SENP5 Antibody - #AF0276

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Product: SENP5 Antibody
Catalog: AF0276
Description: Rabbit polyclonal antibody to SENP5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 86kDa; 87kD(Calculated).
Uniprot: Q96HI0
RRID: AB_2833446

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
SENP5 Antibody detects endogenous levels of total SENP5.
RRID:
AB_2833446
Cite Format: Affinity Biosciences Cat# AF0276, RRID:AB_2833446.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FKSG45; FLJ42398; MGC27076; Protease FKSG45; SENP5; SENP5_HUMAN; Sentrin specific protease SENP5; Sentrin-specific protease 5; Sentrin/SUMO specific protease SENP5; Sentrin/SUMO-specific protease SENP5; SUMO specific protease SENP5; SUMO1/sentrin specific peptidase 5; SUMO1/sentrin specific protease 5;

Immunogens

Immunogen:
Uniprot:

Q96HI0(SENP5_HUMAN) >>Visit HPA database.

Gene(ID):
SENP5(205564)
Description:
SENP5 Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates.
Sequence:
MKKQRKILWRKGIHLAFSEKWNTGFGGFKKFYFHQHLCILKAKLGRPVTWNRQLRHFQGRKKALQIQKTWIKDEPLCAKTKFNVATQNVSTLSSKVKRKDAKHFISSSKTLLRLQAEKLLSSAKNSDHEYCREKNLLKAVTDFPSNSALGQANGHRPRTDPQPSDFPMKFNGESQSPGESGTIVVTLNNHKRKGFCYGCCQGPEHHRNGGPLIPKKFQLNQHRRIKLSPLMMYEKLSMIRFRYRILRSQHFRTKSKVCKLRKAQRSWVQKVTGDHQETRRENGEGGSCSPFPSPEPKDPSCRHQPYFPDMDSSAVVKGTNSHVPDCHTKGSSFLGKELSLDEAFPDQQNGSATNAWDQSSCSSPKWECTELIHDIPLPEHRSNTMFISETEREIMTLGQENQTSSVSDDRVKLSVSGADTSVSSVDGPVSQKAVQNENSYQMEEDGSLKQSILSSELLDHPYCKSPLEAPLVCSGLKLENQVGGGKNSQKASPVDDEQLSVCLSGFLDEVMKKYGSLVPLSEKEVLGRLKDVFNEDFSNRKPFINREITNYRARHQKCNFRIFYNKHMLDMDDLATLDGQNWLNDQVINMYGELIMDAVPDKVHFFNSFFHRQLVTKGYNGVKRWTKKVDLFKKSLLLIPIHLEVHWSLITVTLSNRIISFYDSQGIHFKFCVENIRKYLLTEAREKNRPEFLQGWQTAVTKCIPQQKNDSDCGVFVLQYCKCLALEQPFQFSQEDMPRVRKRIYKELCECRLMD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Chicken
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96HI0 As Substrate

Site PTM Type Enzyme
K3 Acetylation
K6 Acetylation
K11 Acetylation
K62 Ubiquitination
K72 Ubiquitination
S106 Phosphorylation
S107 Phosphorylation
K109 Ubiquitination
S176 Phosphorylation
K216 Ubiquitination
Y243 Phosphorylation
K270 Ubiquitination
S287 Phosphorylation
S289 Phosphorylation
S293 Phosphorylation
S332 Phosphorylation
S421 Phosphorylation
S423 Phosphorylation
S424 Phosphorylation
S439 Phosphorylation
K449 Ubiquitination
S465 Phosphorylation
K477 Ubiquitination
K486 Ubiquitination
K513 Ubiquitination
K523 Ubiquitination
K530 Ubiquitination
T651 Phosphorylation
Y679 Phosphorylation
T682 Phosphorylation
K687 Ubiquitination
K746 Ubiquitination

Research Backgrounds

Function:

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CCAR2.

Family&Domains:

Belongs to the peptidase C48 family.

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For Research Use Only.
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