MAP4 Antibody | Affinity Biosciences

1/4

Western blot analysis of extracts from various samples, using MAP4 Antibody.

AF0079 at 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P.

AF0079 at 1/100 staining Mouse brain tissue by IHC-P.

AF0079 at 1/100 staining Rat brain tissue by IHC-P.

Product:	MAP4 Antibody
Catalog:	AF0079
Description:	Rabbit polyclonal antibody to MAP4
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	121kDa,200kDa; 121kD(Calculated).
Uniprot:	P27816
RRID:	AB_2847896

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MAP4 Antibody detects endogenous levels of total MAP4.

RRID:

AB_2847896
Cite Format: Affinity Biosciences Cat# AF0079, RRID:AB_2847896.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730;

Immunogens

Immunogen:

A synthesized peptide derived from human MAP4.

Uniprot:

P27816(MAP4_HUMAN) >>Visit HPA database.

Sequence:

P27816 MAP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTGSPTEFLEEKMAYQEYPNSQNWPEDTNFCFQPEQVVDPIQTDPFKMYHDDDLADLVFPSSATADTSIFAGQNDPLKDSYGMSPCNTAVVPQGWSVEALNSPHSESFVSPEAVAEPPQPTAVPLELAKEIEMASEERPPAQALEIMMGLKTTDMAPSKETEMALAKDMALATKTEVALAKDMESPTKLDVTLAKDMQPSMESDMALVKDMELPTEKEVALVKDVRWPTETDVSSAKNVVLPTETEVAPAKDVTLLKETERASPIKMDLAPSKDMGPPKENKKETERASPIKMDLAPSKDMGPPKENKIVPAKDLVLLSEIEVAQANDIISSTEISSAEKVALSSETEVALARDMTLPPETNVILTKDKALPLEAEVAPVKDMAQLPETEIAPAKDVAPSTVKEVGLLKDMSPLSETEMALGKDVTPPPETEVVLIKNVCLPPEMEVALTEDQVPALKTEAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGTGKKCSLPAEEDSVLEKLGERKPCNSQPSELSSETSGIARPEEGRPVVSGTGNDITTPPNKELPPSPEKKTKPLATTQPAKTSTSKAKTQPTSLPKQPAPTTIGGLNKKPMSLASGLVPAAPPKRPAVASARPSILPSKDVKPKPIADAKAPEKRASPSKPASAPASRSGSKSTQTVAKTTTAAAVASTGPSSRSPSTLLPKKPTAIKTEGKPAEVKKMTAKSVPADLSRPKSTSTSSMKKTTTLSGTAPAAGVVPSRVKATPMPSRPSTTPFIDKKPTSAKPSSTTPRLSRLATNTSAPDLKNVRSKVGSTENIKHQPGGGRAKVEKKTEAAATTRKPESNAVTKTAGPIASAQKQPAGKVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANIKHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEAPLCPGPPAGEEPAISEAAPEAGAPTSASGLNGHPTLSGGGDQREAQTLDSQIQETSI

