KLF11 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/7

Western blot analysis of extracts from 293, using KLF11 Antibody.

Western blot analysis of extracts from Rat liver, using KLF11 Antibody.

AF0315 at 1/100 staining Mouse brain tissue by IHC-P.

AF0315 at 1/100 staining Mouse testis tissue by IHC-P.

AF0315 at 1/100 staining Rat brain tissue by IHC-P.

AF0315 staining Hela by IF/ICC.

Fig.

Product:	KLF11 Antibody
Catalog:	AF0315
Description:	Rabbit polyclonal antibody to KLF11
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	55kDa; 55kD(Calculated).
Uniprot:	O14901
RRID:	AB_2833479

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

KLF11 Antibody detects endogenous levels of total KLF11.

RRID:

AB_2833479
Cite Format: Affinity Biosciences Cat# AF0315, RRID:AB_2833479.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

9830142A17; D12Ertd427e; FKLF; FKLF1; Klf11; KLF11_HUMAN; Krueppel like factor 11; Krueppel-like factor 11; Krueppel-like transcription factor 1; MODY7; Tcfcp2l2; TGFB Early Growth Response 2; TGFB-inducible early growth response protein 2; TGFB-inducible early growth response protein 2b; TGFB-inducible early growth response protein 3; TIEG 2; TIEG-2; TIEG-3; Tieg2b; Transforming Growth Factor Beta Inducible Early Growth Response 2; Transforming growth factor-beta-inducible early growth response protein 2; Transforming growth factor-beta-inducible early growth response protein 3;

Immunogens

Immunogen:

Uniprot:

O14901(KLF11_HUMAN) >>Visit HPA database.

Gene(ID):

KLF11(8462)

Expression:

O14901 KLF11_HUMAN:

Ubiquitous. Higher expression in erythroid cells.

Description:

TIEG2 Transcription factor. Activates the epsilon- and gamma- globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling.

Sequence:

O14901 KLF11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHTPDFAGPDDARAVDIMDICESILERKRHDSERSTCSILEQTDMEAVEALVCMSSWGQRSQKGDLLRIRPLTPVSDSGDVTTTVHMDAATPELPKDFHSLSTLCITPPQSPDLVEPSTRTPVSPQVTDSKACTATDVLQSSAVVARALSGGAERGLLGLEPVPSSPCRAKGTSVIRHTGESPAACFPTIQTPDCRLSDSREGEEQLLGHFETLQDTHLTDSLLSTNLVSCQPCLHKSGGLLLTDKGQQAGWPGAVQTCSPKNYENDLPRKTTPLISVSVPAPPVLCQMIPVTGQSSMLPAFLKPPPQLSVGTVRPILAQAAPAPQPVFVGPAVPQGAVMLVLPQGALPPPAPCAANVMAAGNTKLLPLAPAPVFITSSQNCVPQVDFSRRRNYVCSFPGCRKTYFKSSHLKAHLRTHTGEKPFNCSWDGCDKKFARSDELSRHRRTHTGEKKFVCPVCDRRFMRSDHLTKHARRHMTTKKIPGWQAEVGKLNRIASAESPGSPLVSMPASA

PTMs - O14901 As Substrate

O14901

Site	PTM Type	Source
T73	Phosphorylation	Uniprot
T82	Phosphorylation	Uniprot
T83	Phosphorylation	Uniprot
T107	Phosphorylation	Uniprot
S111	Phosphorylation	Uniprot
S124	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
S166	Phosphorylation	Uniprot
K403	Ubiquitination	Uniprot
K412	Sumoylation	Uniprot
K412	Ubiquitination	Uniprot
T417	Phosphorylation	Uniprot
T419	Phosphorylation	Uniprot
T447	Phosphorylation	Uniprot
T449	Phosphorylation	Uniprot
K471	Ubiquitination	Uniprot

Research Backgrounds

O14901_KLF11_HUMAN

Function:

Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling (By similarity). Induces apoptosis (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitous. Higher expression in erythroid cells.

Subunit Structure:

Interacts with SIN3A.

Family&Domains:

Belongs to the Sp1 C2H2-type zinc-finger protein family.

References

1). Ferroptosis inhibitor alleviates sorafenib-induced cardiotoxicity by attenuating KLF11-mediated FSP1-dependent ferroptosis. International journal of biological sciences, 2024 (PubMed: 38725840) [IF=9.2]

2). KLF11 regulates lung adenocarcinoma ferroptosis and chemosensitivity by suppressing GPX4. Communications Biology, 2023 (PubMed: 37248295) [IF=5.9]

Application: WB Species: Human Sample: A549 and PC9 cell

Fig. 1: Identification of KLF11 as involved in ferroptosis in LUAD. a Schematic diagram of RNA-seq screening workflow in A549 cell line. b, c Volcano plot illustrating KLF11 involved in regulating ferroptosis induced by RSL3 and IKE. d, e KLF11 RNA level was measured after treatment with RSL3 or DFO + RSL3 or Ferr1 + RSL3 in A549 and PC9 cell lines. f, g KLF11 protein level was measured after treatment with RSL3 or DFO + RSL3 or Ferr1 + RSL3 in A549 and PC9 cell lines.

3). The induction of ferroptosis by KLF11/NCOA4 axis: the inhibitory role in clear cell renal cell carcinoma. Human cell, 2023 (PubMed: 37642832) [IF=4.3]

4). Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm. Biochemical genetics, 2024 (PubMed: 38368567) [IF=2.4]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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KLF11 Antibody - #AF0315