Nuclear factor 1 C-type Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using Nuclear factor 1 C-type Antibody.

Product:	Nuclear factor 1 C-type Antibody
Catalog:	AF0022
Description:	Rabbit polyclonal antibody to Nuclear factor 1 C-type
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Mol.Wt.:	55 KD; 56kD(Calculated).
Uniprot:	P08651

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Clonality:

Polyclonal

Specificity:

Nuclear Factor 1 C-type Antibody detects endogenous levels of total Nuclear Factor 1 C-type.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Nuclear factor 1 C-type;NFIC;NF1-C;Nuclear factor 1/C;CCAAT-box-binding transcription factor;CTF;Nuclear factor I/C;NF-I/C;NFI-C;TGGCA-binding protein;NFIC;NFI;

Immunogens

Immunogen:

A synthesized peptide derived from human Nuclear Factor 1 C-type.

Uniprot:

P08651(NFIC_HUMAN) >>Visit HPA database.

Sequence:

P08651 NFIC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSSRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPLNGSGQLKMPSHCLSAQMLAPPPPGLPRLALPPATKPATTSEGGATSPTSPSYSPPDTSPANRSFVGLGPRDPAGIYQAQSWYLG

PTMs - P08651 As Substrate

P08651

Site	PTM Type	Source
M1	Acetylation	Uniprot
S4	Phosphorylation	Uniprot
K58	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
K99	Ubiquitination	Uniprot
K112	Ubiquitination	Uniprot
T146	Phosphorylation	Uniprot
S192	Phosphorylation	Uniprot
S194	Phosphorylation	Uniprot
T237	Phosphorylation	Uniprot
T270	Phosphorylation	Uniprot
S273	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S276	Phosphorylation	Uniprot
S277	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot
K280	Acetylation	Uniprot
S284	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
T293	Phosphorylation	Uniprot
S294	Phosphorylation	Uniprot
Y299	Phosphorylation	Uniprot
T301	Phosphorylation	Uniprot
S302	Phosphorylation	Uniprot
S304	Phosphorylation	Uniprot
S305	Phosphorylation	Uniprot
T307	Phosphorylation	Uniprot
S310	Phosphorylation	Uniprot
T314	Phosphorylation	Uniprot
S322	Phosphorylation	Uniprot
S323	Phosphorylation	Uniprot
T328	Phosphorylation	Uniprot
S333	Phosphorylation	Uniprot
S337	Phosphorylation	Uniprot
S339	Phosphorylation	Uniprot
S343	Phosphorylation	Uniprot
R345	Methylation	Uniprot
T350	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
R365	Methylation	Uniprot
S366	Phosphorylation	Uniprot
S371	Phosphorylation	Uniprot
R395	Methylation	Uniprot
S427	Phosphorylation	Uniprot
S438	Phosphorylation	Uniprot
R451	Methylation	Uniprot
T472	Phosphorylation	Uniprot
S473	Phosphorylation	Uniprot
S475	Phosphorylation	Uniprot
Y476	Phosphorylation	Uniprot
S477	Phosphorylation	Uniprot
T481	Phosphorylation	Uniprot
S482	Phosphorylation	Uniprot
S487	Phosphorylation	Uniprot

Research Backgrounds

P08651_NFIC_HUMAN

Function:

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subcellular Location:

Nucleus.

Subunit Structure:

Binds DNA as a homodimer.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the CTF/NF-I family.

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Nuclear factor 1 C-type Antibody - #AF0022