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  • Product Name
    Phospho-FAK (Tyr397) Antibody
  • Catalog No.
    AF3398
  • RRID
    AB_2834829
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
  • UniProt
  • Mol.Wt
    (Observed)119kD; (Calculated)119kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

FADK 1; FADK; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2;

Applications:

WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 397.

RRID:

AB_2834829
Please cite this product as: Affinity Biosciences Cat# AF3398, RRID:AB_2834829.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PTK2

Molecular Weight:

Observed Mol.Wt.: (Observed)119kD.
Predicted Mol.Wt.: (Calculated)119kDa.

Subcellular Location:

Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.

Tissue Specificity:

Q05397 FAK1_HUMAN:
Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Description:

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents.

Sequence:
MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH

Research Background

Function:

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Post-translational Modifications:

Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 (tyrosine phosphorylated). Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21. Phosphorylation on tyrosine residues is enhanced by NTN1 (By similarity).

Sumoylated; this enhances autophosphorylation.

Subcellular Location:

Cell junction>Focal adhesion. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus.
Note: Constituent of focal adhesions. Detected at microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Subunit Structure:

Interacts (via first Pro-rich region) with CAS family members (via SH3 domain), including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1. Interacts with SORBS1. Interacts with ARHGEF28. Interacts with SHB. Interacts with PXN and TLN1. Interacts with STAT1. Interacts with DCC. Interacts with WASL. Interacts with ARHGEF7. Interacts with GRB2 and GRB7 (By similarity). Component of a complex that contains at least FER, CTTN and PTK2/FAK1. Interacts with BMX. Interacts with TGFB1I1. Interacts with STEAP4. Interacts with ZFYVE21. Interacts with ESR1. Interacts with PIK3R1 or PIK3R2. Interacts with SRC, FGR, FLT4 and RET. Interacts with EPHA2 in resting cells; activation of EPHA2 recruits PTPN11, leading to dephosphorylation of PTK2/FAK1 and dissociation of the complex. Interacts with EPHA1 (kinase activity-dependent). Interacts with CD4; this interaction requires the presence of HIV-1 gp120. Interacts with PIAS1. Interacts with ARHGAP26 and SHC1. Interacts with RB1CC1; this inhibits PTK2/FAK1 activity and activation of downstream signaling pathways. Interacts with P53/TP53 and MDM2. Interacts with LPXN (via LD motif 3). Interacts with MISP. Interacts with CIB1 isoform 2. Interacts with CD36. Interacts with EMP2; regulates PTK2 activation and localization (PubMed:19494199). Interacts with DSCAM (By similarity).

Similarity:

The Pro-rich regions interact with the SH3 domain of CAS family members, such as BCAR1 and NEDD9, and with the GTPase activating protein ARHGAP26.

The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Small cell lung cancer.(View pathway)
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Organismal Systems > Immune system > Leukocyte transendothelial migration.(View pathway)
· Organismal Systems > Development > Axon guidance.(View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)

WB Images(1)


IHC Images(26)


IF/ICC Images(1)


ELISA analysis of AF3398 showing specificity to Phospho-FAK (Tyr397) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Fan Z et al. A tropomyosin-like Meretrix meretrix Linnaeus polypeptide inhibits the proliferation and metastasis of glioma cells via microtubule polymerization and FAK/Akt/MMPs signaling. Int J Biol Macromol 2019 Dec 20;145:154-164 (PubMed: 31866539) [IF=4.784]

2). Huang YX et al. Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells. PLoS One 2018 Apr 23;13(4):e0195610 (PubMed: 29684087)

3). Wu D et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget 2017 Aug 16;8(51):88630-88644 (PubMed: 29179463)

4). Wu D et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget 2017 Aug 16;8(51):88630-88644 (PubMed: 29179463)

5). Zhang W et al. Down-regulating Myoferlin inhibits the vasculogenic mimicry of melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition. J Cell Mol Med 2018 Mar;22(3):1743-1754 (PubMed: 29164766)

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Catalog Number :

