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  • Product Name
    MBP Mouse monoclonal Antibody
  • Catalog No.
    BF8010
  • Source
    Mouse
  • Application
    WB,IHC
  • Reactivity
    Human, Mouse, Rat
  • UniProt
  • Mol.Wt
    33kD;
    33kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

GDB; Golli MBP; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MBP; MBP_CAVPO; MBP_HUMAN; MGC99675; MLD; Myelin A1 protein; Myelin A1 Protein, basic; Myelin basic protein; Myelin Deficient; Myelin membrane encephalitogenic protein; OTTHUMP00000163776; OTTHUMP00000174387; OTTHUMP00000174388; SHI; Shiverer; SP;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

AFB19414

Purification:

Affinity-chromatography.

Specificity:

MBP antibody detects endogenous levels of total MBP.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human MBP.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

MBP

Molecular Weight:

Observed Mol.Wt.: 33kD.
Predicted Mol.Wt.: 33kDa(Calculated)..

Tissue Specificity:

P02686 MBP_HUMAN:
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

Sequence:
MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR

Research Background

Function:

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

Post-translational Modifications:

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.

The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).

Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

Subcellular Location:

Plasma membrane>Myelin membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic side of myelin.

Nucleus.
Note: Targeted to nucleus in oligodendrocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

Subunit Structure:

Homodimer. Isoform 3 exists as a homodimer.

Similarity:

Belongs to the myelin basic protein family.

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Catalog Number :

BF8010-BP
(Blocking peptide available as BF8010-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

P02686 as Substrate
Site PTM Type Enzyme
S16 Phosphorylation
S19 Phosphorylation
S26 Phosphorylation
S36 Phosphorylation
T38 Phosphorylation
T39 Phosphorylation
S40 Phosphorylation
T65 Phosphorylation
S87 Phosphorylation
S96 Phosphorylation P27361 (MAPK3)
T106 Phosphorylation
S114 Phosphorylation
S141 Phosphorylation
S146 Phosphorylation P17612 (PRKACA)
Y148 Phosphorylation
S153 Phosphorylation
T154 Phosphorylation
T169 Phosphorylation P17612 (PRKACA)
S190 Phosphorylation P17612 (PRKACA)
Y203 Phosphorylation
S205 Phosphorylation
T229 Phosphorylation P28482 (MAPK1)
T232 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
R241 Methylation
S249 Phosphorylation P17612 (PRKACA)
Y261 Phosphorylation
S266 Phosphorylation P17612 (PRKACA)
T283 Phosphorylation
S285 Phosphorylation
S295 Phosphorylation P17612 (PRKACA)
S299 Phosphorylation Q8TAS1 (UHMK1)
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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