Phospho-MFF (Ser172/Ser146) Antibody - #AF2365
Product: | Phospho-MFF (Ser172/Ser146) Antibody |
Catalog: | AF2365 |
Description: | Rabbit polyclonal antibody to Phospho-MFF (Ser172/Ser146) |
Application: | WB IHC |
Reactivity: | Human |
Prediction: | Rabbit |
Mol.Wt.: | 25, 27kDa; 38kD(Calculated). |
Uniprot: | Q9GZY8 |
RRID: | AB_2845379 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF2365, RRID:AB_2845379.
Fold/Unfold
C2orf33; Chromosome 2 open reading frame 33; DKFZp666J168; GL004; Mff; MFF_HUMAN; MGC110913; Mitochondrial fission factor; OTTHUMP00000164235;
Immunogens
- Q9GZY8 MFF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKGTSSDTSLGRVSRAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLWHRSDSAPRNKISRFQAPISAPEYTVTPSPQQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9GZY8 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S21 | Phosphorylation | Uniprot | |
Y35 | Phosphorylation | Uniprot | |
Y39 | Phosphorylation | Uniprot | |
T40 | Phosphorylation | Uniprot | |
K54 | Ubiquitination | Uniprot | |
S74 | Phosphorylation | Uniprot | |
S93 | Phosphorylation | Uniprot | |
T106 | Phosphorylation | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K114 | Ubiquitination | Uniprot | |
T115 | Phosphorylation | Uniprot | |
T121 | Phosphorylation | Uniprot | |
S123 | Phosphorylation | Uniprot | |
T137 | Phosphorylation | Uniprot | |
T138 | Phosphorylation | Uniprot | |
S155 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
S157 | Phosphorylation | Uniprot | |
S172 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
S177 | Phosphorylation | Uniprot | |
S179 | Phosphorylation | Uniprot | |
T200 | Phosphorylation | Uniprot | |
S202 | Phosphorylation | Uniprot | |
S222 | Phosphorylation | Uniprot | |
S223 | Phosphorylation | Uniprot | |
S229 | Phosphorylation | Uniprot | |
S233 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
S263 | Phosphorylation | Uniprot | |
K302 | Ubiquitination | Uniprot |
Research Backgrounds
Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.
Mitochondrion outer membrane>Single-pass type IV membrane protein. Peroxisome. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.
Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Homodimer. Interacts with DNM1L (By similarity).
Belongs to the Tango11 family.
References
Application: WB Species: Human Sample: A375P cells
Application: WB Species: Mouse Sample:
Application: WB Species: Rat Sample:
Application: WB Species: Mouse Sample:
Application: WB Species: Mouse Sample: M0-BV2 and M1-BV2 cells
Application: WB Species: Human Sample: A375P cells
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