ERLN2 Antibody - #AF0011
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0011, RRID:AB_2845470.
Fold/Unfold
C8orf2; Chromosome 8 open reading frame 2; Endoplasmic reticulum lipid raft associated protein 2; Endoplasmic reticulum lipid raft-associated protein 2; ER lipid raft associated 2; ERLIN 2; Erlin-2; ERLIN2; ERLN2_HUMAN; HGNC:1356; MGC87072; NET32; SPFH 2; SPFH domain containing protein 2; SPFH domain family member 2; SPFH domain-containing protein 2; SPFH2; SPG18; Stomatin prohibitin flotillin HflC/K domain containing protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2;
Immunogens
- O94905 ERLN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQLGAVVAVASSFFCASLFSAVHKIEEGHIGVYYRGGALLTSTSGPGFHLMLPFITSYKSVQTTLQTDEVKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIELFDQIDENLKLALQQDLTSMAPGLVIQAVRVTKPNIPEAIRRNYELMESEKTKLLIAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLAREKAKADAECYTAMKIAEANKLKLTPEYLQLMKYKAIASNSKIYFGKDIPNMFMDSAGSVSKQFEGLADKLSFGLEDEPLETATKEN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O94905 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| N106 | N-Glycosylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| Y263 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| T334 | O-Glycosylation | Uniprot | |
| T336 | O-Glycosylation | Uniprot |
Research Backgrounds
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1. Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex. Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex.
Endoplasmic reticulum membrane>Single-pass type II membrane protein.
Note: Associated with lipid raft-like domains of the endoplasmic reticulum membrane.
Ubiquitous.
Forms a heteromeric complex with ERLIN1. In complex with ERLIN1, interacts with RNF170. Interacts with activated ITPR1, independently of the degree of ITPR1 polyubiquitination (By similarity). Interacts with SCAP, INSIG1, SREBF1 and SREBF2 under cholesterol sufficiency conditions; indicative for an association with the SCAP-SREBP-INSIG complex. Probably part of an AMFR/gp78 and INSIG1-containing ubiquitin ligase complex involved in ERAD of HMGCR. Interacts with TMUB1; TMUB1 bridges the association with AMFR. Interacts with SYVN1 and RNF139. Interacts with TMEM259 (By similarity).
Belongs to the band 7/mec-2 family.
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