Phospho-CaMKII alpha/delta (Thr286) Antibody - AF3493

	Price	Size
	$280	100ul
	$350	200ul

Product Information

Alternative Names:Expand▼

Alpha CaMKII; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha B subunit; Calcium/calmodulin dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMK II alpha subunit; CaMK-II subunit alpha; Camk2a; CAMKA; CaMKII; CaMKIINalpha; EC 2.7.11.17; KCC2A_HUMAN; KIAA0968; MGC123320; MGC139375; MGC155201; mKIAA0968; PK2CDD; PKCCD; R74975; zgc:112538; zgc:123320; Calcium / calmodulin dependent protein kinase 2 delta; Calcium / calmodulin dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase type II delta chain; Calcium/calmodulin-dependent protein kinase type II subunit delta; CAM kinase 2 delta; CAM kinase II delta; CaM kinase II delta subunit; CaM kinase II subunit delta; CaM-kinase II delta chain; CAMK 2d; CaMK-II delta subunit; CaMK-II subunit delta; CAMK2D; CAMKD; CAMKI; KCC2D_HUMAN; RATCAMKI;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-CaMK2 alpha/delta (Thr286) Antibody detects endogenous levels of CaMK2 alpha/delta only when phosphorylated at Threonine 286.

RRID:

AB_2834931
Please cite this product as: Affinity Biosciences Cat# AF3493, RRID:AB_2834931.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human CaMK2 alpha/delta around the phosphorylation site of Thr286.

Uniprot:

>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

CAMK2D,CAMK2A

Molecular Weight:

Observed Mol.Wt.: 50kD.
Predicted Mol.Wt.: 54kDa,56kDa(Calculated)..

Subcellular Location:

Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.

Tissue Specificity:

Q13557 KCC2D_HUMAN:
Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

Description:

CaMK2-delta a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

Sequence:

Q9UQM7 KCC2A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH

Q13557 KCC2D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIKPPCIPNGKENFSGGTSLWQNI

Function:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity.

Post-translational Modifications:

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.

Subcellular Location:

Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Dendrite.
Note: Postsynaptic lipid rafts.

Subunit Structure:

There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D. The corresponding proteins assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with BAALC. Interacts with MPDZ. Interacts with SYN1. Interacts with CAMK2N2. Interacts with SYNGAP1. Interacts with SYNPO2 (By similarity). Interacts with SHANK3. Interacts with GRIN2B. Interacts with CACNB2. Interacts with LRRC7. Interacts with GRM5.

Similarity:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Function:

Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.

Post-translational Modifications:

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

Subcellular Location:

Cell membrane>Sarcolemma>Peripheral membrane protein>Cytoplasmic side. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

Subunit Structure:

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with RRAD and CACNB2 (By similarity).

Similarity:

The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > Oocyte meiosis.(View pathway)
· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway.(View pathway)
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Glioma.(View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.(View pathway)
· Organismal Systems > Development > Axon guidance.(View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Sensory system > Olfactory transduction.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.(View pathway)
· Organismal Systems > Endocrine system > Insulin secretion.(View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.

ELISA analysis of AF3493 showing specificity to Phospho-CaMKII alpha/delta (Thr286) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Jiao L et al. lncRNA-ZFAS1 induces mitochondria-mediated apoptosis by causing cytosolic Ca2+ overload in myocardial infarction mice model. Cell Death Dis 2019 Dec 9;10(12):942 (PubMed: 31819041) [IF=6.304]

2). Ou J et al. Sinomenine Protects Against Morphine Dependence through the NMDAR1/CAMKII/CREB Pathway: A Possible Role of Astrocyte-Derived Exosomes. Molecules 2018 Sep 17;23(9) (PubMed: 30227624) [IF=3.267]

3). Zhou J et al. Dezocine attenuates the remifentanil-induced postoperative hyperalgesia by inhibition of phosphorylation of CaMKⅡα. Eur J Pharmacol 2020 Feb 15;869:172882 (PubMed: 31863769) [IF=3.263]

4). et al. Antiarrhythmic effects of ginsenoside Rg2 on calcium chloride–induced arrhythmias without oral toxicity.

5). et al. Cypermethrin induces Sertoli cell apoptosis through mitochondrial pathway associated with calcium.

