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BCL-2 Antibody AF6139

  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
  • BCL-2 Antibody
BCL-2 Antibody
  • Catalog No.
    AF6139
  • Source
    Rabbit
  • P10415 UniProt
  • 596 Gene ID
  • Predicted M.W.
    27kDa
  • Observed M.W.
    26kDa
  • Concentration
    1mg/ml
  • Clonality
    Polyclonal
  • Price/Size
    280$/100ul,350$/200ul
  • Delivery time
    Same day delivery

Browsing history

Datasheet Validation(9) Citations(20) Review Blocking Peptides

1). Chen Y et al. Iridoid glycoside from Cornus officinalis ameliorated diabetes mellitus-induced testicular damage in male rats: Involvement of suppression of the AGEs/RAGE/p38 MAPK signaling pathway. J Ethnopharmacol 2016 Dec 24;194:850-860 (PubMed: 27989876)

Application: WB;   Species: rat;    Sample:None Available;

WB


2). Liu Y et al. Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells. Neurochem Res 2016 Oct;41(10):2728-2751 (PubMed: 27394418)

Application: WB;   Species: human;    Sample: u87;

WB


3). Zhao ZH et al. PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury. Cell Mol Neurobiol 2017 May;37(4):707-715 (PubMed: 27443384)

Application: WB(1/3000);   Species: mouse;    Sample:None Available;

WB


4). Li R et al. Hyperlipidemia impairs erectile function in rats by causing cavernosal fibrosis. Andrologia 2017 Sep;49(7) (PubMed: 27619893)

Application: WB(1/500);   Species: rat;    Sample:None Available;

WB


5). Wang H et al. Baicalin extracted from Huangqin (Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma (Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9. J Tradit Chin Med 2017 Apr;37(2):229-5 (PubMed: 29960296)

Application: None Available;   Species:None Available;    Sample:None Available;



6). Liu QQ et al. Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats. Oncotarget 2017 Jun 20;8(32):53465-53481 (PubMed: 28881824)

Application: WB;   Species: rat;    Sample:None Available;

WB


7). Wang F et al. Angelicin inhibits liver cancer growth in vitro and in vivo. Mol Med Rep 2017 Oct;16(4):5441-5449 (PubMed: 28849216)

Application: WB;   Species: human;    Sample:None Available;

WB


8). Minfei Yang et al. Estrogen receptor β exhibited anti-tumor effects on osteosarcoma cells by regulating integrin, IAP, NFkB/BCL-2 and PI3K/Akt signal pathway. J BONE ONCOL 2017 Nov;9:15-20

Application: None Available;   Species:None Available;    Sample:None Available;



9). Li H et al. The effect of cavernous nerve traction on erectile function in rats. PLoS One 2017 Oct 5;12(10):e0186077 (PubMed: 28982169)

Application: WB(1/1000);   Species: rat;    Sample:None Available;

WB


10). Mengyang Zhao et al. Packaging cordycepin phycocyanin micelles for the inhibition of brain cancer. J. Mater. Chem. B 2017;5:6016-6026

Application: None Available;   Species:None Available;    Sample:None Available;



11). Pan L et al. The cardiac glycoside oleandrin induces apoptosis in human colon cancer cells via the mitochondrial pathway. Cancer Chemother Pharmacol 2017 Jul;80(1):91-100 (PubMed: 28597038)

Application: WB(1/1000);   Species: human;    Sample: SW480;

WB


12). Zhou Y et al. Altered Light Conditions Contribute to Abnormalities in Emotion and Cognition Through HINT1 Dysfunction in C57BL/6 Mice. Front Behav Neurosci 2018 Jun 8;12:110 (PubMed: 29937721)

Application: None Available;   Species:None Available;    Sample:None Available;



13). Lu Q et al. The mTOR promotes oxidative stress-induced apoptosis of mesangial cells in diabetic nephropathy. Mol Cell Endocrinol 2018 Sep 15;473:31-43 (PubMed: 29277549)

Application: None Available;   Species:None Available;    Sample:None Available;



14). Gao Y et al. Betulinic acid induces apoptosis and ultrastructural changes in MDA-MB-231 breast cancer cells. Ultrastruct Pathol 2018 Jan-Feb;42(1):49-54 (PubMed: 29192840)

Application: None Available;   Species:None Available;    Sample:None Available;



15). Shao Q et al. MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition. Gene 2018 Apr 30;652:48-58 (PubMed: 29427737)

Application: None Available;   Species:None Available;    Sample:None Available;