PTMs - P27816 As Substrate

P27816

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
T11	Phosphorylation		Uniprot
S14	Phosphorylation		Uniprot
T28	Phosphorylation		Uniprot
T41	Phosphorylation		Uniprot
T45	Phosphorylation		Uniprot
Y47	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
T57	Phosphorylation		Uniprot
S60	Phosphorylation		Uniprot
S68	Phosphorylation		Uniprot
S71	Phosphorylation		Uniprot
T76	Phosphorylation		Uniprot
S94	Phosphorylation		Uniprot
T96	Phosphorylation		Uniprot
T97	Phosphorylation		Uniprot
S99	Phosphorylation		Uniprot
T101	Phosphorylation		Uniprot
Y110	Phosphorylation		Uniprot
Y113	Phosphorylation		Uniprot
S116	Phosphorylation		Uniprot
T183	Phosphorylation		Uniprot
S197	Phosphorylation		Uniprot
S200	Phosphorylation		Uniprot
S202	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
K254	Ubiquitination		Uniprot
T256	Phosphorylation		Uniprot
K262	Acetylation		Uniprot
K262	Ubiquitination		Uniprot
K269	Acetylation		Uniprot
K269	Sumoylation		Uniprot
K269	Ubiquitination		Uniprot
K276	Acetylation		Uniprot
S280	Phosphorylation		Uniprot
T282	Phosphorylation		Uniprot
T287	Phosphorylation		Uniprot
S295	Phosphorylation		Uniprot
K312	Ubiquitination		Uniprot
K318	Ubiquitination		Uniprot
T324	Phosphorylation		Uniprot
T326	Phosphorylation		Uniprot
S329	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
K332	Ubiquitination		Uniprot
T340	Phosphorylation		Uniprot
K346	Acetylation		Uniprot
K346	Ubiquitination		Uniprot
K352	Ubiquitination		Uniprot
T354	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
K361	Ubiquitination		Uniprot
S367	Phosphorylation		Uniprot
K368	Acetylation		Uniprot
K368	Ubiquitination		Uniprot
T380	Phosphorylation		Uniprot
S384	Phosphorylation		Uniprot
K387	Ubiquitination		Uniprot
S393	Phosphorylation		Uniprot
K394	Acetylation		Uniprot
K394	Ubiquitination		Uniprot
K400	Ubiquitination		Uniprot
S439	Phosphorylation		Uniprot
S440	Phosphorylation		Uniprot
T442	Phosphorylation		Uniprot
T451	Phosphorylation		Uniprot
K464	Ubiquitination		Uniprot
K476	Acetylation		Uniprot
K476	Ubiquitination		Uniprot
T484	Phosphorylation		Uniprot
K490	Ubiquitination		Uniprot
S495	Phosphorylation		Uniprot
T496	Phosphorylation		Uniprot
K498	Ubiquitination		Uniprot
S507	Phosphorylation		Uniprot
S510	Phosphorylation		Uniprot
T512	Phosphorylation		Uniprot
T521	Phosphorylation		Uniprot
T526	Phosphorylation		Uniprot
K560	Ubiquitination		Uniprot
T565	Phosphorylation		Uniprot
T571	Phosphorylation		Uniprot
K574	Ubiquitination		Uniprot
S580	Phosphorylation		Uniprot
T582	Phosphorylation		Uniprot
T585	Phosphorylation		Uniprot
S602	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
S609	Phosphorylation		Uniprot
T620	Phosphorylation		Uniprot
S624	Phosphorylation		Uniprot
T627	Phosphorylation		Uniprot
K633	Acetylation		Uniprot
K634	Ubiquitination		Uniprot
C635	S-Nitrosylation		Uniprot
S636	Phosphorylation		Uniprot
S643	Phosphorylation		Uniprot
K647	Ubiquitination		Uniprot
T686	Phosphorylation		Uniprot
T687	Phosphorylation		Uniprot
S696	Phosphorylation	P06493 (CDK1)	Uniprot
K702	Acetylation		Uniprot
K702	Ubiquitination		Uniprot
T706	Phosphorylation		Uniprot
T712	Phosphorylation		Uniprot
S713	Phosphorylation		Uniprot
K718	Acetylation		Uniprot
T719	Phosphorylation		Uniprot
T722	Phosphorylation		Uniprot
S723	Phosphorylation		Uniprot