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Catalog Number :

AF3493-BP
(Blocking peptide available as AF3493-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Horse

100%

Bovine

100%

Sheep

100%

Dog

100%

Chicken

100%

Rabbit

100%

Xenopus

88%

Zebrafish

88%

Pig

High similarity Medium similarity Low similarity No similarity

Q9UQM7/Q13557 as Substrate

Q13557
Q9UQM7

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S3	Phosphorylation		Uniprot
K43	Ubiquitination		Uniprot
K57	Ubiquitination		Uniprot
K69	Ubiquitination		Uniprot
S79	Phosphorylation		Uniprot
K138	Ubiquitination		Uniprot
S146	Phosphorylation		Uniprot
K227	Acetylation		Uniprot
K227	Ubiquitination		Uniprot
Y231	Phosphorylation		Uniprot
S235	Phosphorylation		Uniprot
K246	Ubiquitination		Uniprot
K251	Ubiquitination		Uniprot
T254	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
S276	Phosphorylation		Uniprot
S280	Phosphorylation		Uniprot
T287	Phosphorylation	Q13557 (CAMK2D)	Uniprot
K299	Methylation		Uniprot
K301	Methylation		Uniprot
T306	Phosphorylation		Uniprot
T307	Phosphorylation		Uniprot
T311	Phosphorylation		Uniprot
S315	Phosphorylation		Uniprot
K318	Acetylation		Uniprot
K318	Ubiquitination		Uniprot
S319	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
T331	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
S334	Phosphorylation		Uniprot
T336	Phosphorylation		Uniprot
T337	Phosphorylation		Uniprot
C373	S-Nitrosylation		Uniprot
S404	Phosphorylation		Uniprot
S441	Phosphorylation		Uniprot
S472	Phosphorylation		Uniprot
T474	Phosphorylation		Uniprot
S490	Phosphorylation		Uniprot

Site	PTM Type	Enzyme	Source
C6	S-Nitrosylation		Uniprot
Y13	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
K68	Ubiquitination		Uniprot
S78	Phosphorylation		Uniprot
K137	Ubiquitination		Uniprot
S145	Phosphorylation		Uniprot
K226	Acetylation		Uniprot
K226	Ubiquitination		Uniprot
Y230	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
K245	Ubiquitination		Uniprot
T253	Phosphorylation		Uniprot
K258	Methylation		Uniprot
C280	S-Nitrosylation		Uniprot
T286	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
K298	Methylation		Uniprot
K300	Methylation		Uniprot
T305	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
T306	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
T310	Phosphorylation		Uniprot
S314	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S318	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
T334	Phosphorylation		Uniprot
T336	Phosphorylation		Uniprot
T337	Phosphorylation		Uniprot

Q9UQM7/Q13557 as PTM Enzyme

Q13557
Q9UQM7

Substrate	Site	Source
O15084 (ANKRD28)	S1011	Uniprot
P08670 (VIM)	S83	Uniprot
P13688-8 (CEACAM1)	T457	Uniprot
P13688-8 (CEACAM1)	S459	Uniprot
P16220 (CREB1)	S133	Uniprot
P16220 (CREB1)	S142	Uniprot
P26678 (PLN)	T17	Uniprot
P55327 (TPD52)	S176	Uniprot
P56524 (HDAC4)	S210	Uniprot
P56524 (HDAC4)	S632	Uniprot
Q00613 (HSF1)	S230	Uniprot
Q03060-16 (CREM)	S87	Uniprot
Q08289-2 (CACNB2)	T499	Uniprot
Q08289 (CACNB2)	T554	Uniprot
Q13557 (CAMK2D)	T287	Uniprot
Q13557-1 (CAMK2D)	S315	Uniprot
Q13557-8 (CAMK2D)	S319	Uniprot
Q13557-1 (CAMK2D)	T331	Uniprot
Q13557-1 (CAMK2D)	S333	Uniprot
Q13557-1 (CAMK2D)	S334	Uniprot
Q13557-8 (CAMK2D)	T337	Uniprot
Q14524 (SCN5A)	S516	Uniprot
Q14524 (SCN5A)	T594	Uniprot
Q14571 (ITPR2)	S150	Uniprot
Q14654 (KCNJ11)	T224	Uniprot
Q16695 (HIST3H3)	S11	Uniprot
Q8WYL5 (SSH1)	S937	Uniprot
Q8WYL5 (SSH1)	S978	Uniprot
Q8WZ42 (TTN)	S4062	Uniprot
Q8WZ42 (TTN)	S11878	Uniprot
Q8WZ42 (TTN)	S12022	Uniprot
Q92736 (RYR2)	S2814	Uniprot
Q9UQL6 (HDAC5)	S498	Uniprot

