQNKHETGSHDAERQNEGQGVGEINMATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQSLYGDYVFDTNRHDDNRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLGNYHPATENPGTSSKRGLQISTTAAQIAKVMEEVSAIHTSQEDRSSGSTTELHCVTDERNALRRSSAAHTHSNTYNFTKSENSNRTCSMPYAKLEYKRSSNDSLNSVSSSDGYGKRGQMKPSIESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDKHLKFQPHFGQQECVSPYRSRGANGSETNRVGSNHGINQNVSQSLCQEDDYEDDKPTNYSERYSEEEQHEEEERPTNYSIKYNEEKRHVDQPIDYSLKYATDIPSSQKQSFSFSKSSSGQSSKTEHMSSSSENTSTPSSNAKRQNQLHPSSAQSRSGQPQKAATCKVSSINQETIQTYCVEDTPICFSRCSSLSSLSSAEDEIGCNQTTQEADSANTLQIAEIKEKIGTRSAEDPVSEVPAVSQHPRTKSSRLQGSSLSSESARHKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTAQTKREVPKNKAPTAEKRESGPKQAAVNAAVQRVQVLPDADTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPKESNENQEKEAEKTIDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKPAQTASKLPPPVARKPSQLPVYKLLPSQNRLQPQKHVSFTPGDDMPRVYCVEGTPINFSTATSLSDLTIESPPNELAAGEGVRGGAQSGEFEKRDTIPTEGRSTDEAQGGKTSSVTIPELDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASASSSAPNKNQLDGKKKKPTSPVKPIPQNTEYRTRVRKNADSKNNLNAERVFSDNKDSKKQNLKNNSKVFNDKLPNNEDRVRGSFAFDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKAKENKESEAKVTSHTELTSNQQSANKTQAIAKQPINRGQPKPILQKQSTFPQSSKDIPDRGAATDEKLQNFAIENTPVCFSHNSSLSSLSDIDQENNNKENEPIKETEPPDSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKPSRLKGDNEKHSPRNMGGILGEDLTLDLKDIQRPDSEHGLSPDSENFDWKAIQEGANSIVSSLHQAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLETKKIESESKGIKGGKKVYKSLITGKVRSNSEISGQMKQPLQANMPSISRGRTMIHIPGVRNSSSSTSPVSKKGPPLKTPASKSPSEGQTATTSPRGAKPSVKSELSPVARQTSQIGGSSKAPSRSGSRDSTPSRPAQQPLSRPIQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQMSQQNLTKQTGLSKNASSIPRSESASKGLNQMNNGNGANKKVELSRMSSTKSSGSESDRSERPVLVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPASPTRSQAQTPVLSPSLPDMSLSTHSSVQAGGWRKLPPNLSPTIEYNDGRPAKRHDIARSHSESPSRLPINRSGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDEKHVNSISGTKQSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAESKTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLENRLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV

Research Background

Function:

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Post-translational Modifications:

Phosphorylated by GSK3B.

Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.

Subcellular Location:

Cell junction>Adherens junction. Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cell projection>Ruffle membrane. Cytoplasm. Cell membrane.
Note: Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment (PubMed:19151759). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver (PubMed:21643010, PubMed:27217144). Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues (PubMed:19527921, PubMed:21643010, PubMed:27217144). Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood (PubMed:19527921, PubMed:21643010, PubMed:27217144).

Subunit Structure:

Forms homooligomers and heterooligomers with APC2. Interacts with DIAPH1 and DIAPH2 (By similarity). Interacts with PDZ domains of DLG1 and DLG3. Associates with catenins. Binds axin. Interacts with ARHGEF4 (via N-terminus). Interacts with MAPRE1 (via C-terminus); probably required for APC targeting to the growing microtubule plus ends. Interacts with MAPRE2 and MAPRE3 (via C-terminus). Found in a complex consisting of ARHGEF4, APC and CTNNB1. Interacts with SCRIB; may mediate APC targeting to adherens junctions of epithelial cells. Interacts with SPATA13 (via N-terminus and SH3 domain). Interacts with ASAP1 (via SH3 domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B (By similarity). Interacts at the cell membrane with AMER1 and AMER2 (via ARM repeats). Interacts with KHDRBS1. The complex composed, at least, of APC, CTNNB1 and GSK3B interacts with JPT1; the interaction requires the inactive form of GSK3B (phosphorylated at 'Ser-9') (PubMed:25169422).

