BCKD Antibody - #AF0314
| Product: | BCKD Antibody |
| Catalog: | AF0314 |
| Description: | Rabbit polyclonal antibody to BCKD |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 46kDa; 46kD(Calculated). |
| Uniprot: | O14874 |
| RRID: | AB_2833478 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0314, RRID:AB_2833478.
Fold/Unfold
[3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; BCKD kinase; BCKD-kinase; BCKD_HUMAN; BCKDHKIN; Bckdk; BCKDKD; BDK; Branched chain alpha keto acid dehydrogenase kinase; Branched chain ketoacid dehydrogenase kinase; Branched-chain alpha-ketoacid dehydrogenase kinase; mitochondrial;
Immunogens
- O14874 BCKD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14874 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S31 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| K89 | Methylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K184 | Acetylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K245 | Acetylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
Autophosphorylated.
Mitochondrion matrix. Mitochondrion.
Ubiquitous.
Monomer.
Belongs to the PDK/BCKDK protein kinase family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.