gamma Synuclein Antibody | Affinity Biosciences

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AF0258 at 1/100 staining human breast carcinoma tissue sections by IHC-P.

Product:	gamma Synuclein Antibody
Catalog:	AF0258
Description:	Rabbit polyclonal antibody to gamma Synuclein
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	20kDa; 13kD(Calculated).
Uniprot:	O76070
RRID:	AB_2833433

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

gamma Synuclein Antibody detects endogenous levels of total gamma Synuclein.

RRID:

AB_2833433
Cite Format: Affinity Biosciences Cat# AF0258, RRID:AB_2833433.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BCSG1; Breast cancer specific gene 1 protein; Breast cancer specific protein 1; Breast cancer-specific gene 1 protein; Gamma synuclein; Gamma-synuclein; Persyn; PRSN; SNCG; SR; Synoretin; Synuclein gamma; synuclein, gamma (breast cancer-specific protein 1); SYUG_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human gamma Synuclein, corresponding to a region within C-terminal amino acids.

Uniprot:

O76070(SYUG_HUMAN) >>Visit HPA database.

Gene(ID):

SNCG(6623)

Expression:

O76070 SYUG_HUMAN:

Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.

Description:

SNCG a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. May be involved in modulating axonal architecture during development and in the adult. Plays a role in neurofilament network integrity. High levels have been identified in advanced breast carcinomas.

Sequence:

O76070 SYUG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVFKKGFSIAKEGVVGAVEKTKQGVTEAAEKTKEGVMYVGAKTKENVVQSVTSVAEKTKEQANAVSEAVVSSVNTVATKTVEEAENIAVTSGVVRKEDLRPSAPQQEGEASKEKEEVAEEAQSGGD

PTMs - O76070 As Substrate

O76070

Site	PTM Type	Enzyme	Source
K5	Methylation		Uniprot
K6	Methylation		Uniprot
S9	Phosphorylation		Uniprot
Y39	Phosphorylation		Uniprot
T44	Phosphorylation		Uniprot
S51	Phosphorylation		Uniprot
S54	Phosphorylation		Uniprot
K58	Acetylation		Uniprot
S112	Phosphorylation		Uniprot
S124	Phosphorylation	P34947 (GRK5)	Uniprot

Research Backgrounds

O76070_SYUG_HUMAN

Function:

Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway (By similarity).

PTMs:

Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.

Subcellular Location:

Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.

Tissue Specificity:

Subunit Structure:

May be a centrosome-associated protein. Interacts with MYOC; affects its secretion and its aggregation (By similarity).

Family&Domains:

Belongs to the synuclein family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

gamma Synuclein Antibody - #AF0258