MARCKS Antibody - #AF6250
| Product: | MARCKS Antibody |
| Catalog: | AF6250 |
| Description: | Rabbit polyclonal antibody to MARCKS |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 36,80 kDa; 32kD(Calculated). |
| Uniprot: | P29966 |
| RRID: | AB_2835114 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6250, RRID:AB_2835114.
Fold/Unfold
80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate;
Immunogens
- P29966 MARCS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAPAADKEEPAAAGSGAASPSAAEKGEPAAAAAPEAGASPVEKEAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGAAAAAAEAGAASGEQAAAPGEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASAAACEAPSAAGPGAPPEQEAAPAEEPAAAAASSACAAPSQEAQPECSPEAPPAEAAE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P29966 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S27 | Phosphorylation | P24941 (CDK2) , P17252 (PRKCA) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| S46 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S52 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S83 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K105 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | P17252 (PRKCA) , P24941 (CDK2) | Uniprot |
| T120 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S132 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T133 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S134 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S135 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T143 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T150 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K152 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Q13464 (ROCK1) , P17252 (PRKCA) , P05771 (PRKCB) , P17612 (PRKACA) , Q16512 (PKN1) | Uniprot |
| K161 | Methylation | Uniprot | |
| S163 | Phosphorylation | P17252 (PRKCA) , Q16512 (PKN1) , P05771 (PRKCB) | Uniprot |
| K165 | Ubiquitination | Uniprot | |
| S167 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S170 | Phosphorylation | P17252 (PRKCA) , Q16512 (PKN1) , P05771 (PRKCB) | Uniprot |
| K172 | Acetylation | Uniprot | |
| K172 | Methylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K248 | Methylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S314 | Phosphorylation | Uniprot |
Research Backgrounds
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
Cytoplasm>Cytoskeleton. Membrane>Lipid-anchor.
Belongs to the MARCKS family.
Research Fields
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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