PITX2 Antibody - #DF13574

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Product: PITX2 Antibody
Catalog: DF13574
Description: Rabbit polyclonal antibody to PITX2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 35kDa; 35kD(Calculated).
Uniprot: Q99697
RRID: AB_2846593

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PITX2 Antibody detects endogenous levels of total PITX2.
RRID:
AB_2846593
Cite Format: Affinity Biosciences Cat# DF13574, RRID:AB_2846593.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q99697(PITX2_HUMAN) >>Visit HPA database.

Gene(ID):
PITX2(5308)
Sequence:
METNCRKLVSACVQLGVQPAAVECLFSKDSEIKKVEFTDSPESRKEAASSKFFPRQHPGANEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQNPASNLSACQYAVDRPV

PTMs - Q99697 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
T90 Phosphorylation P31749 (AKT1) , P31751 (AKT2)
S283 Phosphorylation

Research Backgrounds

Function:

Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform PTX2C is involved in left-right asymmetry the developing embryo (By similarity).

PTMs:

Phosphorylation at Thr-90 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the paired homeobox family. Bicoid subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

References

1). Weighted Correlation Network Analysis of Cancer Stem Cell-Related Prognostic Biomarkers in Esophageal Squamous Cell Carcinoma. Technology in Cancer Research & Treatment (PubMed: 35899307) [IF=2.8]

Application: IF/ICC    Species: Human    Sample: esophageal squamous cell carcinoma (ESCC)

Figure 3. Representative immunofluorescence of CILP (red), CST1 (red), DPP4 (red), F2RL2 (green), PITX2 (green), RIOX (red), ZFHX4 (green) in esophageal squamous cell carcinoma (ESCC) samples and normal tissues of 112 ESCC patients from Zhongshan Hospital, Fudan University.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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