K726	Ubiquitination		Uniprot
T731	Phosphorylation		Uniprot
S742	Phosphorylation		Uniprot
S745	Phosphorylation		Uniprot
S760	Phosphorylation		Uniprot
S764	Phosphorylation		Uniprot
S768	Phosphorylation		Uniprot
K784	Acetylation		Uniprot
S787	Phosphorylation	P06493 (CDK1)	Uniprot
S789	Phosphorylation		Uniprot
S793	Phosphorylation		Uniprot
S797	O-Glycosylation		Uniprot
S797	Phosphorylation		Uniprot
S803	Phosphorylation		Uniprot
T804	Phosphorylation		Uniprot
T806	Phosphorylation		Uniprot
S818	Phosphorylation		Uniprot
T819	Phosphorylation		Uniprot
S822	Phosphorylation		Uniprot
S823	Phosphorylation		Uniprot
S825	Phosphorylation		Uniprot
S827	Phosphorylation		Uniprot
T828	Phosphorylation		Uniprot
K838	Acetylation		Uniprot
K838	Sumoylation		Uniprot
K847	Acetylation		Uniprot
K852	Acetylation		Uniprot
S853	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
S863	Phosphorylation		Uniprot
S865	Phosphorylation		Uniprot
S868	Phosphorylation		Uniprot
K871	Acetylation		Uniprot
T872	Phosphorylation		Uniprot
T874	Phosphorylation		Uniprot
S876	Phosphorylation		Uniprot
T878	Phosphorylation		Uniprot
T892	Phosphorylation	P06493 (CDK1)	Uniprot
S896	Phosphorylation		Uniprot
S899	Phosphorylation		Uniprot
T900	Phosphorylation		Uniprot
T901	Phosphorylation	P06493 (CDK1)	Uniprot
T909	Phosphorylation		Uniprot
K912	Acetylation		Uniprot
S914	Phosphorylation		Uniprot
S915	Phosphorylation		Uniprot
T917	Phosphorylation	P06493 (CDK1)	Uniprot
T925	Phosphorylation	O14757 (CHEK1)	Uniprot
T927	Phosphorylation		Uniprot
S928	Phosphorylation		Uniprot
K933	Acetylation		Uniprot
K933	Ubiquitination		Uniprot
S937	Phosphorylation		Uniprot
K938	Acetylation		Uniprot
S941	Phosphorylation	Q9P0L2 (MARK1)	Uniprot
T942	Phosphorylation		Uniprot
K946	Ubiquitination		Uniprot
K955	Methylation		Uniprot
T960	Phosphorylation		Uniprot
T965	Phosphorylation		Uniprot
T977	Phosphorylation		Uniprot
S983	Phosphorylation		Uniprot
K986	Ubiquitination		Uniprot
K991	Acetylation		Uniprot
K991	Ubiquitination		Uniprot
S996	Phosphorylation		Uniprot
K997	Acetylation		Uniprot
K998	Acetylation		Uniprot
K998	Ubiquitination		Uniprot
S1000	Phosphorylation		Uniprot
Y1001	Phosphorylation		Uniprot
S1002	Phosphorylation		Uniprot
S1006	Phosphorylation		Uniprot
K1007	Ubiquitination		Uniprot
S1010	Phosphorylation		Uniprot
K1011	Methylation		Uniprot
K1015	Ubiquitination		Uniprot
K1028	Acetylation		Uniprot
K1028	Ubiquitination		Uniprot
K1034	Acetylation		Uniprot
S1036	Phosphorylation		Uniprot
S1037	Phosphorylation		Uniprot
K1038	Acetylation		Uniprot
S1041	Phosphorylation		Uniprot
K1042	Acetylation		Uniprot
S1058	Phosphorylation		Uniprot
K1060	Acetylation		Uniprot
K1060	Ubiquitination		Uniprot
K1064	Methylation		Uniprot
K1070	Acetylation		Uniprot
K1070	Ubiquitination		Uniprot
S1073	Phosphorylation	Q9P0L2 (MARK1)	Uniprot
T1142	Phosphorylation		Uniprot
S1145	Phosphorylation		Uniprot
T1150	Phosphorylation		Uniprot
S1151	Phosphorylation		Uniprot

Research Backgrounds

P27816_MAP4_HUMAN

Function:

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

PTMs:

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Subunit Structure:

Interacts with SEPTIN2; this interaction impedes tubulin-binding. Interacts with TRAF3IP1 (By similarity).

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MAP4 Antibody - #AF0079