AKAP10 Antibody - #AF0330

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Product: AKAP10 Antibody
Catalog: AF0330
Description: Rabbit polyclonal antibody to AKAP10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 73kDa; 74kD(Calculated).
Uniprot: O43572
RRID: AB_2833492

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AKAP10 Antibody detects endogenous levels of total AKAP10.
RRID:
AB_2833492
Cite Format: Affinity Biosciences Cat# AF0330, RRID:AB_2833492.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1500031L16Rik; A kinase (PRKA) anchor protein 10; A kinase anchor protein 10; A kinase anchor protein 10 mitochondrial; A-kinase anchor protein 10; AKA10_HUMAN; akap 10; AKAP-10; AKAP10; B130049N18Rik; D AKAP 2; D-AKAP-2; dakap 2; dakap2; Dual specific A kinase anchoring protein 2; Dual specificity A kinase-anchoring protein 2; mgc9410; MGC9414|; Mitochondrial A kinase PPKA anchor protein 10; mitochondrial; prka 10; PRKA10; Protein A kinase anchoring protein 10; Protein A kinase anchoring protein 10 mitochondrial precursor; Protein kinase A anchoring protein 10; Protein kinase A-anchoring protein 10;

Immunogens

Immunogen:
Uniprot:

O43572(AKA10_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP10(11216)
Description:
AKAP10 Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA.
Sequence:
MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Pig
73
Xenopus
70
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43572 As Substrate

Site PTM Type Enzyme
R2 Methylation
S7 Phosphorylation
R9 Methylation
S11 Phosphorylation
S24 Phosphorylation
S47 Phosphorylation
S52 Phosphorylation
K55 Methylation
S56 Phosphorylation
T90 Phosphorylation
K93 Ubiquitination
S96 Phosphorylation
Y113 Phosphorylation
K119 Ubiquitination
S173 Phosphorylation
S187 Phosphorylation
S189 Phosphorylation
S197 Phosphorylation
S202 Phosphorylation
T229 Phosphorylation
S239 Phosphorylation
S261 Phosphorylation
S267 Phosphorylation
S269 Phosphorylation
T272 Phosphorylation
S278 Phosphorylation
S281 Phosphorylation
K289 Ubiquitination
K292 Ubiquitination
S293 Phosphorylation
K312 Ubiquitination
K423 Ubiquitination
Y442 Phosphorylation
T448 Phosphorylation
K559 Ubiquitination
K585 Ubiquitination
K611 Ubiquitination
K621 Ubiquitination
K637 Ubiquitination
K658 Ubiquitination

Research Backgrounds

Function:

Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).

Subcellular Location:

Mitochondrion. Membrane. Cytoplasm.
Note: Predominantly mitochondrial but also membrane associated and cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Restrictive clause

 

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For Research Use Only.
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