Phospho-MCM3 (Ser112) Antibody | Affinity Biosciences

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AF2362 at 1/100 staining Mouse lung tissue by IHC-P.

Product:	Phospho-MCM3 (Ser112) Antibody
Catalog:	AF2362
Description:	Rabbit polyclonal antibody to Phospho-MCM3 (Ser112)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	100kDa; 91kD(Calculated).
Uniprot:	P25205
RRID:	AB_2845376

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-MCM3 (Ser112) Antibody detects endogenous levels of MCM3 only when phosphorylated at Ser112.

RRID:

AB_2845376
Cite Format: Affinity Biosciences Cat# AF2362, RRID:AB_2845376.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; mcm3; MCM3 minichromosome maintenance deficient 3; MCM3_HUMAN; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1-MCM3; P1.h; p102; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB;

Immunogens

Immunogen:

Uniprot:

P25205(MCM3_HUMAN) >>Visit HPA database.

Gene(ID):

MCM3(4172)

Sequence:

P25205 MCM3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25205 As Substrate

P25205

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
T4	Phosphorylation		Uniprot
Y19	Phosphorylation		Uniprot
Y32	Phosphorylation		Uniprot
S34	Phosphorylation		Uniprot
K35	Ubiquitination		Uniprot
Y45	Phosphorylation		Uniprot
R55	Methylation		Uniprot
K81	Ubiquitination		Uniprot
S112	Phosphorylation	P06493 (CDK1)	Uniprot
K140	Ubiquitination		Uniprot
S144	Phosphorylation		Uniprot
C148	S-Nitrosylation		Uniprot
K152	Acetylation		Uniprot
K152	Ubiquitination		Uniprot
T154	Phosphorylation		Uniprot
Y159	Phosphorylation		Uniprot
S160	Phosphorylation	P53355 (DAPK1) , O14757 (CHEK1)	Uniprot
T163	Phosphorylation		Uniprot
T164	Phosphorylation		Uniprot
S170	Phosphorylation		Uniprot
S171	Phosphorylation		Uniprot
T176	Phosphorylation		Uniprot
K177	Ubiquitination		Uniprot
T198	Phosphorylation		Uniprot
K207	Ubiquitination		Uniprot
K228	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
R234	Methylation		Uniprot
T240	Phosphorylation		Uniprot
K248	Sumoylation		Uniprot
K248	Ubiquitination		Uniprot
T252	Phosphorylation		Uniprot
T255	Phosphorylation		Uniprot
T258	Phosphorylation		Uniprot
C263	S-Nitrosylation		Uniprot
K266	Sumoylation		Uniprot
K266	Ubiquitination		Uniprot
K270	Ubiquitination		Uniprot
S275	Phosphorylation		Uniprot
K283	Ubiquitination		Uniprot
S292	Phosphorylation		Uniprot
K293	Ubiquitination		Uniprot
K301	Ubiquitination		Uniprot
Y312	Phosphorylation		Uniprot
K314	Ubiquitination		Uniprot
K315	Ubiquitination		Uniprot
K351	Ubiquitination		Uniprot
R371	Methylation		Uniprot
T383	Phosphorylation		Uniprot
T384	Phosphorylation		Uniprot
K413	Ubiquitination		Uniprot
R419	Methylation		Uniprot
K435	Ubiquitination		Uniprot
K463	Ubiquitination		Uniprot
S474	Phosphorylation		Uniprot
S535	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
Y545	Phosphorylation		Uniprot
T555	Phosphorylation		Uniprot
K556	Acetylation		Uniprot
K556	Ubiquitination		Uniprot
K559	Acetylation		Uniprot
K561	Ubiquitination		Uniprot
K570	Ubiquitination		Uniprot
K579	Ubiquitination		Uniprot
S599	Phosphorylation		Uniprot
S611	Phosphorylation		Uniprot
T617	Phosphorylation		Uniprot
K635	Ubiquitination		Uniprot
Y651	Phosphorylation		Uniprot
K655	Ubiquitination		Uniprot
S668	Phosphorylation		Uniprot
S672	Phosphorylation		Uniprot
T674	Phosphorylation		Uniprot
S681	Phosphorylation		Uniprot
K688	Ubiquitination		Uniprot
S704	Phosphorylation		Uniprot
Y705	Phosphorylation		Uniprot
Y708	Phosphorylation		Uniprot
S711	Phosphorylation		Uniprot
T713	Phosphorylation		Uniprot
T722	Phosphorylation	P24941 (CDK2)	Uniprot
T725	Phosphorylation		Uniprot
S728	Phosphorylation	Q13315 (ATM)	Uniprot
K732	Sumoylation		Uniprot
K732	Ubiquitination		Uniprot
S734	Phosphorylation		Uniprot
K736	Sumoylation		Uniprot
K736	Ubiquitination		Uniprot
S740	Phosphorylation		Uniprot
S742	Phosphorylation		Uniprot
R743	Methylation		Uniprot
K748	Ubiquitination		Uniprot

Research Backgrounds

P25205_MCM3_HUMAN

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

PTMs:

Acetylated by MCM3AP.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. Interacts with ANKRD17. Interacts with MCM3AP isoform MCM3AP; this interaction leads to MCM3 acetylation.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

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Phospho-MCM3 (Ser112) Antibody - #AF2362