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  • Product Name
    Phospho-AMPK alpha (Thr172) Antibody
  • Catalog No.
    AF3423
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity:
    Human, Mouse, Rat
  • Prediction:
    Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
  • UniProt
  • Mol.Wt.
    62kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AAPK1_HUMAN; ACACA kinase; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1; AMPK alpha 1 chain; AMPK; AMPK subunit alpha-1; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; HMGCR kinase; hormone sensitive lipase kinase; Hydroxymethylglutaryl CoA reductase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; OTTHUMP00000161795; OTTHUMP00000161796; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; SNF1A; Tau protein kinase PRKAA1; wu:fa94c10; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AAPK2_HUMAN; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPKalpha2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; PRKAA; PRKAA2; Protein kinase AMP activated alpha 2 catalytic subunit; Protein kinase AMP activated catalytic subunit alpha 2;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF 1:100-500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-AMPK alpha (Thr172) Antibody detects endogenous levels of AMPK alpha only when phosphorylated at Threonine 172.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 62kDa.
Predicted Mol.Wt.: 65kDa.

Description:

AMPKA2 a protein kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma).

Sequence:
        10         20         30         40         50
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG
60 70 80 90 100
HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI
110 120 130 140 150
FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD
160 170 180 190 200
LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL
210 220 230 240 250
YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP
260 270 280 290 300
SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID
310 320 330 340 350
DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD
360 370 380 390 400
FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ
410 420 430 440 450
GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP
460 470 480 490 500
VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY
510 520 530 540 550
RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC

ANLIKILAQ

Background

Function:

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochontrial import (By similarity). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Post-translational Modifications:

Ubiquitinated.Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B.

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

Similarity:

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal.(View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Insulin signaling pathway.(View pathway)
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
· Organismal Systems > Environmental adaptation > Circadian rhythm.(View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Aging > Longevity regulating pathway.(View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species.(View pathway)

Western blot analysis of extracts from PANC1, using Phospho-AMPK alpha (Thr172) Antibody. The lane on the left was treated with blocking peptide.
Phospho-AMPK alpha (Thr172) Antibody for IHC in human colon.
AF3423 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
af3423 staining C2C12 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ELISA analysis of AF3423 showing specificity to Phospho-AMPK alpha (Thr172) peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Zhang F et al. Anti-osteoporosis activity of Sanguinarine in preosteoblast MC3T3-E1 cells and an ovariectomized rat model. J Cell Physiol 2018 Jun;233(6):4626-4633 (PubMed: 28926099) [IF=4.522]

Application: WB    Species:mouse;    Sample:Not available

Figure 3. Compound C reversed the effects of Sanguinarine on the differentiation of MC3T3-E1 cells. MC3T3-E1 cells were pretreated with 10 μM Compound C for 1 h and then exposed to 2 M Sanguinarine (SAN). Mock cells were cultured without any treatment. (A, B) At 48 h after treatment, the levels of AMPK1 and p-AMPK (A), and ALP activity in the cultured medium (B) were analyzed. (C) mRNA levels of Bmp2, Osx and Opg were detected by real-time PCR. (D) Protein levels of Bmp2, Smad1, pSmad1, Osx and Opg were determined by Western blot. ***P <0.001 versus DMSO; +++P <0.001 versus Compound C.


2). Xiao L et al. Hydrogen sulfide improves endothelial dysfunction in hypertension by activating peroxisome proliferator-activated receptor delta/endothelial nitric oxide synthase signaling. J Hypertens 2018 Mar;36(3):651-665 (PubMed: 29084084) [IF=4.209]

3). Zhao J et al. Inhibition of CCL19 benefits non‑alcoholic fatty liver disease by inhibiting TLR4/NF‑κB‑p65 signaling. Mol Med Rep 2018 Sep 14 (PubMed: 30221732)

4). Li S et al. Effect of CAPE-pNO2 against type 2 diabetes mellitus via the AMPK/GLUT4/ GSK3β/PPARα pathway in HFD/STZ-induced diabetic mice. Eur J Pharmacol 2019 Mar 15;853:1-10 (PubMed: 30885574)

5). Huang X et al. High expressions of LDHA and AMPK as prognostic biomarkers for breast cancer. Breast 2016 Dec;30:39-46 (PubMed: 27598996)

