MMP16 Antibody - #AF0214

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Product: MMP16 Antibody
Catalog: AF0214
Description: Rabbit polyclonal antibody to MMP16
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 69kDa; 70kD(Calculated).
Uniprot: P51512
RRID: AB_2833284

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MMP16 Antibody detects endogenous levels of total MMP16.
RRID:
AB_2833284
Cite Format: Affinity Biosciences Cat# AF0214, RRID:AB_2833284.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C8orf57; DKFZp761D112; Matrix metalloproteinase 16; Matrix metalloproteinase-16; Membrane type 3 matrix metalloproteinase; Membrane type matrix metalloproteinase 3; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP 16; MMP-16; MMP-X2; MMP16; MMP16_HUMAN; MT-MMP 3; MT-MMP2; MT-MMP3; MT3-MMP; MT3MMP; MTMMP2; MTMMP3; Putative transmembrane protein C8orf57;

Immunogens

Immunogen:
Uniprot:

P51512(MMP16_HUMAN) >>Visit HPA database.

Gene(ID):
MMP16(4325)
Expression:
P51512 MMP16_HUMAN:

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Description:
MMP16 Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen.
Sequence:
MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51512 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
T8 Phosphorylation
Y52 Phosphorylation
S61 Phosphorylation
S65 Phosphorylation
T92 Phosphorylation
Y134 Phosphorylation
Y377 Phosphorylation
T382 Phosphorylation
T478 Phosphorylation
Y521 Phosphorylation

Research Backgrounds

Function:

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

PTMs:

The precursor is cleaved by a furin endopeptidase.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein>Extracellular side.
Note: Localized at the cell surface of melanoma cells.

Secreted>Extracellular space>Extracellular matrix. Cell surface.
Note: Localized at the cell surface of melanoma cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Subunit Structure:

Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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