Phospho-TLK1 (Ser743) Antibody - #AF2425
| Product: | Phospho-TLK1 (Ser743) Antibody |
| Catalog: | AF2425 |
| Description: | Rabbit polyclonal antibody to Phospho-TLK1 (Ser743) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 86kDa; 87kD(Calculated). |
| Uniprot: | Q9UKI8 |
| RRID: | AB_2845438 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF2425, RRID:AB_2845438.
Fold/Unfold
PKU beta; PKU-beta; serine threonine protein kinase; Serine/threonine protein kinase tousled like 1; Serine/threonine-protein kinase tousled-like 1; SNARE protein kinase SNAK; Tlk1; TLK1_HUMAN; Tousled like kinase 1; Tousled-like kinase 1;
Immunogens
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
- Q9UKI8 TLK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UKI8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S22 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| T688 | Phosphorylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S744 | Phosphorylation | Uniprot | |
| S760 | Phosphorylation | Uniprot |
PTMs - Q9UKI8 As Enzyme
Research Backgrounds
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
Nucleus.
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.