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$250 50ul
$350 100ul

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  • Product Name
    SIGLEC15 Mouse Monoclonal Antibody
  • Catalog No.
    BF8008
  • Source
    Mouse
  • Application
    IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • UniProt
  • Mol.Wt
    36kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

CD33 antigen-like 3;CD33 molecule-like 3;CD33L3;HsT1361;Sialic acid-binding Ig-like lectin 15;SIG15_HUMAN;Siglec-15;SIGLEC15;

Applications:

IHC 1:50-1:200,, ELISA(peptide) 1:20000-1:40000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

AFB19405

Purification:

Affinity-chromatography.

Specificity:

SIGLEC15 Mouse Monoclonal Antibody detects endogenous levels of total SIGLEC15.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human SIGLEC15.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: 36kDa(Calculated)..

Tissue Specificity:

Q6ZMC9 SIG15_HUMAN:
Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.

Sequence:
MEKSIWLLACLAWVLPTGSFVRTKIDTTENLLNTEVHSSPAQRWSMQVPPEVSAEAGDAAVLPCTFTHPHRHYDGPLTAIWRAGEPYAGPQVFRCAAARGSELCQTALSLHGRFRLLGNPRRNDLSLRVERLALADDRRYFCRVEFAGDVHDRYESRHGVRLHVTAAPRIVNISVLPSPAHAFRALCTAEGEPPPALAWSGPALGNSLAAVRSPREGHGHLVTAELPALTHDGRYTCTAANSLGRSEASVYLFRFHGASGASTVALLLGALGFKALLLLGVLAARAARRRPEHLDTPDTPPRSQAQESNYENLSQMNPRSPPATMCSP

Research Background

Function:

Binds sialylated glycoproteins.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.

Subunit Structure:

Interacts with TYROBP and HCST.

Similarity:

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protocols & Troubleshooting

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Catalog Number :

BF8008-BP
(Blocking peptide available as BF8008-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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