Pericentrin - Centrosome Marker Antibody - #DF13210

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Product: Pericentrin - Centrosome Marker Antibody
Catalog: DF13210
Description: Rabbit polyclonal antibody to Pericentrin - Centrosome Marker
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 378kDa; 378kD(Calculated).
Uniprot: O95613
RRID: AB_2846229

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
Pericentrin - Centrosome Marker Antibody detects endogenous levels of total Pericentrin - Centrosome Marker.
RRID:
AB_2846229
Cite Format: Affinity Biosciences Cat# DF13210, RRID:AB_2846229.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Centrosome Marker; Ken; Kendrin; KIAA0402; MOPD2; PCN; PCNT 2; PCNT; PCNT B; PCNT_HUMAN; PCNT1; PCNT2; PCNTB; PCTN2; Pericentrin 1; Pericentrin 2; Pericentrin 380; Pericentrin; Pericentrin B; Pericentrin-B; SCKL4;

Immunogens

Immunogen:
Uniprot:

O95613(PCNT_HUMAN) >>Visit HPA database.

Gene(ID):
PCNT(5116)
Expression:
O95613 PCNT_HUMAN:

Expressed in all tissues tested, including placenta, liver, kidney and thymus.

Sequence:
MEVEQEQRRRKVEAGRTKLAHFRQRKTKGDSSHSEKKTAKRKGSAVDASVQEESPVTKEDSALCGGGDICKSTSCDDTPDGAGGAFAAQPEDCDGEKREDLEQLQQKQVNDHPPEQCGMFTVSDHPPEQHGMFTVGDHPPEQRGMFTVSDHPPEQHGMFTVSDHPPEQRGMFTISDHQPEQRGMFTVSDHTPEQRGIFTISDHPAEQRGMFTKECEQECELAITDLESGREDEAGLHQSQAVHGLELEALRLSLSNMHTAQLELTQANLQKEKETALTELREMLNSRRAQELALLQSRQQHELELLREQHAREKEEVVLRCGQEAAELKEKLQSEMEKNAQIVKTLKEDWESEKDLCLENLRKELSAKHQSEMEDLQNQFQKELAEQRAELEKIFQDKNQAERALRNLESHHQAAIEKLREDLQSEHGRCLEDLEFKFKESEKEKQLELENLQASYEDLKAQSQEEIRRLWSQLDSARTSRQELSELHEQLLARTSRVEDLEQLKQREKTQHESELEQLRIYFEKKLRDAEKTYQEDLTLLQQRLQGAREDALLDSVEVGLSCVGLEEKPEKGRKDHVDELEPERHKESLPRFQAELEESHRHQLEALESPLCIQHEGHVSDRCCVETSALGHEWRLEPSEGHSQELPWVHLQGVQDGDLEADTERAARVLGLETEHKVQLSLLQTELKEEIELLKIENRNLYGKLQHETRLKDDLEKVKHNLIEDHQKELNNAKQKTELMKQEFQRKETDWKVMKEELQREAEEKLTLMLLELREKAESEKQTIINKFELREAEMRQLQDQQAAQILDLERSLTEQQGRLQQLEQDLTSDDALHCSQCGREPPTAQDGELAALHVKEDCALQLMLARSRFLEERKEITEKFSAEQDAFLQEAQEQHARELQLLQERHQQQLLSVTAELEARHQAALGELTASLESKQGALLAARVAELQTKHAADLGALETRHLSSLDSLESCYLSEFQTIREEHRQALELLRADFEEQLWKKDSLHQTILTQELEKLKRKHEGELQSVRDHLRTEVSTELAGTVAHELQGVHQGEFGSEKKTALHEKEETLRLQSAQAQPFHQEEKESLSLQLQKKNHQVQQLKDQVLSLSHEIEECRSELEVLQQRRERENREGANLLSMLKADVNLSHSERGALQDALRRLLGLFGETLRAAVTLRSRIGERVGLCLDDAGAGLALSTAPALEETWSDVALPELDRTLSECAEMSSVAEISSHMRESFLMSPESVRECEQPIRRVFQSLSLAVDGLMEMALDSSRQLEEARQIHSRFEKEFSFKNEETAQVVRKHQELLECLKEESAAKAELALELHKTQGTLEGFKVETADLKEVLAGKEDSEHRLVLELESLRRQLQQAAQEQAALREECTRLWSRGEATATDAEAREAALRKEVEDLTKEQSETRKQAEKDRSALLSQMKILESELEEQLSQHRGCAKQAEAVTALEQQVASLDKHLRNQRQFMDEQAAEREHEREEFQQEIQRLEGQLRQAAKPQPWGPRDSQQAPLDGEVELLQQKLREKLDEFNELAIQKESADRQVLMQEEEIKRLEEMNINIRKKVAQLQEEVEKQKNIVKGLEQDKEVLKKQQMSSLLLASTLQSTLDAGRCPEPPSGSPPEGPEIQLEVTQRALLRRESEVLDLKEQLEKMKGDLESKNEEILHLNLKLDMQNSQTAVSLRELEEENTSLKVIYTRSSEIEELKATIENLQENQKRLQKEKAEEIEQLHEVIEKLQHELSLMGPVVHEVSDSQAGSLQSELLCSQAGGPRGQALQGELEAALEAKEALSRLLADQERRHSQALEALQQRLQGAEEAAELQLAELERNVALREAEVEDMASRIQEFEAALKAKEATIAERNLEIDALNQRKAAHSAELEAVLLALARIRRALEQQPLAAGAAPPELQWLRAQCARLSRQLQVLHQRFLRCQVELDRRQARRATAHTRVPGAHPQPRMDGGAKAQVTGDVEASHDAALEPVVPDPQGDLQPVLVTLKDAPLCKQEGVMSVLTVCQRQLQSELLLVKNEMRLSLEDGGKGKEKVLEDCQLPKVDLVAQVKQLQEKLNRLLYSMTFQNVDAADTKSLWPMASAHLLESSWSDDSCDGEEPDISPHIDTCDANTATGGVTDVIKNQAIDACDANTTPGGVTDVIKNWDSLIPDEMPDSPIQEKSECQDMSLSSPTSVLGGSRHQSHTAEAGPRKSPVGMLDLSSWSSPEVLRKDWTLEPWPSLPVTPHSGALSLCSADTSLGDRADTSLPQTQGPGLLCSPGVSAAALALQWAESPPADDHHVQRTAVEKDVEDFITTSFDSQETLSSPPPGLEGKADRSEKSDGSGFGARLSPGSGGPEAQTAGPVTPASISGRFQPLPEAMKEKEVRPKHVKALLQMVRDESHQILALSEGLAPPSGEPHPPRKEDEIQDISLHGGKTQEVPTACPDWRGDLLQVVQEAFEKEQEMQGVELQPRLSGSDLGGHSSLLERLEKIIREQGDLQEKSLEHLRLPDRSSLLSEIQALRAQLRMTHLQNQEKLQHLRTALTSAEARGSQQEHQLRRQVELLAYKVEQEKCIAGDLQKTLSEEQEKANSVQKLLAAEQTVVRDLKSDLCESRQKSEQLSRSLCEVQQEVLQLRSMLSSKENELKAALQELESEQGKGRALQSQLEEEQLRHLQRESQSAKALEELRASLETQRAQSSRLCVALKHEQTAKDNLQKELRIEHSRCEALLAQERSQLSELQKDLAAEKSRTLELSEALRHERLLTEQLSQRTQEACVHQDTQAHHALLQKLKEEKSRVVDLQAMLEKVQQQALHSQQQLEAEAQKHCEALRREKEVSATLKSTVEALHTQKRELRCSLEREREKPAWLQAELEQSHPRLKEQEGRKAARRSAEARQSPAAAEQWRKWQRDKEKLRELELQRQRDLHKIKQLQQTVRDLESKDEVPGSRLHLGSARRAAGSDADHLREQQRELEAMRQRLLSAARLLTSFTSQAVDRTVNDWTSSNEKAVMSLLHTLEELKSDLSRPTSSQKKMAAELQFQFVDVLLKDNVSLTKALSTVTQEKLELSRAVSKLEKLLKHHLQKGCSPSRSERSAWKPDETAPQSSLRRPDPGRLPPAASEEAHTSNVKMEKLYLHYLRAESFRKALIYQKKYLLLLIGGFQDSEQETLSMIAHLGVFPSKAERKITSRPFTRFRTAVRVVIAILRLRFLVKKWQEVDRKGALAQGKAPRPGPRARQPQSPPRTRESPPTRDVPSGHTRDPARGRRLAAAASPHSGGRATPSPNSRLERSLTASQDPEHSLTEYIHHLEVIQQRLGGVLPDSTSKKSCHPMIKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Horse
88
Dog
88
Chicken
71
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95613 As Substrate

