Phospho-NEDD4L (Ser448) Antibody - #AF2370

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Product: Phospho-NEDD4L (Ser448) Antibody
Catalog: AF2370
Description: Rabbit polyclonal antibody to Phospho-NEDD4L (Ser448)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110, 135kDa; 112kD(Calculated).
Uniprot: Q96PU5
RRID: AB_2845384

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-NEDD4L (Ser448) Antibody detects endogenous levels of NEDD4L only when phosphorylated at Ser448.
RRID:
AB_2845384
Cite Format: Affinity Biosciences Cat# AF2370, RRID:AB_2845384.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; Nedd4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed, developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5;

Immunogens

Immunogen:
Uniprot:

Q96PU5(NED4L_HUMAN) >>Visit HPA database.

Gene(ID):
NEDD4L(23327)
Expression:
Q96PU5 NED4L_HUMAN:

Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).

Sequence:
MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96PU5 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S13 Phosphorylation
K25 Ubiquitination
S28 Phosphorylation
K129 Ubiquitination
K149 Ubiquitination
K155 Ubiquitination
S225 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
T302 Phosphorylation
S303 Phosphorylation
S312 Phosphorylation
T318 Phosphorylation
S321 Phosphorylation
S327 Phosphorylation
S328 Phosphorylation
S335 Phosphorylation
S336 Phosphorylation
S340 Phosphorylation
S342 Phosphorylation P17612 (PRKACA) , O00141 (SGK1)
T344 Phosphorylation
S357 Phosphorylation
S365 Phosphorylation
S366 Phosphorylation
T367 Phosphorylation P17612 (PRKACA) , O00141 (SGK1)
T369 Phosphorylation
T375 Phosphorylation
S377 Phosphorylation
Y380 Phosphorylation
T383 Phosphorylation
S428 Phosphorylation
S446 Phosphorylation
S448 Phosphorylation P17612 (PRKACA) , O00141 (SGK1) , Q96BR1 (SGK3)
S449 Phosphorylation
T451 Phosphorylation
T453 Phosphorylation
K462 Ubiquitination
S464 Phosphorylation
K471 Ubiquitination
T473 Phosphorylation
S475 Phosphorylation
S479 Phosphorylation
S483 Phosphorylation
Y485 Phosphorylation
S487 Phosphorylation
K489 Ubiquitination
K493 Ubiquitination
K521 Ubiquitination
K531 Ubiquitination
S538 Phosphorylation
K539 Ubiquitination
S571 Phosphorylation
K572 Ubiquitination
K639 Ubiquitination
K656 Ubiquitination
Y660 Phosphorylation
S795 Phosphorylation
T903 Phosphorylation

Research Backgrounds

Function:

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF.

PTMs:

Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.

Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.

Subcellular Location:

Cytoplasm. Golgi apparatus. Endosome>Multivesicular body.
Note: May be recruited to exosomes by NDFIP1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.

Subunit Structure:

Interacts with UBE2E3 (By similarity). Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with TNK2. Interacts with WNK1. Interacts with SGK1. Interacts (via C2 domain) with NPC2. Interacts with ARRDC4. Interacts with KCNQ1; promotes internalization of KCNQ1. Interacts (via domains WW1, 3 and 4) with USP36; the interaction inhibits ubiquitination of, at least, NTRK1, KCNQ2 and KCNQ3 by NEDD4L.

(Microbial infection) Interacts with Epstein-Barr virus LMP2A.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

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