16). Wang W et al. Mitofusin-2 Triggers Cervical Carcinoma Cell Hela Apoptosis via Mitochondrial Pathway in Mouse Model. Cell Physiol Biochem 2018;46(1):69-81 (PubMed: 29587277)

Application: None Available;   Species:None Available;    Sample:None Available;



17). Liu Y et al. Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway. Mar Drugs 2018 Mar 6;16(3) (PubMed: 29509717)

Application: None Available;   Species:None Available;    Sample:None Available;



18). Yin L et al. Bacillus spore-based oral carriers loading curcumin for the therapy of colon cancer. J Control Release 2018 Feb 10;271:31-44 (PubMed: 29274436)

Application: None Available;   Species:None Available;    Sample:None Available;



19). Zhang Y et al. Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase. Oncotarget 2016 Jun 28;7(26):39302-39315 (PubMed: 27283992)

Application: WB(1/1000);   Species: rat;    Sample:None Available;

WB


20). Yang M et al. Estrogen receptor β exhibited anti-tumor effects on osteosarcoma cells by regulating integrin, IAP, NF-kB/BCL-2 and PI3K/Akt signal pathway. J Bone Oncol 2017 Sep 28;9:15-20 (PubMed: 29071206)

Application: WB(1/2000);   Species: human;    Sample:None Available;

WB


Expand↓

Alternative Names:

Apoptosis regulator Bcl 2; Apoptosis regulator Bcl-2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; Bcl-2; Bcl2; BCL2_HUMAN; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; PPP1R50; Protein phosphatase 1, regulatory subunit 50;

Applications:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Source:

Rabbit

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Concentration:

1mg/ml

Molecular Weight:

Observed: 26kDa. Predicted: 27kDa.

BCL-2 Antibody Western blot analysis of BCL-2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Western blot analysis of BCL-2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
BCL-2 Antibody This image is a courtesy of anonymous review. This image is a courtesy of anonymous review.
BCL-2 Antibody Western blot analysis of extracts of various sample,using bcl2 antibody. Western blot analysis of extracts of various sample,using bcl2 antibody.
BCL-2 Antibody AF6139 at 1/100 dilution staining bcl2 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue AF6139 at 1/100 dilution staining bcl2 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue
BCL-2 Antibody AF6139 staining A549 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A  Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody. AF6139 staining A549 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
BCL-2 Antibody Western blot analysis of extracts from Mouse liver, using BCL-2 Antibody. Lane 1 was treated with the blocking peptide. Western blot analysis of extracts from Mouse liver, using BCL-2 Antibody. Lane 1 was treated with the blocking peptide.
BCL-2 Antibody AF6139 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody. AF6139 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BCL-2 Antibody Western blot analysis of extracts from rat brain, mouse brain, mouse muscle, rat liver, using BCL-2 Antibody. Western blot analysis of extracts from rat brain, mouse brain, mouse muscle, rat liver, using BCL-2 Antibody.
BCL-2 Antibody Western blot analysis of extracts from various samples, using BCL-2 Antibody. 
Lane 1: 293 cells treated with the blocking peptide.
Lane 2: 293 cells;
Lane 3: mouse brain tissue;
Lane 4: HuvEc cells. Western blot analysis of extracts from various samples, using BCL-2 Antibody. Lane 1: 293 cells treated with the blocking peptide. Lane 2: 293 cells; Lane 3: mouse brain tissue; Lane 4: HuvEc cells.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

BCL-2 Antibody detects endogenous levels of total BCL-2.

Immunogen:

A synthesized peptide derived from human BCL-2.

Uniprot:

Gene id:

Show more↓

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species.(View pathway)
· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.(View pathway)

Description:

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Function:

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Subcellular Location:

Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.

Post-translational Modifications:

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Tissue Specificity:

Expressed in a variety of tissues.

Subunit Structure:

Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:8183370). Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex. Interacts with RTL10/BOP. Interacts with the SCF(FBXO10) complex. Interacts (via the loop between motifs BH4 and BH3) with NLRP1 (via LRR repeats), but not with NLRP2, NLRP3, NLRP4, PYCARD, nor MEFV (PubMed:17418785).

Similarity:

BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.Belongs to the Bcl-2 family.

Protein BLAST With

Sequence:

        10         20         30         40         50
MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS
60 70 80 90 100
SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA
110 120 130 140 150
GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF
160 170 180 190 200
FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE
210 220 230
LYGPSMRPLF DFSWLSLKTL LSLALVGACI TLGAYLGHK

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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Catalog Number :

AF6139-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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