Similarity:

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.

Belongs to the adenomatous polyposis coli (APC) family.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway.(View pathway)
· Human Diseases > Cancers: Specific types > Basal cell carcinoma.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.(View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Endometrial cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer.(View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

WB Images(1)


IHC Images(2)


IF/ICC Images(1)


ELISA analysis of AF0825 showing specificity to Phospho-APC (Ser2054) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.
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Catalog Number :

AF0825-BP
(Blocking peptide available as AF0825-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

P25054 as Substrate
Site PTM Type Enzyme
Phosphorylation
Ubiquitination
A2 Acetylation
S5 Phosphorylation
K73 Ubiquitination
S92 Phosphorylation
S95 Phosphorylation
Y96 Phosphorylation
S98 Phosphorylation
S107 Phosphorylation
S111 Phosphorylation
K150 Ubiquitination
T297 Phosphorylation
Y316 Phosphorylation
S317 Phosphorylation
S331 Phosphorylation
T333 Phosphorylation
S552 Phosphorylation
K561 Methylation
K580 Methylation
T600 Phosphorylation
S705 Phosphorylation
S744 Phosphorylation
S748 Phosphorylation
S780 Phosphorylation
S794 Phosphorylation
Y796 Phosphorylation
Y799 Phosphorylation
S851 Phosphorylation
Y863 Phosphorylation
T867 Phosphorylation
T872 Phosphorylation
S874 Phosphorylation
T883 Phosphorylation
S895 Phosphorylation
T899 Phosphorylation
S908 Phosphorylation
S932 Phosphorylation
Y935 Phosphorylation
K939 Ubiquitination
Y951 Phosphorylation
R958 Methylation
S960 Phosphorylation
S963 Phosphorylation
R976 Methylation
S985 Phosphorylation
S987 Phosphorylation
Y997 Phosphorylation
T1023 Phosphorylation
S1032 Phosphorylation
S1038 Phosphorylation
S1042 Phosphorylation
S1044 Phosphorylation
S1063 Phosphorylation
Y1078 Phosphorylation
S1081 Phosphorylation
S1100 Phosphorylation
Y1102 Phosphorylation
S1117 Phosphorylation
Y1183 Phosphorylation
S1194 Phosphorylation
S1196 Phosphorylation
S1214 Phosphorylation
S1215 Phosphorylation
T1218 Phosphorylation
S1219 Phosphorylation
T1220 Phosphorylation
S1222 Phosphorylation
S1223 Phosphorylation
S1238 Phosphorylation
S1272 Phosphorylation
S1278 Phosphorylation
S1279 Phosphorylation P49674 (CSNK1E)
T1292 Phosphorylation
T1301 Phosphorylation
S1315 Phosphorylation
S1341 Phosphorylation
S1343 Phosphorylation
S1344 Phosphorylation
S1346 Phosphorylation
S1355 Phosphorylation
S1360 Phosphorylation P24941 (CDK2)
S1362 Phosphorylation
S1364 Phosphorylation
T1368 Phosphorylation
S1371 Phosphorylation
T1380 Phosphorylation
S1385 Phosphorylation
S1389 Phosphorylation
S1392 Phosphorylation P49674 (CSNK1E)
S1395 Phosphorylation
S1426 Phosphorylation
S1436 Phosphorylation
T1438 Phosphorylation
T1448 Phosphorylation
T1459 Phosphorylation
R1463 Methylation
S1501 Phosphorylation P49841 (GSK3B)
