Phospho-LETM1 (Thr192) Antibody | Affinity Biosciences

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Western blot analysis of extracts from P19 cells(heat shock treatment), using Phospho-LETM1 (Thr192) Antibody.

Product:	Phospho-LETM1 (Thr192) Antibody
Catalog:	AF3759
Description:	Rabbit polyclonal antibody to Phospho-LETM1 (Thr192)
Application:	WB
Reactivity:	Human, Mouse
Mol.Wt.:	83kD(Calculated).
Uniprot:	O95202
RRID:	AB_2847073

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-LETM1 (Thr192) Antibody detects endogenous levels of LETM1 only when phosphorylated at Thr192.

RRID:

AB_2847073
Cite Format: Affinity Biosciences Cat# AF3759, RRID:AB_2847073.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

LETM 1; LETM1 and EF hand domain containing protein 1, mitochondrial; LETM1 and EF-hand domain-containing protein 1; Letm1; LETM1_HUMAN; Leucine zipper EF hand containing transmembrane protein 1; Leucine zipper-EF-hand-containing transmembrane protein 1; Mdm38 homolog; mitochondrial;

Immunogens

Immunogen:

A synthesized peptide derived from human LETM1 around the phosphorylation site of Thr192.

Uniprot:

O95202(LETM1_HUMAN) >>Visit HPA database.

Gene(ID):

LETM1(3954)

Sequence:

O95202 LETM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAHLSCASTLGLRNCLNVPFGCCTPIHPVYTSSRGDHLGCWALRPECLRIVSRAPWTSTSVGFVAVGPQCLPVRGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPAEVVVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILNGHSLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSLKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGSATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTEDRLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS

PTMs - O95202 As Substrate

O95202

Site	PTM Type	Source
S3	Phosphorylation	Uniprot
S8	Phosphorylation	Uniprot
S126	Phosphorylation	Uniprot
Y141	Phosphorylation	Uniprot
S142	Phosphorylation	Uniprot
Y164	Phosphorylation	Uniprot
T175	Phosphorylation	Uniprot
S190	Phosphorylation	Uniprot
T192	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
K257	Ubiquitination	Uniprot
K284	Acetylation	Uniprot
S287	Phosphorylation	Uniprot
K292	Ubiquitination	Uniprot
R307	Methylation	Uniprot
K373	Ubiquitination	Uniprot
R393	Methylation	Uniprot
Y579	Phosphorylation	Uniprot
S580	Phosphorylation	Uniprot
K597	Acetylation	Uniprot
S602	Phosphorylation	Uniprot
T643	Phosphorylation	Uniprot
S649	Phosphorylation	Uniprot
S666	Phosphorylation	Uniprot
K682	Ubiquitination	Uniprot
S739	Phosphorylation	Uniprot

Research Backgrounds

O95202_LETM1_HUMAN

Function:

Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion. Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein.

Subunit Structure:

Homohexamer (By similarity). Can form 2 complexes: a major (300 kDa) and a minor complex (500-600 kDa). Interacts with BCS1L.

Family&Domains:

Belongs to the LETM1 family.

Restrictive clause

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Phospho-LETM1 (Thr192) Antibody - #AF3759