Caldesmon Antibody - #AF6411

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Product: Caldesmon Antibody
Catalog: AF6411
Description: Rabbit polyclonal antibody to Caldesmon
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 70~85kD,120~150kD; 93kD(Calculated).
Uniprot: Q05682
RRID: AB_2835241

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Caldesmon Antibody detects endogenous levels of total Caldesmon.
RRID:
AB_2835241
Cite Format: Affinity Biosciences Cat# AF6411, RRID:AB_2835241.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAD; CALD 1; CALD1; CALD1_HUMAN; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22;

Immunogens

Immunogen:
Uniprot:

Q05682(CALD1_HUMAN) >>Visit HPA database.

Gene(ID):
CALD1(800)
Expression:
Q05682 CALD1_HUMAN:

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Description:
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction.
Sequence:
MDDFERRRELRRQKREEMRLEAERIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNAQNSVPDEEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQNDTAENETTEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQVEVMVEEKTTESQEETVVMSLKNGQISSEEPKQEEEREQGSDEISHHEKMEEEDKERAEAERARLEAEERERIKAEQDKKIADERARIEAEEKAAAQERERREAEERERMREEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRARAEEEEKAKVEEQKRNKQLEEKKHAMQETKIKGEKVEQKIEGKWVNEKKAQEDKLQTAVLKKQGEEKGTKVQAKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTEVKSQNGEFMTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDDKKPFKCFTPKGSSLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTSPTKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05682 As Substrate

Site PTM Type Enzyme
Y27 Phosphorylation
S58 Phosphorylation
S73 Phosphorylation Q15746 (MYLK)
T83 Phosphorylation
T84 Phosphorylation
T86 Phosphorylation
K119 Ubiquitination
T124 Phosphorylation
S129 Phosphorylation
S131 Phosphorylation
T141 Phosphorylation
S153 Phosphorylation
Y160 Phosphorylation
K170 Ubiquitination
Y172 Phosphorylation
K174 Ubiquitination
K197 Sumoylation
S202 Phosphorylation
S219 Phosphorylation
S248 Phosphorylation
T402 Phosphorylation
K427 Acetylation
K479 Ubiquitination
S493 Phosphorylation
T506 Phosphorylation
T509 Phosphorylation
S511 Phosphorylation
S518 Phosphorylation
T521 Phosphorylation
K534 Acetylation
T546 Phosphorylation
S548 Phosphorylation
K569 Ubiquitination
S628 Phosphorylation
T638 Phosphorylation
K640 Acetylation
S642 Phosphorylation
S643 Phosphorylation P17252 (PRKCA) , Q9UQM7 (CAMK2A)
K645 Acetylation
K645 Sumoylation
K655 Acetylation
K655 Ubiquitination
S656 Phosphorylation P17252 (PRKCA) , Q9UQM7 (CAMK2A)
S660 Phosphorylation
S661 Phosphorylation
T666 Phosphorylation Q15746 (MYLK)
S673 Phosphorylation
K674 Ubiquitination
S677 Phosphorylation P17252 (PRKCA)
Y682 Phosphorylation
S684 Phosphorylation
K690 Ubiquitination
S691 Phosphorylation
T695 Phosphorylation
S700 Phosphorylation Q15746 (MYLK)
K713 Ubiquitination
S714 Phosphorylation O75914 (PAK3) , Q13177 (PAK2)
K718 Ubiquitination
S723 Phosphorylation
S724 Phosphorylation
T726 Phosphorylation
T730 Phosphorylation P24941 (CDK2) , Q00534 (CDK6)
K733 Acetylation
K733 Ubiquitination
T735 Phosphorylation Q15746 (MYLK)
S744 Phosphorylation Q15746 (MYLK) , Q13177 (PAK2) , O75914 (PAK3)
K752 Acetylation
T753 Phosphorylation
K758 Acetylation
S759 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S765 Phosphorylation
S773 Phosphorylation
K775 Methylation
K775 Ubiquitination
S783 Phosphorylation
T788 Phosphorylation
S789 Phosphorylation P27361 (MAPK3) , Q00534 (CDK6) , P28482 (MAPK1)
T791 Phosphorylation

Research Backgrounds

Function:

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

PTMs:

In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril. Cytoplasm>Cytoskeleton>Stress fiber.
Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Family&Domains:

The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.

Belongs to the caldesmon family.

Research Fields

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

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