AF3398-BP
(Blocking peptide available as AF3398-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Xenopus
100%
Chicken
100%
Rabbit
100%
Pig
100%
Dog
100%
Sheep
100%
Horse
100%
Zebrafish
0%
Bovine
0%
High similarity Medium similarity Low similarity No similarity
Q05397 as Substrate
Site PTM Type Enzyme
A2 Acetylation
Y5 Phosphorylation P09619 (PDGFRB) , P08581 (MET) , P00533 (EGFR)
T13 Phosphorylation
S16 Phosphorylation
T20 Phosphorylation
T24 Phosphorylation
S29 Phosphorylation
R65 Methylation
K70 Ubiquitination
Y148 Phosphorylation
Y155 Phosphorylation
Y194 Phosphorylation P09619 (PDGFRB) , P12931 (SRC) , P00533 (EGFR) , P51813 (BMX) , P08581 (MET)
K199 Ubiquitination
K216 Ubiquitination
S248 Phosphorylation
Y347 Phosphorylation
K364 Ubiquitination
S372 Phosphorylation
T386 Phosphorylation
S390 Phosphorylation
S392 Phosphorylation
T394 Phosphorylation
Y397 Phosphorylation P12931 (SRC) , Q05397 (PTK2) , P08581 (MET) , P46108 (CRK) , A0A059VC25 (FAK) , P09769 (FGR)
T406 Phosphorylation
Y407 Phosphorylation P08581 (MET) , Q05397 (PTK2) , Q14289 (PTK2B) , P12931 (SRC)
T408 Phosphorylation
Y441 Phosphorylation P51813 (BMX) , P12931 (SRC)
S443 Phosphorylation
T460 Phosphorylation
S461 Phosphorylation
S463 Phosphorylation
K467 Acetylation
T474 Phosphorylation
T503 Phosphorylation
S517 Phosphorylation
S568 Phosphorylation
Y570 Phosphorylation
S574 Phosphorylation
T575 Phosphorylation
Y576 Phosphorylation P08581 (MET) , P06241 (FYN) , P07949 (RET) , Q05397 (PTK2) , A0A059VC25 (FAK) , P51813 (BMX) , P07947 (YES1) , P09769 (FGR) , P12931 (SRC)
Y577 Phosphorylation P12931 (SRC) , A0A059VC25 (FAK) , P51813 (BMX) , P07949 (RET) , P08581 (MET) , Q05397 (PTK2)
S580 Phosphorylation
S677 Phosphorylation
K684 Ubiquitination
S695 Phosphorylation P31749 (AKT1)
T700 Phosphorylation P31749 (AKT1)
S702 Phosphorylation
S705 Phosphorylation
S708 Phosphorylation
S716 Phosphorylation
Y720 Phosphorylation
S722 Phosphorylation P49840 (GSK3A)
S726 Phosphorylation
S732 Phosphorylation Q13464 (ROCK1) , Q00535 (CDK5)
Y742 Phosphorylation P12931 (SRC) , P51813 (BMX)
S759 Phosphorylation
Y761 Phosphorylation
T805 Phosphorylation
S840 Phosphorylation
S843 Phosphorylation Q9UQM7 (CAMK2A)
S850 Phosphorylation
Y861 Phosphorylation Q05397 (PTK2) , P07949 (RET) , P08581 (MET) , Q13882 (PTK6) , P16591 (FER) , P12931 (SRC) , P51813 (BMX) , P07947 (YES1)
S887 Phosphorylation
S892 Phosphorylation
S893 Phosphorylation
Y898 Phosphorylation P51813 (BMX) , P12931 (SRC)
S910 Phosphorylation Q00535 (CDK5) , P27361 (MAPK3) , P28482 (MAPK1)
T914 Phosphorylation
S920 Phosphorylation
K923 Ubiquitination
Y925 Phosphorylation P12931 (SRC) , P16591 (FER) , P09769 (FGR) , P51813 (BMX) , P07947 (YES1) , P07949 (RET) , P08581 (MET)
T929 Phosphorylation
T963 Phosphorylation
T967 Phosphorylation
T971 Phosphorylation
K1000 Ubiquitination
Y1007 Phosphorylation P12931 (SRC) , P51813 (BMX)
K1017 Acetylation
Q05397 as PTM Enzyme
Substrate Site Source
O00401 (WASL) Y256 Uniprot
O43707 (ACTN4) Y4 Uniprot
O43707 (ACTN4) Y31 Uniprot
O43707 (ACTN4) Y265 Uniprot
O75962-1 (TRIO) Y2796 Uniprot
P07949 (RET) Y905 Uniprot
P12814-1 (ACTN1) Y12 Uniprot
P23634-6 (ATP2B4) Y1176 Uniprot
P26010 (ITGB7) Y753 Uniprot
P26010 (ITGB7) Y758 Uniprot
P29353 (SHC1) Y315 Uniprot
P49023 (PXN) Y31 Uniprot
P49023 (PXN) Y118 Uniprot
P51813 (BMX) Y40 Uniprot
P56945 (BCAR1) Y410 Uniprot
P56945-1 (BCAR1) Y664 Uniprot
P56945-1 (BCAR1) Y666 Uniprot
P63000 (RAC1) Y64 Uniprot
Q05397-1 (PTK2) S390 Uniprot
Q05397 (PTK2) Y397 Uniprot
Q05397 (PTK2) Y407 Uniprot
Q05397 (PTK2) Y576 Uniprot
Q05397 (PTK2) Y577 Uniprot
Q05397-1 (PTK2) S850 Uniprot
Q05397 (PTK2) Y861 Uniprot
Q05397-1 (PTK2) Y925 Uniprot
Q14451 (GRB7) Y188 Uniprot
Q14451 (GRB7) Y338 Uniprot
Q658W2 (DKFZp666O0110) S412 Uniprot
Q658W2 (DKFZp666O0110) Y419 Uniprot
Q658W2 (DKFZp666O0110) S872 Uniprot
Q658W2 (DKFZp666O0110) Y947 Uniprot
Q99961 (SH3GL1) Y315 Uniprot
Q9H5V8 (CDCP1) Y734 Uniprot
Q9H9S0 (NANOG) Y35 Uniprot
Q9H9S0 (NANOG) Y174 Uniprot
Q9Y2X7 (GIT1) Y321 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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