Thrombin Receptor Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using Thrombin Receptor Antibody.

Western blot analysis of extracts from Mouse brain, using Thrombin Receptor Antibody.

Western blot analysis of extracts from Rat brain, using Thrombin Receptor Antibody.

AF0263 at 1/100 staining Rat kidney tissue by IHC-P.

AF0263 staining HepG2 by IF/ICC.

(G) Confocal immunoﬂuorescence imaging was performed to visualize PAR1 and doxycycline.

Fig.

Product:	Thrombin Receptor Antibody
Catalog:	AF0263
Description:	Rabbit polyclonal antibody to Thrombin Receptor
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	48kDa; 47kD(Calculated).
Uniprot:	P25116
RRID:	AB_2833437

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Thrombin Receptor Antibody detects endogenous levels of total Thrombin Receptor.

RRID:

AB_2833437
Cite Format: Affinity Biosciences Cat# AF0263, RRID:AB_2833437.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CF2R; Coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR 1; PAR-1; PAR1; PAR1_HUMAN; Protease activated receptor 1; Proteinase activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; TR;

Immunogens

Immunogen:

Uniprot:

P25116(PAR1_HUMAN) >>Visit HPA database.

Gene(ID):

F2R(2149)

Expression:

P25116 PAR1_HUMAN:

Platelets and vascular endothelial cells.

Description:

PAR1 a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.

Sequence:

P25116 PAR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPRRLLLVAACFSLCGPLLSARTRARRPESKATNATLDPRSFLLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLTLFVPSVYTGVFVVSLPLNIMAIVVFILKMKVKKPAVVYMLHLATADVLFVSVLPFKISYYFSGSDWQFGSELCRFVTAAFYCNMYASILLMTVISIDRFLAVVYPMQSLSWRTLGRASFTCLAIWALAIAGVVPLLLKEQTIQVPGLNITTCHDVLNETLLEGYYAYYFSAFSAVFFFVPLIISTVCYVSIIRCLSSSAVANRSKKSRALFLSAAVFCIFIICFGPTNVLLIAHYSFLSHTSTTEAAYFAYLLCVCVSSISCCIDPLIYYYASSECQRYVYSILCCKESSDPSSYNSSGQLMASKMDTCSSNLNNSIYKKLLT

PTMs - P25116 As Substrate

P25116

Site	PTM Type	Source
K51	Ubiquitination	Uniprot
Y52	Phosphorylation	Uniprot
Y69	Phosphorylation	Uniprot
S306	Phosphorylation	Uniprot
S375	Phosphorylation	Uniprot
S376	Phosphorylation	Uniprot
Y383	Phosphorylation	Uniprot
S384	Phosphorylation	Uniprot
S391	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot
S395	Phosphorylation	Uniprot
S396	Phosphorylation	Uniprot
Y397	Phosphorylation	Uniprot
S399	Phosphorylation	Uniprot
S400	Phosphorylation	Uniprot
S406	Phosphorylation	Uniprot
K407	Ubiquitination	Uniprot
T410	Phosphorylation	Uniprot
S412	Phosphorylation	Uniprot
S413	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
Y420	Phosphorylation	Uniprot
K421	Ubiquitination	Uniprot
K422	Ubiquitination	Uniprot
T425	Phosphorylation	Uniprot

Research Backgrounds

P25116_PAR1_HUMAN

Function:

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

PTMs:

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Phosphorylated in the C-terminal tail; probably mediating desensitization prior to the uncoupling and internalization of the receptor.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Platelets and vascular endothelial cells.

Family&Domains:

The cleaved signal peptide may not be degraded and may function as an intracellular angiogenesis inhibitor peptide known as parstatin.

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

References

1). Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia. MOLECULAR HUMAN REPRODUCTION (PubMed: 29325127) [IF=4.0]

2). pHLIP(Var7)-P1AP suppresses tumor cell proliferation in MDA-MB-231 triple-negative breast cancer by targeting protease activated receptor 1. BREAST CANCER RESEARCH AND TREATMENT (PubMed: 32034579) [IF=3.8]

Application: IF/ICC Species: Human Sample: MDA-MB-231 and MCF-10A cells

Fig. 1 PAR1 expression was positive in MDA-MB-231 cells, and PAR1 expression was negative in MCF-10A cells (400 ×)

3). Doxycycline directly targets PAR1 to suppress tumor progression. Oncotarget (PubMed: 28187433)

Application: IF/ICC Species: human Sample:

(G) Confocal immunoﬂuorescence imaging was performed to visualize PAR1 and doxycycline. PAR1 and doxycycline co-localize, with a Pearson’s correlation coeffcient of 0.944 and a Mander’s overlap of 0.964.

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Thrombin Receptor Antibody - #AF0263