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  • Product Name
    IRF3 Antibody
  • Catalog No.
    AF2736
  • RRID
    AB_2847772
  • Source
    Rabbit
  • Application
    WB,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(%), Bovine(%), Horse(%), Sheep(%)
  • UniProt
  • Mol.Wt
    (Observed)45-55kD; (Calculated)47kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

IIAE7; Interferon regulatory factor 3; IRF 3; IRF-3; IRF3; IRF3_HUMAN; MGC94729;

Applications:

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(%), Bovine(%), Horse(%), Sheep(%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin(Thermo Fisher Scientific).

Specificity:

IRF3 Antibody detects endogenous levels of total IRF3.

RRID:

AB_2847772
Please cite this product as: Affinity Biosciences Cat# AF2736, RRID:AB_2847772.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human IRF3.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

IRF3

Molecular Weight:

Observed Mol.Wt.: (Observed)45-55kD.
Predicted Mol.Wt.: (Calculated)47kDa.

Tissue Specificity:

Q14653 IRF3_HUMAN:
Expressed constitutively in a variety of tissues.

Sequence:
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES

Research Background

Function:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591).

Post-translational Modifications:

Constitutively phosphorylated on many Ser/Thr residues (PubMed:22394562, PubMed:23478265, PubMed:23746807). Activated following phosphorylation by TBK1 and IKBKE (PubMed:23478265, PubMed:23746807, PubMed:25636800). Innate adapter protein MAVS, STING1 or TICAM1 are first activated by viral RNA, cytosolic DNA, and bacterial lipopolysaccharide (LPS), respectively, leading to activation of the kinases TBK1 and IKBKE (PubMed:25636800). These kinases then phosphorylate the adapter proteins on the pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (PubMed:25636800). Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce IFNs (PubMed:25636800).

(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.

Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.

ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757). When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Expressed constitutively in a variety of tissues.

Subunit Structure:

Monomer (PubMed:16846591, PubMed:16979567, PubMed:20049431). Homodimer; phosphorylation-induced (PubMed:22394562, PubMed:25636800, PubMed:26347139). Interacts (when phosphorylated) with CREBBP (PubMed:16154084, PubMed:27302953). Interacts with MAVS (via phosphorylated pLxIS motif) (PubMed:16153868, PubMed:25636800, PubMed:27302953). Interacts with TICAM1 (via phosphorylated pLxIS motif) (PubMed:12471095, PubMed:14739303, PubMed:25636800, PubMed:27302953). Interacts with STING1 (via phosphorylated pLxIS motif) (PubMed:22394562, PubMed:25636800, PubMed:27302953). Interacts with IKBKE and TBK1 (PubMed:16281057, PubMed:23478265, PubMed:25636800). Interacts with TICAM2 (PubMed:14517278). Interacts with RBCK1 (PubMed:18711448). Interacts with HERC5 (PubMed:20308324). Interacts with DDX3X (phosphorylated at 'Ser-102'); the interaction allows the phosphorylation and activation of IRF3 by IKBKE (PubMed:23478265). Interacts with TRIM21 and ULK1, in the presence of TRIM21; this interaction leads to IRF3 degradation by autophagy (PubMed:18641315, PubMed:26347139). Interacts with RIOK3; RIOK3 probably mediates the interaction of TBK1 with IRF3 (PubMed:19557502). Interacts with ILRUN; the interaction inhibits IRF3 binding to its DNA consensus sequence (PubMed:29802199). Interacts with LYAR; this interaction impairs IRF3 DNA-binding activity (PubMed:31413131).

(Microbial infection) Interacts with rotavirus A NSP1 (via pLxIS motif); this interaction leads to the proteasome-dependent degradation of IRF3.

(Microbial infection) Interacts with herpes virus 8/HHV-8 protein VIRF1 (PubMed:11314014).

(Microbial infection) Interacts with Seneca Valley virus protease 3C; this interaction is involved in the suppression of IRF3 expression and phosphorylation by the virus.

Similarity:

Belongs to the IRF family.

Research Fields

Research Fields:

· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.(View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)

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Catalog Number :

AF2736-BP
(Blocking peptide available as AF2736-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Q14653 as Substrate
Site PTM Type Enzyme
T3 Phosphorylation
S14 Phosphorylation
K29 Ubiquitination
K70 Sumoylation
K70 Ubiquitination
T75 Phosphorylation
K77 Ubiquitination
K87 Sumoylation
K87 Ubiquitination
S97 Phosphorylation
S123 Phosphorylation
T135 Phosphorylation P78527 (PRKDC)
S173 Phosphorylation P45984 (MAPK9) , P45983 (MAPK8) , Q9UHD2 (TBK1)
S175 Phosphorylation Q9UHD2 (TBK1)
T180 Phosphorylation
S188 Phosphorylation
K193 Ubiquitination
T237 Phosphorylation
T244 Phosphorylation
T253 Phosphorylation
S259 Phosphorylation
K313 Ubiquitination
K315 Ubiquitination
S339 Phosphorylation
T370 Phosphorylation
S385 Phosphorylation Q9UHD2 (TBK1)
S386 Phosphorylation Q14164 (IKBKE) , Q9UQM7 (CAMK2A) , Q9UHD2 (TBK1)
T390 Phosphorylation
S396 Phosphorylation O14920 (IKBKB) , Q14164 (IKBKE) , Q9UHD2 (TBK1)
S398 Phosphorylation O14920 (IKBKB) , Q14164 (IKBKE) , Q9UHD2 (TBK1)
S402 Phosphorylation O14920 (IKBKB) , Q14164 (IKBKE) , Q9UHD2 (TBK1)
T404 Phosphorylation O14920 (IKBKB) , Q9UHD2 (TBK1)
S405 Phosphorylation O14920 (IKBKB) , Q9UHD2 (TBK1)
S427 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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