Application: WB    Species:human;    Sample:Not available

Fig. 1. LDHA and AMPK were up-regulated synchronously in breast cancer. A. Expression levels of LDHA, AMPK and pAMPK were assessed by Western blot (above) and gray image scanning (below) in eight different breast cancer cell lines, including four NTNBC cell lines and four TNBC cell lines. GAPDH was used as a loading control. B. Expression levels of LDHA and AMPK were determined by qRT-PCR (above). The differences between TNBC and NTNBC cell lines were analyzed (below). GAPDH was used as an internal control. C. Expression levels of LDHA, AMPK and pAMPK were assessed by Western blot (above) and gray image scanning (below) in eight different breast cancer tissues, including four NTNBC tissues and four TNBC tissues. GAPDH was used as a loading control. D. Expression levels of LDHA and AMPK were determined by qRT-PCR (above). The diffe


6). Li M et al. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. J Virol 2018 Mar 28;92(8) (PubMed: 29386287)

7). Lin H et al. Rapamycin Supplementation May Ameliorate Erectile Function in Rats With Streptozotocin-Induced Type 1 Diabetes by Inducing Autophagy and Inhibiting Apoptosis, Endothelial Dysfunction, and Corporal Fibrosis. J Sex Med 2018 Sep;15(9):1246-1259 (PubMed: 30224017)

8). Cheng Y et al. Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. Int J Mol Med 2019 May 30 (PubMed: 31173178)

9). Li M et al. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. J Virol 2018 Mar 28;92(8) (PubMed: 29386287)

10). Dong W et al. Zoledronate and high glucose levels influence osteoclast differentiation and bone absorption via the AMPK pathway. Biochem Biophys Res Commun 2018 Oct 12 (PubMed: 30322621)

11). et al. Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells.

12). Li X et al. Enhancement of Glucose Metabolism via PGC-1α Participates in the Cardioprotection of Chronic Intermittent Hypobaric Hypoxia. Front Physiol 2016 Jun 8;7:219 (PubMed: 27375497)

Application: WB    Species:rat;    Sample:Not available

FIGURE 4 | Effect of CIHH on the protein expression of AMPK, p-AMPK, PDK4 and PGC-1α in left ventricular myocytes before and after I/R. (A) Protein expression of AMPK and p-AMPK; (B) Protein expression of PDK4; (C) Protein expression of PGC-1α. CON, control group; CIHH, chronic intermittent hypobaric hypoxia; Pre, pre-ischemia; I/R, ischemia/reperfusion. Data are expressed as the mean ± SD (n = 6 in each group). *P < 0.05 vs. corresponding CON group.


13). Ma MQ et al. A 6 hour therapeutic window, optimal for interventions targeting AMPK synergism and apoptosis antagonism, for cardioprotection against myocardial ischemic injury: an experimental study on rats. Am J Cardiovasc Dis 2015 Mar 20;5(1):63-71 (PubMed: 26064793)

14). et al. CB2 receptor agonist JWH133 activates AMPK to inhibit growth of C6 glioma cells.

15). Deng Z et al. Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis. Biochem Biophys Res Commun 2017 Dec 9;494(1-2):278-284 (PubMed: 29024627)

Application: WB    Species:mouse;    Sample:Not available

Fig. 4. Myostatin regulated translation elongation through AMP. C2C12 myotubes were treated with various concentration recombinant myostatin (0, 0.01,0.1, 1, 2, 3 µg/ml) for 48 h andthen lysed and cellular extracts were analyzed by Western blot with anti-AMPK(A).


16). et al. Hydrogen sulfide improves endothelial dysfunction in hypertension by activating peroxisome proliferatoractivated receptor delta/endothelial nitric oxide synthase signaling.

