Actin Plant Antibody
Price Size
$280 100ul
$350 200ul

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  • Product Name
    Actin Plant Antibody
  • Catalog No.
    T0015
  • RRID
    AB_2846217
  • Source
    Rabbit
  • Application
    WB,ELISA(peptide)
  • Reactivity
    Plants
  • UniProt
  • Mol.Wt
    45kD;
  • Concentration
    1mg/ml
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Product Information

Applications:

WB: 1:500-1:3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Plants

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

ACTIN Plant Antibody detects endogenous levels of plant ACTIN.

RRID:

AB_2846217
Please cite this product as: Affinity Biosciences Cat# T0015, RRID:AB_2846217.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from plant actin.

Uniprot:



>>Visit The Human Protein Atlas

Molecular Weight:

Observed Mol.Wt.: 45kD.
Predicted Mol.Wt.: Not available.

Not available

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Catalog Number :

T0015-BP
(Blocking peptide available as T0015-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
0%
Horse
0%
Bovine
0%
Sheep
0%
Dog
0%
Xenopus
0%
Zebrafish
0%
Chicken
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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