Site PTM Type Enzyme
S44 Phosphorylation
S54 Phosphorylation
S61 Phosphorylation
S175 Phosphorylation
S188 Phosphorylation
T191 Phosphorylation
S201 Phosphorylation
S253 Phosphorylation
S255 Phosphorylation
T259 Phosphorylation
K271 Ubiquitination
K273 Ubiquitination
K338 Acetylation
K354 Ubiquitination
K418 Ubiquitination
K437 Ubiquitination
S455 Phosphorylation
T479 Phosphorylation
K505 Ubiquitination
S514 Phosphorylation
K532 Ubiquitination
S556 Phosphorylation
S562 Phosphorylation
K569 Ubiquitination
S589 Phosphorylation
S610 Phosphorylation
S644 Phosphorylation
S682 Phosphorylation
K696 Ubiquitination
K788 Ubiquitination
S813 Phosphorylation
T815 Phosphorylation
S837 Phosphorylation
K881 Ubiquitination
K952 Ubiquitination
T962 Phosphorylation
K1004 Ubiquitination
S1006 Phosphorylation
K1018 Ubiquitination
K1022 Ubiquitination
K1069 Ubiquitination
K1088 Ubiquitination
S1142 Phosphorylation
K1145 Ubiquitination
T1178 Phosphorylation
T1209 Phosphorylation P53350 (PLK1)
T1221 Phosphorylation P53350 (PLK1)
S1235 Phosphorylation P53350 (PLK1)
S1241 Phosphorylation P53350 (PLK1)
S1245 Phosphorylation
S1289 Phosphorylation
K1293 Ubiquitination
S1296 Phosphorylation
K1298 Ubiquitination
K1308 Ubiquitination
K1317 Ubiquitination
K1341 Ubiquitination
K1348 Ubiquitination
S1367 Phosphorylation
S1430 Phosphorylation
K1437 Ubiquitination
K1472 Ubiquitination
K1535 Ubiquitination
K1550 Ubiquitination
S1632 Phosphorylation
S1653 Phosphorylation
S1671 Phosphorylation
S1688 Phosphorylation
S1693 Phosphorylation
S1703 Phosphorylation
K1705 Ubiquitination
R1710 Methylation
K1718 Ubiquitination
S1770 Phosphorylation
K1799 Ubiquitination
S1814 Phosphorylation
K1864 Ubiquitination
K1884 Ubiquitination
K2015 Ubiquitination
K2038 Ubiquitination
S2044 Phosphorylation
K2050 Ubiquitination
K2054 Ubiquitination
K2071 Ubiquitination
K2076 Ubiquitination
S2114 Phosphorylation
T2155 Phosphorylation
S2168 Phosphorylation
S2177 Phosphorylation
S2192 Phosphorylation
S2204 Phosphorylation
K2213 Acetylation
S2214 Phosphorylation
S2259 Phosphorylation
S2279 Phosphorylation
S2327 Phosphorylation
S2339 Phosphorylation
S2342 Phosphorylation
S2345 Phosphorylation
S2352 Phosphorylation
S2355 Phosphorylation
T2367 Phosphorylation
S2370 Phosphorylation
S2433 Phosphorylation
K2438 Ubiquitination
K2463 Ubiquitination
S2477 Phosphorylation
S2479 Phosphorylation
S2486 Phosphorylation
K2504 Ubiquitination
S2505 Phosphorylation
S2519 Phosphorylation
K2538 Ubiquitination
K2570 Ubiquitination
K2575 Ubiquitination
K2583 Ubiquitination
T2584 Phosphorylation
S2586 Phosphorylation
S2594 Phosphorylation
K2597 Ubiquitination
K2610 Ubiquitination
K2649 Ubiquitination
K2661 Ubiquitination
K2685 Ubiquitination
K2709 Acetylation
K2709 Ubiquitination
K2720 Ubiquitination
K2745 Ubiquitination
K2810 Ubiquitination
K2828 Ubiquitination
K2854 Ubiquitination
S2860 Phosphorylation
K2867 Ubiquitination
S2878 Phosphorylation
S2894 Phosphorylation
S2900 Phosphorylation
K2932 Ubiquitination
K2944 Ubiquitination
S2956 Phosphorylation
S2963 Phosphorylation
R2999 Methylation
K3010 Ubiquitination
S3014 Phosphorylation
K3023 Ubiquitination
K3034 Ubiquitination
K3035 Ubiquitination
K3057 Ubiquitination
K3066 Ubiquitination
S3070 Phosphorylation
S3074 Phosphorylation
K3075 Acetylation
K3075 Ubiquitination
K3081 Ubiquitination
K3086 Ubiquitination
K3099 Ubiquitination
S3108 Phosphorylation
K3131 Ubiquitination
Y3139 Phosphorylation
S3166 Phosphorylation
T3189 Phosphorylation
S3190 Phosphorylation
K3229 Ubiquitination
S3242 Phosphorylation
S3249 Phosphorylation
S3274 Phosphorylation
S3277 Phosphorylation
T3282 Phosphorylation
S3284 Phosphorylation
S3287 Phosphorylation
S3292 Phosphorylation
S3302 Phosphorylation
S3324 Phosphorylation
S3326 Phosphorylation
K3327 Ubiquitination
K3328 Ubiquitination

Research Backgrounds

Function:

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.

PTMs:

Cleaved during mitotis which leads to removal of CDK5RAP2 from the centrosome and promotes centriole disengagement and subsequent centriole separation. The C-terminal fragment is rapidly degraded following cleavage.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested, including placenta, liver, kidney and thymus.

Subunit Structure:

Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity (By similarity). Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2. Interacts with isoform 1 of NEK2. Interacts with CEP131. Interacts with CCDC13. Interacts with CEP68. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.

Family&Domains:

Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

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