Phospho-Phospholamban (Ser16) Antibody
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$280 100ul
$350 200ul

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  • Product Name
    Phospho-Phospholamban (Ser16) Antibody
  • Catalog No.
    AF7458
  • Source
    Rabbit
  • Application
    ELISA
  • Reactivity
    Human, Mouse, Rat
  • UniProt
  • Mol.Wt.
    24kDa,7kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

Cardiac phospholamban; CMD1P; CMH18; PLB; Pln; PPLA_HUMAN;

Applications:

, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-Phospholamban (Ser16) Antibody detects endogenous levels of Phospholamban only when phosphorylated at Ser16.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Phospholamban around the phosphorylation site of Ser16.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

P26678

Molecular Weight:

Observed Mol.Wt.: 24kD.
Predicted Mol.Wt.: 7kDa.

Tissue Specificity:

Heart muscle (at protein level).

Sequence:
        10         20         30         40         50
MEKVQYLTRS AIRRASTIEM PQQARQKLQN LFINFCLILI CLLLICIIVM

LL

Background

Function:

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.

Post-translational Modifications:

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.Palmitoylated by ZDHHC16, promoting formation of the homopentamer.

Subcellular Location:

Endoplasmic reticulum;Mitochondrion;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Homopentamer (PubMed:16043693, PubMed:16897780). Interacts with HAX1 and ATP2A2 (PubMed:17241641).

Similarity:

Belongs to the phospholamban family.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway.(View pathway)
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.(View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.(View pathway)

Protocols & Troubleshooting

Not available

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Catalog Number :

AF7458-BP
(Blocking peptide available as AF7458-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

P26678 as Substrate
Site PTM Type Enzyme
S16 Phosphorylation Q09013 (DMPK) , P17612 (PRKACA) , P31749 (AKT1)
T17 Phosphorylation Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , Q13554 (CAMK2B)
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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