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  • Product Name
    Phospho-CDC25A (Ser76) Antibody
  • Catalog No.
  • RRID
  • Source
  • Application
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)
  • UniProt
  • Mol.Wt
  • Concentration
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Cdc 25a; CDC25A; CDC25A2; CDC25A2 CAG isoform; Cell division cycle 25 homolog A (S. pombe); Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform b; D9Ertd393e; Dual specificity phosphatase Cdc25A; M phase inducer phosphatase 1; M-phase inducer phosphatase 1; MGC115549; MPIP1_HUMAN;


WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.


Human, Mouse, Rat

Predicted Reactivity:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)






The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.


Phospho-CDC25A (Ser76) Antibody detects endogenous levels of CDC25A only when phosphorylated at Sersine 76.


Please cite this product as: Affinity Biosciences Cat# AF0906, RRID:AB_2833509.





Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information


A synthesized peptide derived from human CDC25A around the phosphorylation site of Ser76.


>>Visit The Human Protein Atlas

Gene ID:

Gene Name:


Molecular Weight:

Observed Mol.Wt.: 59kD.
Predicted Mol.Wt.: 59kDa(Calculated)..


CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.


Research Background


Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Post-translational Modifications:

Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.

Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

Subunit Structure:

Interacts with CCNB1/cyclin B1. Interacts with YWHAE/14-3-3 epsilon when phosphorylated. Interacts with CUL1 specifically when CUL1 is neddylated and active. Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage. Interacts with PIM1. Interacts with CHEK2; mediates CDC25A phosphorylation and degradation in response to infrared-induced DNA damages. Interacts with HSP90AB1; prevents heat shock-mediated CDC25A degradation and contributes to cell cycle progression (PubMed:22843495).


The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.

Belongs to the MPI phosphatase family.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > Cell cycle.(View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence.(View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

WB Images(1)

IHC Images(1)

IF/ICC Images(1)

ELISA analysis of AF0906 showing specificity to Phospho-CDC25A (Ser76) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.
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Catalog Number :

(Blocking peptide available as AF0906-BP)

Price/Size :

Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

High similarity Medium similarity Low similarity No similarity
P30304 as Substrate
Site PTM Type Enzyme
S18 Phosphorylation P06493 (CDK1)
S40 Phosphorylation
Y59 Phosphorylation
S76 Phosphorylation O14757 (CHEK1) , P49841 (GSK3B)
S77 Phosphorylation
S79 Phosphorylation Q8NG66 (NEK11) , P48729 (CSNK1A1)
T80 Phosphorylation Q9H4B4 (PLK3)
S82 Phosphorylation P48729 (CSNK1A1) , Q8NG66 (NEK11)
S88 Phosphorylation Q8NG66 (NEK11)
S107 Phosphorylation
K111 Ubiquitination
S116 Phosphorylation P06493 (CDK1)
K120 Ubiquitination
S124 Phosphorylation Q683Z8 (CHK2) , O96017 (CHEK2) , O14757 (CHEK1)
S126 Phosphorylation
K150 Acetylation
K150 Ubiquitination
K169 Ubiquitination
S178 Phosphorylation Q683Z8 (CHK2) , O14757 (CHEK1) , O96017 (CHEK2)
K257 Ubiquitination
S261 Phosphorylation
S263 Phosphorylation P24941 (CDK2)
R278 Methylation
S279 Phosphorylation Q683Z8 (CHK2) , O14757 (CHEK1) , O96017 (CHEK2)
S283 Phosphorylation
R290 Methylation
S293 Phosphorylation O14757 (CHEK1) , O96017 (CHEK2) , Q15418 (RPS6KA1) , Q683Z8 (CHK2)
S295 Phosphorylation Q15418 (RPS6KA1)
K300 Ubiquitination
S321 Phosphorylation
K343 Ubiquitination
K353 Ubiquitination
K358 Ubiquitination
Y359 Phosphorylation
S361 Phosphorylation
S367 Phosphorylation
K372 Ubiquitination
K378 Ubiquitination
K415 Ubiquitination
K471 Ubiquitination
Y486 Phosphorylation
T507 Phosphorylation O14757 (CHEK1)
S513 Phosphorylation Q9H4B4 (PLK3)
Y518 Phosphorylation
S519 Phosphorylation Q9H4B4 (PLK3)
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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