TRIM38 Antibody - #AF0307
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TRIM38 Antibody detects endogenous levels of total TRIM38.
RRID:
AB_2833471
Cite Format: Affinity Biosciences Cat# AF0307, RRID:AB_2833471.
Cite Format: Affinity Biosciences Cat# AF0307, RRID:AB_2833471.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Gm23; MGC8946; RING finger protein 15; RNF15; Ro/SSA ribonucleoprotein homolog; RORET; RP23 480B19.4; tripartite motif containing 38; Zinc finger protein RoRet;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.
Sequence:
- O00635 TRI38_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASTTSTKKMMEEATCSICLSLMTNPVSINCGHSYCHLCITDFFKNPSQKQLRQETFCCPQCRAPFHMDSLRPNKQLGSLIEALKETDQEMSCEEHGEQFHLFCEDEGQLICWRCERAPQHKGHTTALVEDVCQGYKEKLQKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLSRLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLYFDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFEVDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEAGVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Dog
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00635 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K167 | Acetylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
E3 ubiquitin-protein ligase. Mediates 'Lys-48'-linked polyubiquitination and proteasomal degradation of the critical TLR adapter TICAM1, inhibiting TLR3-mediated type I interferon signaling.
Tissue Specificity:
Ubiquitous.
References
1). Tripartite motif 38 attenuates cardiac fibrosis after myocardial infarction by suppressing TAK1 activation via TAB2/3 degradation. iScience, 2022
(PubMed: 35982795)
[IF=5.8]
Application: WB Species: Mouse Sample: cardiac fibrosis
Figure 2 TRIM38 inhibits the transformation of cardiac fibroblasts to myofibroblasts in vitro (A and B) Representative Western blotting (Left) and quantitative results (Right) of TRIM38 protein expression in NRCFs transfected with AdshTRIM38 or AdTRIM38. Three independent experiments; ∗p < 0.05. (C and D) Representative Western blotting (Left) and quantitative results (Right) of collagen I and α-SMA protein expression in NRCFs transfected with AdshTRIM38 or AdTRIM38 stimulated with angiotensin Ⅱ. Three independent experiments; ∗p < 0.05; ∗∗p < 0.01. (E and F) Representative immunofluorescence staining image of collagen I and α-SMA in NRCFs stimulated with angiotensin Ⅱ or not. NRCFs transfected with AdshTRIM38 or AdTRIM38. Scale bar = 50 μm. (G and H) Migration ability of transfected with AdshTRIM38 or AdTRIM38 derived from indicated NRCFs detected by wound healing assay. Three independent experiments; ∗p < 0.05. The result was means ± S.D. Statistics in A and B was performed using unpaired Student t-test. Statistics in C, D and G, H were performed using a one-way ANOVA.
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