Product: PRC1 Mouse Monoclonal Antibody
Catalog: BF8385
Description: Mouse monoclonal antibody to PRC1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Xenopus
Mol.Wt.: 72kDa; 72kD(Calculated).
Uniprot: O43663

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8385]
Specificity:
PRC1 Mouse Monoclonal Antibody detects endogenous levels of total PRC1
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Anaphase spindle elongation 1 homolog; ASE1; PRC1; PRC1_HUMAN; Protein regulating cytokinesis 1; Protein regulator of cytokinesis 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O43663 PRC1_HUMAN:

Overexpressed in bladder cancer cells (PubMed:17409436).

Sequence:
MRRSEVLAEESIVCLQKALNHLREIWELIGIPEDQRLQRTEVVKKHIKELLDMMIAEEESLKERLIKSISVCQKELNTLCSELHVEPFQEEGETTILQLEKDLRTQVELMRKQKKERKQELKLLQEQDQELCEILCMPHYDIDSASVPSLEELNQFRQHVTTLRETKASRREEFVSIKRQIILCMEALDHTPDTSFERDVVCEDEDAFCLSLENIATLQKLLRQLEMQKSQNEAVCEGLRTQIRELWDRLQIPEEEREAVATIMSGSKAKVRKALQLEVDRLEELKMQNMKKVIEAIRVELVQYWDQCFYSQEQRQAFAPFCAEDYTESLLQLHDAEIVRLKNYYEVHKELFEGVQKWEETWRLFLEFERKASDPNRFTNRGGNLLKEEKQRAKLQKMLPKLEEELKARIELWEQEHSKAFMVNGQKFMEYVAEQWEMHRLEKERAKQERQLKNKKQTETEMLYGSAPRTPSKRRGLAPNTPGKARKLNTTTMSNATANSSIRPIFGGTVYHSPVSRLPPSGSKPVAASTCSGKKTPRTGRHGANKENLELNGSILSGGYPGSAPLQRNFSINSVASTYSEFAKDPSLSDSSTVGLQRELSKASKSDATSGILNSTNIQS

PTMs - O43663 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K17 Ubiquitination
K67 Ubiquitination
S70 Phosphorylation
K74 Ubiquitination
K101 Ubiquitination
T105 Phosphorylation
R111 Methylation
K178 Ubiquitination
S195 Phosphorylation
K229 Ubiquitination
R249 Methylation
T262 Phosphorylation
S265 Phosphorylation
S267 Phosphorylation
K268 Ubiquitination
K273 Ubiquitination
R281 Methylation
K286 Ubiquitination
K349 Ubiquitination
T379 Phosphorylation
K387 Sumoylation
K387 Ubiquitination
K390 Ubiquitination
K401 Sumoylation
K401 Ubiquitination
K419 Ubiquitination
K453 Acetylation
K456 Ubiquitination
Y464 Phosphorylation
S466 Phosphorylation
T470 Phosphorylation
S472 Phosphorylation
R475 Methylation
T481 Phosphorylation P06493 (CDK1)
K484 Ubiquitination
K487 Ubiquitination
T509 Phosphorylation
Y511 Phosphorylation
S513 Phosphorylation
S516 Phosphorylation
S521 Phosphorylation
S523 Phosphorylation
K524 Ubiquitination
S529 Phosphorylation
T530 Phosphorylation
S532 Phosphorylation
K534 Acetylation
T536 Phosphorylation
S554 Phosphorylation
S557 Phosphorylation
Y560 Phosphorylation
S563 Phosphorylation
S571 Phosphorylation
S574 Phosphorylation
S577 Phosphorylation
T578 Phosphorylation
S587 Phosphorylation
S589 Phosphorylation
S591 Phosphorylation
S592 Phosphorylation
T593 Phosphorylation
K602 Ubiquitination
K605 Ubiquitination
S615 Phosphorylation P53350 (PLK1)
T616 Phosphorylation P53350 (PLK1)
S620 Phosphorylation

Research Backgrounds

Function:

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.

PTMs:

Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. Phosphorylation by CDK1 prevents PLK1-binding: upon degradation of CDK1 at anaphase and dephosphorylation, it is then phosphorylated by PLK1, leading to cytokinesis.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Midbody.
Note: Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Overexpressed in bladder cancer cells.

Subunit Structure:

Homodimer. Interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. Interacts with KIF14 and KIF20A. Interacts with PLK1. Interacts with KIF20B.

Family&Domains:

Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.

Belongs to the MAP65/ASE1 family.

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