AFfirm™ FHL5 Mouse Monoclonal Antibody - #BF8821
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Protocols
Product Info
Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Monoclonal [AFfirm8821]
Specificity:
FHL5 Mouse Monoclonal Antibody detects endogenous levels of FHL5.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Immunogens
Immunogen:
A synthesized peptide derived from human FHL5.
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q5TD97 FHL5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDRVFSNYCEECKKPIESDSKDLCYKDRHWHEGCFKCTKCNHSLVEKPFAAKDERLLCTECYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFCDQLWHKECFLCSGCRKDLCEEQFMSRDDYPFCVDCYNHLYANKCVACSKPISGLTGAKFICFQDSQWHSECFNCGKCSVSLVGKGFLTQNKEIFCQKCGSGMDTDI
Research Backgrounds
Function:
May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner.
Subcellular Location:
Nucleus.
Note: Nuclei of round and elongated spermatids.
Tissue Specificity:
Testis-specific (at protein level).
Subunit Structure:
Interacts with CREM (via the third LIM domain). Interacts (via second LIM domain) with SPAG8.
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For Research Use Only.
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