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  • Product Name
    Phospho-Trk B (Tyr706) Antibody
  • Catalog No.
    AF3461
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
  • UniProt
  • Mol.Wt.
    145kDa,92kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

AI848316; BDNF tropomyosine receptor kinase B; BDNF/NT 3 growth factors receptor; BDNF/NT-3 growth factors receptor; Brain derived neurotrophic factor receptor; C030027L06Rik; EC 2.7.10.1; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP95 TrkB; Neurotrophic receptor tyrosine kinase 2; Neurotrophic tyrosine kinase receptor type 2; Neurotrophin receptor tyrosine kinase type 2; NTRK 2; Ntrk2; NTRK2_HUMAN; Obesity, hyperphagia, and developmental delay, included; RATTRKB1; Tkrb; Trk B; Trk-B; TRKB; TrkB tyrosine kinase; TRKB1; Tropomyosin related kinase B; tyrosine kinase receptor B; Tyrosine receptor kinase B;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-Trk B (Tyr706) Antibody detects endogenous levels of Trk B only when phosphorylated at Tyrosine 706.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Trk B around the phosphorylation site of Tyr706.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

Q16620

Molecular Weight:

Observed Mol.Wt.: 145kDa.
Predicted Mol.Wt.: 92kDa.

Subcellular Location:

Membrane.

Tissue Specificity:

Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.

Description:

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation.

Sequence:
        10         20         30         40         50
MSSWIRWHGP AMARLWGFCW LVVGFWRAAF ACPTSCKCSA SRIWCSDPSP
60 70 80 90 100
GIVAFPRLEP NSVDPENITE IFIANQKRLE IINEDDVEAY VGLRNLTIVD
110 120 130 140 150
SGLKFVAHKA FLKNSNLQHI NFTRNKLTSL SRKHFRHLDL SELILVGNPF
160 170 180 190 200
TCSCDIMWIK TLQEAKSSPD TQDLYCLNES SKNIPLANLQ IPNCGLPSAN
210 220 230 240 250
LAAPNLTVEE GKSITLSCSV AGDPVPNMYW DVGNLVSKHM NETSHTQGSL
260 270 280 290 300
RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH
310 320 330 340 350
WCIPFTVKGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN
360 370 380 390 400
PTHMNNGDYT LIAKNEYGKD EKQISAHFMG WPGIDDGANP NYPDVIYEDY
410 420 430 440 450
GTAANDIGDT TNRSNEIPST DVTDKTGREH LSVYAVVVIA SVVGFCLLVM
460 470 480 490 500
LFLLKLARHS KFGMKGPASV ISNDDDSASP LHHISNGSNT PSSSEGGPDA
510 520 530 540 550
VIIGMTKIPV IENPQYFGIT NSQLKPDTFV QHIKRHNIVL KRELGEGAFG
560 570 580 590 600
KVFLAECYNL CPEQDKILVA VKTLKDASDN ARKDFHREAE LLTNLQHEHI
610 620 630 640 650
VKFYGVCVEG DPLIMVFEYM KHGDLNKFLR AHGPDAVLMA EGNPPTELTQ
660 670 680 690 700
SQMLHIAQQI AAGMVYLASQ HFVHRDLATR NCLVGENLLV KIGDFGMSRD
710 720 730 740 750
VYSTDYYRVG GHTMLPIRWM PPESIMYRKF TTESDVWSLG VVLWEIFTYG
760 770 780 790 800
KQPWYQLSNN EVIECITQGR VLQRPRTCPQ EVYELMLGCW QREPHMRKNI
810 820
KGIHTLLQNL AKASPVYLDI LG

Background

Function:

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

Post-translational Modifications:

Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated.Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor (By similarity).

Subcellular Location:

Endosome;Plasma membrane;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Interacts (phosphorylated upon activation by BDNF) with SHC1; mediates SHC1 phosphorylation and activation. Interacts (phosphorylated upon activation by BDNF) with PLCG1 and/or PLCG2; mediates PLCG1 phosphorylation and activation. Interacts with SH2B1 and SH2B2. Interacts with NGFR; may regulate the ligand specificity of the receptor. Interacts (phosphorylated upon ligand-binding) with SH2D1A; regulates NTRK2. Interacts with SQSTM1 and KIDINS220 (By similarity). Interacts (phosphorylated upon ligand-binding) with FRS2; activates the MAPK signaling pathway. Interacts with APPL1 (By similarity).