17). Zhang DS et al. Role of Phosphorylated AMP-Activated Protein Kinase (AMPK) in Myocardial Insulin Resistance After Myocardial Ischemia-Reperfusion During Cardiopulmonary Bypass Surgery in Dogs. Med Sci Monit 2019 Jun 4;25:4149-4158 (PubMed: 31160548)

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Catalog Number :

AF3423-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Zebrafish
100%
Chicken
100%
Rabbit
100%
Xenopus
100%
Dog
100%
Bovine
100%
Sheep
100%
Pig
100%
Horse
0%
High similarity Medium similarity Low similarity No similarity
Q13131/P54646 as Substrate
Site PTM Type Enzyme
S6 Phosphorylation
T32 Phosphorylation
K40 Ubiquitination
K45 Ubiquitination
K52 Ubiquitination
K56 Ubiquitination
K71 Ubiquitination
K80 Acetylation
K80 Ubiquitination
K152 Ubiquitination
S172 Phosphorylation
S176 Phosphorylation
T183 Phosphorylation Q8TDC3-2 (BRSK1) , Q16584 (MAP3K11) , Q15831 (STK11) , Q13554 (CAMK2B) , Q8N5S9 (CAMKK1) , Q13131 (PRKAA1) , Q96RR4 (CAMKK2) , Q8IWQ3 (BRSK2)
S184 Phosphorylation
C185 S-Nitrosylation
S187 Phosphorylation
Y190 Phosphorylation
Y247 Phosphorylation
K266 Ubiquitination
K271 Ubiquitination
K280 Ubiquitination
K285 Ubiquitination
S293 Phosphorylation
Y294 Phosphorylation
T355 Phosphorylation
S356 Phosphorylation
S360 Phosphorylation Q13131 (PRKAA1) , O75385 (ULK1)
T368 Phosphorylation O75385 (ULK1)
T382 Phosphorylation
T388 Phosphorylation Q13131 (PRKAA1)
K396 Ubiquitination
S397 Phosphorylation O75385 (ULK1)
K406 Sumoylation
K406 Ubiquitination
K429 Ubiquitination
Y441 Phosphorylation
Y442 Phosphorylation
K448 Ubiquitination
Y463 Phosphorylation
S467 Phosphorylation
T482 Phosphorylation
K485 Ubiquitination
S486 Phosphorylation P49841 (GSK3B) , O75385 (ULK1) , Q13131 (PRKAA1) , P17612 (PRKACA)
T488 Phosphorylation O75385 (ULK1)
T490 Phosphorylation P49841 (GSK3B)
S494 Phosphorylation Q13131 (PRKAA1)
S496 Phosphorylation Q13131 (PRKAA1) , P31749 (AKT1)
S498 Phosphorylation
Y500 Phosphorylation
S506 Phosphorylation
S508 Phosphorylation
K515 Ubiquitination
S520 Phosphorylation
T522 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation
T526 Phosphorylation
S527 Phosphorylation
S531 Phosphorylation
Site PTM Type Enzyme
K41 Ubiquitination
K45 Ubiquitination
K69 Acetylation
K69 Ubiquitination
K141 Ubiquitination
S161 Phosphorylation
S165 Phosphorylation
T172 Phosphorylation Q96RR4 (CAMKK2) , O43318 (MAP3K7) , Q15831 (STK11)
S173 Phosphorylation P17612 (PRKACA)
C174 S-Nitrosylation
S176 Phosphorylation
Y179 Phosphorylation
R227 Methylation
Y232 Phosphorylation
K260 Ubiquitination
K269 Ubiquitination
Y324 Phosphorylation
S377 Phosphorylation
K391 Ubiquitination
K401 Sumoylation
K401 Ubiquitination
Y436 Phosphorylation
S481 Phosphorylation
S483 Phosphorylation
T485 Phosphorylation Q15831 (STK11)
S489 Phosphorylation
S491 Phosphorylation P31749 (AKT1)
S500 Phosphorylation
