Uba2 Antibody - #AF0287

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Product: Uba2 Antibody
Catalog: AF0287
Description: Rabbit polyclonal antibody to Uba2
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 71kDa; 71kD(Calculated).
Uniprot: Q9UBT2
RRID: AB_2833455

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(85%), Bovine(86%), Horse(86%), Sheep(86%)
Clonality:
Polyclonal
Specificity:
Uba2 Antibody detects endogenous levels of total Uba2.
RRID:
AB_2833455
Cite Format: Affinity Biosciences Cat# AF0287, RRID:AB_2833455.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Anthracycline associated resistance ARX; Anthracycline-associated resistance ARX; ARX; FLJ13058; HRIHFB2115; SAE 2; SAE2; SAE2_HUMAN; SUMO 1 activating enzyme subunit 2; SUMO activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2; UBA2 ubiquitin activating enzyme E1 homolog; Ubiquitin like 1 activating enzyme E1B; Ubiquitin like modifier activating enzyme 2; Ubiquitin-like 1-activating enzyme E1B; UBLE1B;

Immunogens

Immunogen:
Uniprot:

Q9UBT2(SAE2_HUMAN) >>Visit HPA database.

Gene(ID):
UBA2(10054)
Description:
SAE2 a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.
Sequence:
MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
86
Bovine
86
Sheep
86
Pig
85
Dog
79
Rabbit
64
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBT2 As Substrate

Site PTM Type Enzyme
K65 Ubiquitination
K77 Acetylation
K77 Ubiquitination
K86 Ubiquitination
K152 Ubiquitination
K153 Ubiquitination
T156 Phosphorylation
Y159 Phosphorylation
K164 Sumoylation
K164 Ubiquitination
K190 Sumoylation
S207 Phosphorylation
R210 Methylation
S229 Phosphorylation
K236 Acetylation
K236 Sumoylation
K236 Ubiquitination
K241 Acetylation
K241 Ubiquitination
K245 Ubiquitination
Y249 Phosphorylation
K253 Acetylation
K253 Sumoylation
K253 Ubiquitination
K257 Sumoylation
K257 Ubiquitination
K260 Ubiquitination
K271 Acetylation
K271 Methylation
K271 Sumoylation
K271 Ubiquitination
K275 Sumoylation
K277 Ubiquitination
S289 Phosphorylation
T294 Phosphorylation
K308 Ubiquitination
K316 Sumoylation
K316 Ubiquitination
K324 Acetylation
K324 Ubiquitination
T328 Phosphorylation
K336 Acetylation
K336 Ubiquitination
K346 Ubiquitination
K371 Sumoylation
K409 Ubiquitination
K420 Sumoylation
K420 Ubiquitination
K427 Ubiquitination
K467 Ubiquitination
K472 Ubiquitination
S492 Phosphorylation
S493 Phosphorylation
K504 Ubiquitination
K505 Ubiquitination
S507 Phosphorylation
K540 Sumoylation
K553 Ubiquitination
K557 Ubiquitination
T578 Phosphorylation
S592 Phosphorylation
S598 Phosphorylation
K611 Sumoylation
K613 Sumoylation
K617 Acetylation
K617 Sumoylation
K617 Ubiquitination
S621 Phosphorylation
K623 Acetylation
K623 Sumoylation
K623 Ubiquitination
S625 Phosphorylation
K630 Sumoylation

Research Backgrounds

Function:

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

PTMs:

Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.

Family&Domains:

Belongs to the ubiquitin-activating E1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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