Similarity:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)

WB Images(1)


IHC Images(1)


IF/ICC Images(1)


ELISA analysis of AF3461 showing specificity to Phospho-Trk B (Tyr706) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Zhao Y et al. TAT-Ngn2 Enhances Cognitive Function Recovery and Regulates Caspase-Dependent and Mitochondrial Apoptotic Pathways After Experimental Stroke. Front Cell Neurosci 2018 Dec 14;12:475 (PubMed: 30618628) [IF=3.900]

2). Zhao ZH et al. PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury. Cell Mol Neurobiol 2017 May;37(4):707-715 (PubMed: 27443384) [IF=3.811]

Application: WB    Species:mouse;    Sample:Not available


3). Zhan G et al. PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat. Psychopharmacology (Berl) 2018 Sep 26 (PubMed: 30259075) [IF=3.424]

4). Huang N et al. Alterations in the BDNF-mTOR Signaling Pathway in the Spinal Cord Contribute to Hyperalgesia in a Rodent Model of Chronic Restraint Stress. Neuroscience 2019 Mar 30 (PubMed: 30940563) [IF=3.244]

5). Fang X et al. Brain-derived neurotrophic factor-TrkB signaling in the medial prefrontal cortex plays a role in the anhedonia-like phenotype after spared nerve injury. Eur Arch Psychiatry Clin Neurosci 2020 Mar;270(2):195-205 (PubMed: 29882089) [IF=3.192]

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Catalog Number :

AF3461-BP
(Blocking peptide available as AF3461-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Chicken
100%
Sheep
100%
Rabbit
100%
Horse
100%
Zebrafish
0%
Dog
0%
Bovine
0%
Pig
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
Q16620 as Substrate
Site PTM Type Enzyme
N67 N-Glycosylation
N95 N-Glycosylation
N121 N-Glycosylation
S141 Phosphorylation
T151 Phosphorylation
S153 Phosphorylation
T161 Phosphorylation
N178 N-Glycosylation
N205 N-Glycosylation
N241 N-Glycosylation
T253 Phosphorylation
N254 N-Glycosylation
S256 Phosphorylation
S257 Phosphorylation
S260 Phosphorylation
N280 N-Glycosylation
N338 N-Glycosylation
N412 N-Glycosylation
K461 Acetylation
S479 Phosphorylation
Y490 Phosphorylation
T506 Phosphorylation
Y516 Phosphorylation Q16620 (NTRK2)
Y558 Phosphorylation
T573 Phosphorylation
Y670 Phosphorylation
Y674 Phosphorylation
Y675 Phosphorylation
S698 Phosphorylation
Y702 Phosphorylation
Y706 Phosphorylation
Y707 Phosphorylation
Y785 Phosphorylation
Y817 Phosphorylation
Q16620 as PTM Enzyme
Substrate Site Source
O43559 (FRS3) Y417 Uniprot
O43559 (FRS3) Y455 Uniprot
P22001 (KCNA3) Y161 Uniprot
P22001 (KCNA3) Y162 Uniprot
P22001 (KCNA3) Y163 Uniprot
P22001 (KCNA3) Y499 Uniprot
Q00535 (CDK5) Y15 Uniprot
Q13009 (TIAM1) Y829 Uniprot
Q16620 (NTRK2) Y516 Uniprot
Q16620-4 (NTRK2) Y532 Uniprot
Q16620-1 (NTRK2) Y702 Uniprot
Q16620-1 (NTRK2) Y706 Uniprot
Q16620-1 (NTRK2) Y707 Uniprot
Q16620-4 (NTRK2) Y718 Uniprot
Q16620-4 (NTRK2) Y722 Uniprot
Q16620-4 (NTRK2) Y723 Uniprot
Q16620-1 (NTRK2) Y817 Uniprot
Q16620-4 (NTRK2) Y833 Uniprot
Q92529-2 (SHC3) Y218 Uniprot
Q92529-2 (SHC3) Y219 Uniprot
Q92529-2 (SHC3) Y256 Uniprot
Q92529-2 (SHC3) Y257 Uniprot
Q92529-2 (SHC3) Y283 Uniprot
Q92529-2 (SHC3) Y301 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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