S501 Phosphorylation
S509 Phosphorylation
S511 Phosphorylation
S515 Phosphorylation
T521 Phosphorylation
T524 Phosphorylation
S527 Phosphorylation
S534 Phosphorylation
Q13131/P54646 as PTM Enzyme
Substrate Site Source
O00418 (EEF2K) S78 HPRD
O00418 (EEF2K) S366 HPRD
O00418 (EEF2K) S398 Signor
O00429 (DNM1L) S637 Calculation
O00763 (ACACB) S222 Calculation
O15350 (TP73) S426 Calculation
O15360 (FANCA) S347 Calculation
O43524 (FOXO3) T179 Signor
O43524 (FOXO3) S399 Signor
O43524 (FOXO3) S413 Signor
O43524 (FOXO3) S555 Signor
O43524 (FOXO3) S588 Signor
O43524 (FOXO3) S626 Signor
O60825 (PFKFB2) S466 Signor
O75385 (ULK1) S317 neXtProt
O75385 (ULK1) S638 Calculation
O95278 (EPM2A) S25 Calculation
O95863 (SNAI1) S11 Signor
O95863 (SNAI1) S92 Signor
P00533 (EGFR) T892 Calculation
P04049 (RAF1) S259 Signor
P04049 (RAF1) S621 HPRD, neXtProt, Signor
P04406 (GAPDH) S122 Calculation
P04626 (ERBB2) T900 Calculation
P04637 (TP53) S15 Signor
P04637 (TP53) T18 Calculation
P04637 (TP53) S20 Calculation
P06400 (RB1) S811 Signor
P10636-8 (MAPT) S214 Calculation
P10636-8 (MAPT) T231 Calculation
P10636 (MAPT) S255 neXtProt
P10636-8 (MAPT) S262 Calculation
P10636 (MAPT) S355 neXtProt
P10636-8 (MAPT) S356 Calculation
P10636 (MAPT) S396 Calculation
P10636-8 (MAPT) S422 Calculation
P13569 (CFTR) S737 Calculation
P13569 (CFTR) S768 Calculation
P13569 (CFTR) S813 Calculation
P14859 (POU2F1) S335 Calculation
P14859 (POU2F1) S385 Signor
P15056 (BRAF) S729 Calculation
P15531 (NME1) S122 Calculation
P15531 (NME1) S144 Calculation
P17600 (SYN1) S9 Signor
P19429 (TNNI3) S23 Calculation
P19429 (TNNI3) S24 Calculation
P19429 (TNNI3) S150 Calculation
P29474 (NOS3) T495 Signor
P29474 (NOS3) S633 Calculation
P29474 (NOS3) S1177 Signor
P36956-3 (SREBF1) S372 Calculation
P41235 (HNF4A) S303 Signor
P41235 (HNF4A) S313 Calculation
P42345 (MTOR) T2446 HPRD
P43405 (SYK) S178 Calculation
P46527 (CDKN1B) T198 Calculation
P46937 (YAP1) S61 Calculation
P46937 (YAP1) S94 Calculation
P49116 (NR2C2) S351 Signor
P49674 (CSNK1E) S389 Calculation
P50552 (VASP) T278 Calculation
P52292 (KPNA2) S105 Signor
P54840 (GYS2) S8 neXtProt
P55011 (SLC12A2) S77 Calculation
P63244 (RACK1) T50 Calculation
Q04759 (PRKCQ) T538 neXtProt
Q05469 (LIPE) S855 Signor
Q06210 (GFPT1) S242 Signor
Q06210-2 (GFPT1) S243 Calculation
Q06210 (GFPT1) S261 Signor
Q07866 (KLC1) S521 Calculation
Q09472 (EP300) S89 Signor
Q12778 (FOXO1) T649 Calculation
Q13002-1 (GRIK2) S715 HPRD
Q13085-2 (ACACA) S22 HPRD
Q13085 (ACACA) S80 Calculation
Q13085-4 (ACACA) S117 HPRD
Q13085-1 (ACACA) S1201 HPRD
Q13085-4 (ACACA) S1238 HPRD
Q13131 (PRKAA1) T183 Calculation
Q13131 (PRKAA1) S360 Calculation
Q13131 (PRKAA1) T388 Calculation
Q13131 (PRKAA1) S486 Calculation
Q13131 (PRKAA1) S494 Calculation
Q13131 (PRKAA1) S496 Calculation
Q13362 (PPP2R5C) S298 Calculation
Q13362 (PPP2R5C) S336 Calculation
Q13363 (CTBP1) S158 Signor
