Phospho-NFAT4 (Ser165) Antibody - #AF3420

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Product: Phospho-NFAT4 (Ser165) Antibody
Catalog: AF3420
Description: Rabbit polyclonal antibody to Phospho-NFAT4 (Ser165)
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 120~150kD; 116kD(Calculated).
Uniprot: Q12968
RRID: AB_2834862

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-NFAT4 (Ser165) Antibody detects endogenous levels of NFAT4 only when phosphorylated at Serine 165.
RRID:
AB_2834862
Cite Format: Affinity Biosciences Cat# AF3420, RRID:AB_2834862.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C80703; cytoplasmic 3; D8Ertd281e; NF AT4; NF ATc3; NF-AT4; NF-ATc3; NFAC3_HUMAN; NFATc3; NFATx; Nuclear factor of activated T cells, cytoplasmic 3; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3; Nuclear factor of activated T-cells; T cell transcription factor NFAT4; T-cell transcription factor NFAT4;

Immunogens

Immunogen:
Uniprot:

Q12968(NFAC3_HUMAN) >>Visit HPA database.

Gene(ID):
NFATC3(4775)
Expression:
Q12968 NFAC3_HUMAN:

Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

Description:
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component.
Sequence:
MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSPCGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSDDQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETEGSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESKIIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPVPHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVVYNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVADQITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPSSLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDVNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12968 As Substrate

Site PTM Type Enzyme
S82 Phosphorylation
Y86 Phosphorylation
T89 Phosphorylation
K96 Ubiquitination
S98 Phosphorylation
S117 Phosphorylation
S145 Phosphorylation
Y150 Phosphorylation
S163 Phosphorylation P45984 (MAPK9)
S165 Phosphorylation P45984 (MAPK9)
S168 Phosphorylation
S169 Phosphorylation
S171 Phosphorylation
S172 Phosphorylation
S174 Phosphorylation
S177 Phosphorylation
S180 Phosphorylation
S181 Phosphorylation
S184 Phosphorylation
S186 Phosphorylation
T204 Phosphorylation P48729 (CSNK1A1)
S207 Phosphorylation P48729 (CSNK1A1)
T210 Phosphorylation P48729 (CSNK1A1)
S211 Phosphorylation P48729 (CSNK1A1)
S215 Phosphorylation P48729 (CSNK1A1)
S236 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
S247 Phosphorylation
S248 Phosphorylation
S261 Phosphorylation
S265 Phosphorylation
T268 Phosphorylation
S269 Phosphorylation
S277 Phosphorylation
S288 Phosphorylation
S292 Phosphorylation
S296 Phosphorylation
S300 Phosphorylation
K342 Ubiquitination
S344 Phosphorylation
K354 Ubiquitination
S364 Phosphorylation
S366 Phosphorylation
T371 Phosphorylation
S372 Phosphorylation
K384 Ubiquitination
K403 Ubiquitination
K405 Ubiquitination
S415 Phosphorylation
S416 Phosphorylation
K457 Ubiquitination
K467 Ubiquitination
K542 Ubiquitination
S546 Phosphorylation
K552 Ubiquitination
K630 Ubiquitination
K636 Ubiquitination
K649 Ubiquitination
S730 Phosphorylation
S731 Phosphorylation
S742 Phosphorylation
Y884 Phosphorylation
S1045 Phosphorylation
S1048 Phosphorylation
S1063 Phosphorylation
S1066 Phosphorylation

Research Backgrounds

Function:

Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Along with NFATC4, involved in embryonic heart development (By similarity).

PTMs:

Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

Subunit Structure:

Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.

Family&Domains:

Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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