PHF1 Antibody - #AF0324
| Product: | PHF1 Antibody |
| Catalog: | AF0324 |
| Description: | Rabbit polyclonal antibody to PHF1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Rabbit |
| Mol.Wt.: | 62kDa; 62kD(Calculated). |
| Uniprot: | O43189 |
| RRID: | AB_2833487 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0324, RRID:AB_2833487.
Fold/Unfold
hPCl1; MTF2 L2; MTF2L 2; MTF2L2; PCL 1; PCL1; PHD finger protein 1; PHD finger protein1; PHF 1; PHF 2; Phf1; PHF1_HUMAN; PHF2; Plc 1; Plc1; Polycomb like protein 1; Polycomb like protein1; Polycomb-like protein 1; Protein PHF1; T complex testis expressed 3; T complex testis expressed3; Tctex 3; Tctex3;
Immunogens
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
- O43189 PHF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQPPRLSRSGASSLWDPASPAPTSGPRPRLWEGQDVLARWTDGLLYLGTIKKVDSAREVCLVQFEDDSQFLVLWKDISPAALPGEELLCCVCRSETVVPGNRLVSCEKCRHAYHQDCHVPRAPAPGEGEGTSWVCRQCVFAIATKRGGALKKGPYARAMLGMKLSLPYGLKGLDWDAGHLSNRQQSYCYCGGPGEWNLKMLQCRSCLQWFHEACTQCLSKPLLYGDRFYEFECCVCRGGPEKVRRLQLRWVDVAHLVLYHLSVCCKKKYFDFDREILPFTSENWDSLLLGELSDTPKGERSSRLLSALNSHKDRFISGREIKKRKCLFGLHARMPPPVEPPTGDGALTSFPSGQGPGGGVSRPLGKRRRPEPEPLRRRQKGKVEELGPPSAVRNQPEPQEQRERAHLQRALQASVSPPSPSPNQSYQGSSGYNFRPTDARCLPSSPIRMFASFHPSASTAGTSGDSGPPDRSPLELHIGFPTDIPKSAPHSMTASSSSVSSPSPGLPRRSAPPSPLCRSLSPGTGGGVRGGVGYLSRGDPVRVLARRVRPDGSVQYLVEWGGGGIF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43189 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| R530 | Methylation | Uniprot |
Research Backgrounds
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 lociand. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Co-localizes with NEK6 in the centrosome.
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
Interacts with CHMP1 (By similarity). Associated component of the PRC2 complex. Interacts with p53/TP53.
The Tudor domain recognizes and binds H3K36me3 (PubMed:23228662, PubMed:23273982 and PubMed:23142980).
Belongs to the Polycomblike family.
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