Q13621 (SLC12A1) S122 neXtProt
Q13621 (SLC12A1) S130 Calculation
Q15036 (SNX17) S437 Calculation
Q16526 (CRY1) S71 Signor
Q16875 (PFKFB3) S461 Signor
Q53ET0 (CRTC2) S170 Signor
Q6N021 (TET2) S99 Signor
Q7Z3C6 (ATG9A) S761 Calculation
Q7Z628 (NET1) S100 Calculation
Q86TI0 (TBC1D1) S237 Signor
Q86TI0 (TBC1D1) T596 Calculation
Q8IXJ6 (SIRT2) T101 Calculation
Q8N122 (RPTOR) S722 Calculation
Q8N122 (RPTOR) S792 Calculation
Q8WUI4 (HDAC7) S155 Signor
Q8WUI4 (HDAC7) S358 Signor
Q92538 (GBF1) T1337 Signor
Q9BU19 (ZNF692) S470 Signor
Q9BZL4 (PPP1R12C) S452 Calculation
Q9GZY8 (MFF) S155 Signor
Q9GZY8 (MFF) S172 Signor
Q9H0B6 (KLC2) S539 neXtProt
Q9H0B6 (KLC2) S545 Calculation
Q9H0B6 (KLC2) S581 neXtProt
Q9H0B6 (KLC2) S582 Signor
Q9NYV6 (RRN3) S635 Signor
Q9P2M7 (CGN) S131 Calculation
Q9UBK2 (PPARGC1A) T178 Signor
Q9UQK1 (PPP1R3C) S33 Calculation
Q9UQK1 (PPP1R3C) S293 Calculation
Q9UQL6 (HDAC5) S259 Signor
Q9UQL6 (HDAC5) S498 Signor
Q9Y478 (PRKAB1) S24 Calculation
Q9Y478 (PRKAB1) T80 Calculation
Q9Y478 (PRKAB1) S108 Signor
Q9Y478 (PRKAB1) T148 Calculation
Q9Y478 (PRKAB1) T158 Calculation
Q9Y478 (PRKAB1) S174 Calculation
Q9Y478 (PRKAB1) S177 Calculation
Substrate Site Source
F1D8S2 (NR2A1) S313 HPRD
O00418 (EEF2K) S78 HPRD
O00418 (EEF2K) S366 HPRD
O00418 (EEF2K) S398 Signor
O00763-1 (ACACB) S222 HPRD, Signor
O14920 (IKBKB) S177 Signor
O14920 (IKBKB) S181 Signor
O15151 (MDM4) S342 Calculation
O43524 (FOXO3) T179 Calculation
O43524 (FOXO3) S399 Calculation
O43524 (FOXO3) S413 Calculation
O43524 (FOXO3) S555 Calculation
O43524 (FOXO3) S588 Calculation
O43524 (FOXO3) S626 Calculation
O60825 (PFKFB2) S466 HPRD, neXtProt, Signor
O75385 (ULK1) S317 Signor
O75385 (ULK1) S556 Signor
O75385 (ULK1) S638 Signor
P05549 (TFAP2A) S219 Calculation
P06241 (FYN) T12 neXtProt
P08151 (GLI1) S102 Calculation
P08151 (GLI1) S408 Calculation
P08151 (GLI1) T1074 Calculation
P09874 (PARP1) S177 Calculation
P28562 (DUSP1) S334 Calculation
P29474 (NOS3) S633 neXtProt
P29474 (NOS3) S1177 neXtProt
P30260 (CDC27) S379 Signor
P35222 (CTNNB1) S552 neXtProt
P36956 (SREBF1) S396 Signor
P41235-3 (HNF4A) S313 HPRD
P49815 (TSC2) S1387 Signor
P50552 (VASP) T278 Signor
P50552 (VASP) S322 Signor
P55011 (SLC12A2) S77 Calculation
P55011 (SLC12A2) S242 Calculation
Q13085-2 (ACACA) S22 HPRD
Q13085 (ACACA) S78 neXtProt
Q13085 (ACACA) S80 Signor
Q13085-4 (ACACA) S117 HPRD
Q13177 (PAK2) S20 Signor
Q13393 (PLD1) S505 Signor
Q15121 (PEA15) S116 Calculation
Q53ET0 (CRTC2) S171 Signor
Q86TI0 (TBC1D1) S237 neXtProt
Q8IY63 (AMOTL1) S793 Calculation
Q8N122 (RPTOR) S863 Signor
Q8NFG4 (FLCN) S62 Calculation
Q92819 (HAS2) T110 Calculation
Q96EB6 (SIRT1) T344 Calculation
Q9BZL4 (PPP1R12C) S452 Signor
Q9UQB8 (BAIAP2) S366 Signor
Q9UQL6 (HDAC5) S259 Calculation
Q9UQL6 (HDAC5) S498 Calculation
Q9Y2I7 (PIKFYVE) S307 Calculation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.