CAPG Antibody - #AF0013

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Product: CAPG Antibody
Catalog: AF0013
Description: Rabbit polyclonal antibody to CAPG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 39-44kDa; 38kD(Calculated).
Uniprot: P40121
RRID: AB_2845466

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CAPG Antibody detects endogenous levels of total CAPG.
RRID:
AB_2845466
Cite Format: Affinity Biosciences Cat# AF0013, RRID:AB_2845466.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin capping protein GCAP39;Actin regulatory protein CAP G;Actin regulatory protein CAP-G;AFCP;Capg;CAPG_HUMAN;Capping actin protein gelsolin like;Capping protein (actin filament) gelsolin like;Capping protein gelsolin like;Epididymis secretory protein Li 66;gCap39;Gelsolin like capping protein;HEL S 66;Macrophage capping protein;Macrophage-capping protein;mbh1;MCP;Myc basic motif homolog 1;

Immunogens

Immunogen:
Uniprot:

P40121(CAPG_HUMAN) >>Visit HPA database.

Gene(ID):
CAPG(822)
Expression:
P40121 CAPG_HUMAN:

Macrophages and macrophage-like cells.

Sequence:
MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGHESPIFKQFFKDWK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40121 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y2 Phosphorylation
T3 Phosphorylation
S8 Phosphorylation
S10 Phosphorylation
S103 Phosphorylation
S108 Phosphorylation
K115 Ubiquitination
S123 Phosphorylation
K127 Ubiquitination
T128 Phosphorylation
S129 Phosphorylation
K137 Acetylation
K137 Ubiquitination
K143 Ubiquitination
T151 Phosphorylation
K205 Ubiquitination
T212 Phosphorylation
K227 Ubiquitination
K231 Acetylation
K231 Ubiquitination
K243 Ubiquitination
K253 Ubiquitination
T258 Phosphorylation
K297 Ubiquitination
S318 Phosphorylation
Y322 Phosphorylation
S337 Phosphorylation

Research Backgrounds

Function:

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Nucleus. Cytoplasm. Melanosome. Cell projection>Lamellipodium. Cell projection>Ruffle.
Note: In macrophages, may be predominantly cytoplasmic. Nuclear localization was observed in fibroblasts. In macrophages, present at the membrane-cytoplasm interface. In activated macrophages, concentrated in the ruffles of the leading lamellipodia.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Macrophages and macrophage-like cells.

Subunit Structure:

Interacts with NUP62. Interacts with NUTF2 and RAN; involved in CAPG nuclear import.

Family&Domains:

Belongs to the villin/gelsolin family.

References

1). Proteome characteristics of liver tissue from patients with parenteral nutrition-associated liver disease. Nutrition & Metabolism, 2020 (PubMed: 32518576) [IF=4.5]

Application: WB    Species: human    Sample: Liver

Fig. 5| Confirmation of differentially expressed proteins (DEPs) by western blotting; a, c: Immunoblotting analysis of DEPs (CYP2B6, DDAH1,NDUFA1, CAPG, and FABP5) in PNALD(H-PN) and control (H-EN).

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