TBX 2/3 Antibody - #AF0319

(2)  
Product: TBX 2/3 Antibody
Catalog: AF0319
Description: Rabbit polyclonal antibody to TBX 2/3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 79kDa; 79kD,75kD(Calculated).
Uniprot: O15119 | Q13207
RRID: AB_2833482

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TBX 2/3 Antibody detects endogenous levels of total TBX 2/3.
RRID:
AB_2833482
Cite Format: Affinity Biosciences Cat# AF0319, RRID:AB_2833482.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bladder cancer related protein XHL; T box 2; T box 3; T box protein 2; T box protein 3; T box transcription factor TBX2; T box transcription factor TBX3; TBX3-ISO; UMS; XHL; FLJ10169; HGNC:11597; T box 2; T box transcription factor TBX2; T-box protein 2; T-box transcription factor TBX2; TBX2; TBX2_HUMAN;

Immunogens

Immunogen:
Uniprot:

O15119(TBX3_HUMAN) >>Visit HPA database.

Q13207(TBX2_HUMAN) >>Visit HPA database.

Gene(ID):
TBX2(6909) | TBX3(6926)
Expression:
O15119 TBX3_HUMAN:

Widely expressed.

Q13207 TBX2_HUMAN:

Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.

Description:
TBX3 Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. 4 isoforms of the human protein are produced by alternative splicing.
Sequence:
MSLSMRDPVIPGTSMAYHPFLPHRAPDFAMSAVLGHQPPFFPALTLPPNGAAALSLPGALAKPIMDQLVGAAETGIPFSSLGPQAHLRPLKTMEPEEEVEDDPKVHLEAKELWDQFHKRGTEMVITKSGRRMFPPFKVRCSGLDKKAKYILLMDIIAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVTFHKLKLTNNISDKHGFTLAFPSDHATWQGNYSFGTQTILNSMHKYQPRFHIVRANDILKLPYSTFRTYLFPETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLQSMRVFDERHKKENGTSDESSSEQAAFNCFAQASSPAASTVGTSNLKDLCPSEGESDAEAESKEEHGPEACDAAKISTTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP

MREPALAASAMAYHPFHAPRPADFPMSAFLAAAQPSFFPALALPPGALAKPLPDPGLAGAAAAAAAAAAAAEAGLHVSALGPHPPAAHLRSLKSLEPEDEVEDDPKVTLEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLPSLRLYEEHCKPERDGAESDASSCDPPPAREPPTSPGAAPSPLRLHRARAEEKSCAADSDPEPERLSEERAGAPLGRSPAPDSASPTRLTEPERARERRSPERGKEPAESGGDGPFGLRSLEKERAEARRKDEGRKEAAEGKEQGLAPLVVQTDSASPLGAGHLPGLAFSSHLHGQQFFGPLGAGQPLFLHPGQFTMGPGAFSAMGMGHLLASVAGGGNGGGGGPGTAAGLDAGGLGPAASAASTAAPFPFHLSQHMLASQGIPMPTFGGLFPYPYTYMAAAAAAASALPATSAAAAAAAAAGSLSRSPFLGSARPRLRFSPYQIPVTIPPSTSLLTTGLASEGSKAAGGNSREPSPLPELALRKVGAPSRGALSPSGSAKEAANELQSIQRLVSGLESQRALSPGRESPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15119/Q13207 As Substrate

Site PTM Type Enzyme
K104 Ubiquitination
K110 Ubiquitination
K118 Ubiquitination
T121 Phosphorylation
K127 Ubiquitination
Y163 Phosphorylation
Y185 Phosphorylation
S190 Phosphorylation
T203 Phosphorylation
K206 Ubiquitination
K208 Ubiquitination
Y265 Phosphorylation
K293 Ubiquitination
K301 Ubiquitination
K313 Ubiquitination
S371 Phosphorylation
S375 Phosphorylation
S381 Phosphorylation
S396 Phosphorylation
S400 Phosphorylation
S409 Phosphorylation
K413 Ubiquitination
S432 Phosphorylation
S435 Phosphorylation
S438 Phosphorylation
T441 Phosphorylation
S443 Phosphorylation
S444 Phosphorylation
S445 Phosphorylation
T446 Phosphorylation
S456 Phosphorylation
T462 Phosphorylation
K466 Ubiquitination
S680 Phosphorylation
S707 Phosphorylation
K714 Ubiquitination
T718 Phosphorylation
S719 Phosphorylation Q9Y243 (AKT3)
S729 Phosphorylation
K734 Ubiquitination
S738 Phosphorylation
S740 Phosphorylation
S742 Phosphorylation
Site PTM Type Enzyme
S94 Phosphorylation
Y165 Phosphorylation
K210 Ubiquitination
Y247 Phosphorylation
T252 Phosphorylation
Y253 Phosphorylation
T258 Phosphorylation
K275 Ubiquitination
K283 Ubiquitination
K295 Ubiquitination
S320 Phosphorylation
S323 Phosphorylation
S324 Phosphorylation
S336 Phosphorylation
S342 Phosphorylation
S360 Phosphorylation
S368 Phosphorylation
S379 Phosphorylation
S384 Phosphorylation
S386 Phosphorylation
T391 Phosphorylation
S401 Phosphorylation
S635 Phosphorylation
T638 Phosphorylation
S643 Phosphorylation
S646 Phosphorylation
S653 Phosphorylation
S657 Phosphorylation
S676 Phosphorylation
S705 Phosphorylation
S710 Phosphorylation

Research Backgrounds

Function:

Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with PML (isoform PML-4).

Function:

Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence. May be required for cardiac atrioventricular canal formation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.

Subunit Structure:

Interacts with PML (isoform-PML-2, isoform PML-3 and isoform PML-4).

Family&Domains:

The repression domain 1 (RD1) is necessary for its interaction with PML.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

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