Substrate	Site	Source
A8K161 (CAMK2A)	T286	Uniprot
A8K161 (CAMK2A)	T305	Uniprot
O00631 (SLN)	T5	Uniprot
O14490-2 (DLGAP1)	S44	Uniprot
O14713-2 (ITGB1BP1)	T38	Uniprot
O15273 (TCAP)	S157	Uniprot
O15273 (TCAP)	S161	Uniprot
O95999 (BCL10)	S138	Uniprot
P00533 (EGFR)	S768	Uniprot
P00533-1 (EGFR)	S1064	Uniprot
P00533 (EGFR)	S1070	Uniprot
P00533 (EGFR)	S1071	Uniprot
P00533-1 (EGFR)	S1081	Uniprot
P00533-1 (EGFR)	S1120	Uniprot
P00533 (EGFR)	S1166	Uniprot
P04626 (ERBB2)	T1172	Uniprot
P05556 (ITGB1)	T788	Uniprot
P05556 (ITGB1)	T789	Uniprot
P05783 (KRT18)	S53	Uniprot
P07101 (TH)	S19	Uniprot
P07101-4 (TH)	S35	Uniprot
P07101-3 (TH)	S40	Uniprot
P07101-4 (TH)	S44	Uniprot
P07101 (TH)	S71	Uniprot
P08670 (VIM)	S83	Uniprot
P09917 (ALOX5)	S272	Uniprot
P10636-6 (MAPT)	S204	Uniprot
P10636-8 (MAPT)	S262	Uniprot
P10636-2 (MAPT)	S267	Uniprot
P10636-6 (MAPT)	S298	Uniprot
P10636-8 (MAPT)	S356	Uniprot
P10636-8 (MAPT)	S416	Uniprot
P10636 (MAPT)	T548	Uniprot
P10636 (MAPT)	S552	Uniprot
P10636 (MAPT)	S579	Uniprot
P11831 (SRF)	S103	Uniprot
P11831 (SRF)	T160	Uniprot
P14136-1 (GFAP)	S13	Uniprot
P14136-1 (GFAP)	S17	Uniprot
P14136-1 (GFAP)	S38	Uniprot
P14136-1 (GFAP)	S393	Uniprot
P14921 (ETS1)	S251	Uniprot
P14921 (ETS1)	S257	Uniprot
P14921-1 (ETS1)	S282	Uniprot
P14921 (ETS1)	S285	Uniprot
P15036 (ETS2)	S246	Uniprot
P15036 (ETS2)	S310	Uniprot
P15036 (ETS2)	S313	Uniprot
P16070 (CD44)	S706	Uniprot
P16220 (CREB1)	S142	Uniprot
P16615-2 (ATP2A2)	S38	Uniprot
P16949 (STMN1)	S16	Uniprot
P17676-1 (CEBPB)	S325	Uniprot
P18433 (PTPRA)	S204	Uniprot
P20941-2 (PDC)	S2	Uniprot
P20941-2 (PDC)	S21	Uniprot
P20941-1 (PDC)	S54	Uniprot
P20941-1 (PDC)	S73	Uniprot
P21333 (FLNA)	S2523	Uniprot
P23677 (ITPKA)	T311	Uniprot
P26678 (PLN)	T17	Uniprot
P28329-3 (CHAT)	T456	Uniprot
P28329 (CHAT)	T574	Uniprot
P29475 (NOS1)	S852	Uniprot
P31645 (SLC6A4)	S13	Uniprot
P35222 (CTNNB1)	T332	Uniprot
P35222 (CTNNB1)	T472	Uniprot
P35222 (CTNNB1)	S552	Uniprot
P35270 (SPR)	S213	Uniprot
P35367 (HRH1)	T140	Uniprot
P35367 (HRH1)	T142	Uniprot
P35367 (HRH1)	S396	Uniprot
P35367 (HRH1)	S398	Uniprot
P35372 (OPRM1)	S268	Uniprot
P35372 (OPRM1)	S270	Uniprot
P35372 (OPRM1)	T372	Uniprot
P35462 (DRD3)	S229	Uniprot
