Product: TFB2M Antibody
Catalog: DF13810
Description: Rabbit polyclonal antibody to TFB2M
Application: IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 40kD; 45kD(Calculated).
Uniprot: Q9H5Q4

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
TFB2M Antibody detects endogenous levels of total TFB2M.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

N''-adenosyl(rRNA) dimethyltransferase 2; S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2; Dimethyladenosine transferase 2; dimethyladenosine transferase 2 mitochondrial; FLJ22661; FLJ23182; h mtTFB; h mtTFB2; h-mtTFB; h-mtTFB2; HCV NS5A transactivated protein 5; HCV NS5A-transactivated protein 5; hepatitis C virus NS5A transactivated protein 5; Hepatitis C virus NS5A-transactivated protein 5; Hkp1; hTFB2M; Mitochondrial 12S rRNA dimethylase 2; mitochondrial; Mitochondrial dimethyladenosine transferase 2; Mitochondrial transcription factor B2; mtTFB; mtTFB2; NS5ATP5; S-adenosylmethionine-6-N''; TFB2M; TFB2M_HUMAN; Transcription Factor B2; Transcription factor B2 mitochondrial;

Immunogens

Immunogen:

A synthesized peptide derived from Human TFB2M.

Uniprot:
Gene(ID):
Expression:
Q9H5Q4 TFB2M_HUMAN:

Ubiquitously expressed.

Sequence:
MWIPVVGLPRRLRLSALAGAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNLDGKLRVIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR

Research Backgrounds

Function:

S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Stimulates transcription independently of the methyltransferase activity.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.

Restrictive clause

 

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