AS160 Antibody | Affinity Biosciences

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Western blot analysis of extracts from EC304 cells, using AS160 Antibody.

Product:	AS160 Antibody
Catalog:	AF2617
Description:	Rabbit polyclonal antibody to AS160
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	160kDa; 147kD(Calculated).
Uniprot:	O60343
RRID:	AB_2846800

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

AS160 Antibody detects endogenous levels of total AS160.

RRID:

AB_2846800
Cite Format: Affinity Biosciences Cat# AF2617, RRID:AB_2846800.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;

Immunogens

Immunogen:

A synthesized peptide derived from human AS160.

Uniprot:

O60343(TBCD4_HUMAN) >>Visit HPA database.

Gene(ID):

TBC1D4(9882)

Expression:

O60343 TBCD4_HUMAN:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

Sequence:

O60343 TBCD4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP

PTMs - O60343 As Substrate

O60343

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S44	Phosphorylation		Uniprot
S66	Phosphorylation		Uniprot
K68	Ubiquitination		Uniprot
S102	Phosphorylation		Uniprot
K228	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
S262	Phosphorylation		Uniprot
T274	Phosphorylation		Uniprot
S285	Phosphorylation		Uniprot
S304	Phosphorylation		Uniprot
S314	Phosphorylation		Uniprot
S317	Phosphorylation		Uniprot
S318	Phosphorylation	O00141 (SGK1) , P31749 (AKT1)	Uniprot
T320	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
S341	Phosphorylation	Q15418 (RPS6KA1)	Uniprot
S344	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
S351	Phosphorylation		Uniprot
T356	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
K383	Ubiquitination		Uniprot
K477	Acetylation		Uniprot
S485	Phosphorylation	O14757 (CHEK1)	Uniprot
S486	Phosphorylation		Uniprot
T488	Phosphorylation		Uniprot
S551	Phosphorylation		Uniprot
K555	Ubiquitination		Uniprot
K560	Ubiquitination		Uniprot
S566	Phosphorylation		Uniprot
T568	Phosphorylation	O00141 (SGK1)	Uniprot
S569	Phosphorylation		Uniprot
S570	Phosphorylation		Uniprot
S576	Phosphorylation		Uniprot
S588	Phosphorylation	O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1)	Uniprot
S591	Phosphorylation		Uniprot
S595	Phosphorylation		Uniprot
S597	Phosphorylation		Uniprot
S600	Phosphorylation		Uniprot
S609	Phosphorylation		Uniprot
T613	Phosphorylation		Uniprot
S617	Phosphorylation		Uniprot
S621	Phosphorylation		Uniprot
S633	Phosphorylation		Uniprot
T642	Phosphorylation	O00141 (SGK1) , P31749 (AKT1)	Uniprot
S644	Phosphorylation		Uniprot
S648	Phosphorylation		Uniprot
S649	Phosphorylation		Uniprot
S666	Phosphorylation		Uniprot
S672	Phosphorylation		Uniprot
S673	Phosphorylation		Uniprot
S678	Phosphorylation		Uniprot
T701	Phosphorylation		Uniprot
S704	Phosphorylation		Uniprot
S707	Phosphorylation		Uniprot
T749	Phosphorylation		Uniprot
S750	Phosphorylation		Uniprot
S751	Phosphorylation	O00141 (SGK1) , Q15418 (RPS6KA1)	Uniprot
T752	Phosphorylation		Uniprot
S754	Phosphorylation		Uniprot
S757	Phosphorylation		Uniprot
S759	Phosphorylation		Uniprot
T763	Phosphorylation		Uniprot
S764	Phosphorylation		Uniprot
T766	Phosphorylation		Uniprot
S782	Phosphorylation		Uniprot
S787	Phosphorylation		Uniprot
S789	Phosphorylation		Uniprot
S806	Phosphorylation		Uniprot
S809	Phosphorylation		Uniprot
Y1191	Phosphorylation		Uniprot
S1207	Phosphorylation		Uniprot
S1246	Phosphorylation		Uniprot
K1255	Ubiquitination		Uniprot
K1267	Ubiquitination		Uniprot

Research Backgrounds

O60343_TBCD4_HUMAN

Function:

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

PTMs:

Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.

Subcellular Location:

Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.

Tissue Specificity:

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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AS160 Antibody - #AF2617