P37840 (SNCA)	S129	Uniprot
P42261 (GRIA1)	S567	Uniprot
P42261 (GRIA1)	S832	Uniprot
P46527 (CDKN1B)	S10	Uniprot
P48058 (GRIA4)	S862	Uniprot
P51790 (CLCN3)	S109	Uniprot
P55042 (RRAD)	S273	Uniprot
P55055 (NR1H2)	S426	Uniprot
Q00613-1 (HSF1)	S230	Uniprot
Q00975 (CACNA1B)	S2120	Uniprot
Q01970 (PLCB3)	S537	Uniprot
Q04760 (GLO1)	T107	Uniprot
Q05397 (PTK2)	S843	Uniprot
Q05469 (LIPE)	S855	Uniprot
Q05682 (CALD1)	S643	Uniprot
Q05682 (CALD1)	S656	Uniprot
Q06210 (GFPT1)	S261	Uniprot
Q12879-1 (GRIN2A)	S1291	Uniprot
Q12959 (DLG1)	S232	Uniprot
Q13224 (GRIN2B)	S383	Uniprot
Q13224 (GRIN2B)	S1303	Uniprot
Q14653 (IRF3)	S386	Uniprot
Q14654 (KCNJ11)	T180	Uniprot
Q14654 (KCNJ11)	T224	Uniprot
Q14674 (ESPL1)	S1501	Uniprot
Q15121 (PEA15)	S116	Uniprot
Q15796 (SMAD2)	S110	Uniprot
Q15796-1 (SMAD2)	S240	Uniprot
Q15796-1 (SMAD2)	S260	Uniprot
Q4G163 (FBXO43)	T234	Uniprot
Q86UR5 (RIMS1)	S242	Uniprot
Q86UR5 (RIMS1)	S288	Uniprot
Q8IZP0 (ABI1)	S88	Uniprot
Q92736 (RYR2)	S2808	Uniprot
Q92736 (RYR2)	S2814	Uniprot
Q96A00 (PPP1R14A)	S130	Uniprot
Q96NW7 (LRRC7)	S1439	Uniprot
Q96PH1-4 (NOX5)	S475	Uniprot
Q96PM5 (RCHY1)	T154	Uniprot
Q96PM5 (RCHY1)	S155	Uniprot
Q96PV0-1 (SYNGAP1)	S1073	Uniprot
Q96PV0-1 (SYNGAP1)	T1077	Uniprot
Q96PV0-1 (SYNGAP1)	S1138	Uniprot
Q9BXL7 (CARD11)	S116	Uniprot
Q9NSC5 (HOMER3)	S120	Uniprot
Q9NSC5 (HOMER3)	S159	Uniprot
Q9NSC5 (HOMER3)	S176	Uniprot
Q9UBS5 (GABBR1)	S868	Uniprot
Q9UQL6 (HDAC5)	S259	Uniprot
Q9UQL6 (HDAC5)	S498	Uniprot
Q9UQM7-1 (CAMK2A)	T286	Uniprot
Q9UQM7-1 (CAMK2A)	T305	Uniprot
Q9UQM7 (CAMK2A)	T306	Uniprot
Q9UQM7 (CAMK2A)	S314	Uniprot
Q9Y496 (KIF3A)	S687	Uniprot
Q9Y4D2 (DAGLA)	S782	Uniprot
Q9Y4D2 (DAGLA)	S808	Uniprot

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Secondary antibody Goat Anti-Rabbit IgG (H+L) HRP (S0001) Goat Anti-Rabbit IgG (H+L) AP (S0003) Goat Anti-Rabbit IgG (H+L) Fluor594-conjugated (S0006) Goat Anti-Rabbit IgG (H+L) FITC-conjugated (S0008) Goat Anti-Rabbit IgG (H+L) CY3-conjugated (S0011) Goat Anti-Rabbit IgG (H+L) Fluor647-conjugated (S0013) Goat Anti-Rabbit IgG (H+L) TRITC-conjugated (S0015) Goat Anti-Rabbit IgG (H+L) Fluor488-conjugated (S0018)

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