CAP1 Antibody - #DF13310
(4)
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Protocols
Product Info
Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(100%)
Clonality:
Polyclonal
Specificity:
CAP1 Antibody detects endogenous levels of total CAP1.
RRID:
AB_2846329
Cite Format: Affinity Biosciences Cat# DF13310, RRID:AB_2846329.
Cite Format: Affinity Biosciences Cat# DF13310, RRID:AB_2846329.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Adenylyl cyclase associated protein 1; Adenylyl cyclase-associated protein 1; CAP 1; CAP; CAP, adenylate cyclase associated protein 1 (yeast); CAP1; CAP1 PEN; CAP1-PEN; CAP1_HUMAN;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q01518 CAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADMQNLVERLERAVGRLEAVSHTSDMHRGYADSPSKAGAAPYVQAFDSLLAGPVAEYLKISKEIGGDVQKHAEMVHTGLKLERALLVTASQCQQPAENKLSDLLAPISEQIKEVITFREKNRGSKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSCPPPPPPCPPPPPVSTISCSYESASRSSLFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Horse
67
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q01518 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K81 | Acetylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| C93 | S-Nitrosylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| Y164 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K184 | Methylation | Uniprot | |
| K209 | Acetylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| K279 | Methylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K287 | Methylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K298 | Methylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| K312 | Acetylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| K348 | Sumoylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| Y354 | Phosphorylation | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| T407 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K412 | Acetylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| Y419 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot |
Research Backgrounds
Function:
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Subcellular Location:
Cell membrane>Peripheral membrane protein.
Subunit Structure:
Homodimer. Binds actin monomers.
Family&Domains:
Belongs to the CAP family.
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For Research Use Only.
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