S1503 Phosphorylation P49841 (GSK3B)
S1504 Phosphorylation Q9HCP0 (CSNK1G1) , P48730 (CSNK1D) , P48729 (CSNK1A1)
S1505 Phosphorylation Q9HCP0 (CSNK1G1) , P48729 (CSNK1A1) , P48730 (CSNK1D)
S1507 Phosphorylation P48729 (CSNK1A1) , Q9HCP0 (CSNK1G1) , P48730 (CSNK1D)
S1510 Phosphorylation P48729 (CSNK1A1) , Q9HCP0 (CSNK1G1) , P48730 (CSNK1D)
T1556 Phosphorylation
S1567 Phosphorylation
S1610 Phosphorylation
Y1615 Phosphorylation
T1633 Phosphorylation
S1737 Phosphorylation
T1773 Phosphorylation
S1774 Phosphorylation
S1842 Phosphorylation
Y1846 Phosphorylation
T1847 Phosphorylation
T1852 Phosphorylation
Y1854 Phosphorylation
S1861 Phosphorylation
S1863 Phosphorylation
S1864 Phosphorylation
S1906 Phosphorylation
T1932 Phosphorylation
S2011 Phosphorylation
T2017 Phosphorylation
S2022 Phosphorylation
S2034 Phosphorylation
S2054 Phosphorylation P17612 (PRKACA)
S2064 Phosphorylation
S2088 Phosphorylation
S2093 Phosphorylation
S2096 Phosphorylation
S2110 Phosphorylation
S2113 Phosphorylation
S2125 Phosphorylation
S2129 Phosphorylation
S2130 Phosphorylation
S2132 Phosphorylation
S2140 Phosphorylation
S2143 Phosphorylation
S2146 Phosphorylation
T2151 Phosphorylation
S2183 Phosphorylation
S2185 Phosphorylation
K2189 Acetylation
K2192 Acetylation
S2207 Phosphorylation
R2226 Methylation
T2255 Phosphorylation
S2258 Phosphorylation
S2260 Phosphorylation
S2262 Phosphorylation
T2266 Phosphorylation
T2269 Phosphorylation
S2270 Phosphorylation
S2277 Phosphorylation
S2280 Phosphorylation
S2283 Phosphorylation
S2290 Phosphorylation
S2300 Phosphorylation
T2308 Phosphorylation
S2323 Phosphorylation
S2330 Phosphorylation
S2337 Phosphorylation
Y2366 Phosphorylation
S2368 Phosphorylation
S2374 Phosphorylation
S2390 Phosphorylation
S2396 Phosphorylation
S2398 Phosphorylation
S2421 Phosphorylation
S2441 Phosphorylation
T2442 Phosphorylation
S2449 Phosphorylation
T2451 Phosphorylation
S2461 Phosphorylation
S2464 Phosphorylation
S2469 Phosphorylation
S2473 Phosphorylation
S2512 Phosphorylation
S2533 Phosphorylation
S2535 Phosphorylation
S2537 Phosphorylation
T2546 Phosphorylation
S2569 Phosphorylation
S2570 Phosphorylation
S2571 Phosphorylation
S2580 Phosphorylation
S2581 Phosphorylation
S2586 Phosphorylation
S2594 Phosphorylation
S2607 Phosphorylation
T2611 Phosphorylation
S2621 Phosphorylation
Y2645 Phosphorylation
S2652 Phosphorylation
S2671 Phosphorylation
S2674 Phosphorylation
T2676 Phosphorylation
T2679 Phosphorylation
S2685 Phosphorylation
S2724 Phosphorylation
S2764 Phosphorylation
S2766 Phosphorylation
S2768 Phosphorylation
S2771 Phosphorylation
S2772 Phosphorylation
T2776 Phosphorylation
T2782 Phosphorylation
S2789 Phosphorylation
S2793 Phosphorylation
S2794 Phosphorylation
T2807 Phosphorylation
S2822 Phosphorylation
T2823 Phosphorylation
S2825 Phosphorylation
T2828 Phosphorylation
S2830 Phosphorylation
S2835 Phosphorylation
S2837 Phosphorylation
Y2838 Phosphorylation
T2841 Phosphorylation
S2842 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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