Phospho-eEF2k (Ser366) Antibody - #AF2340
| Product: | Phospho-eEF2k (Ser366) Antibody |
| Catalog: | AF2340 |
| Description: | Rabbit polyclonal antibody to Phospho-eEF2k (Ser366) |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 105kDa; 82kD(Calculated). |
| Uniprot: | O00418 |
| RRID: | AB_2845354 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF2340, RRID:AB_2845354.
Fold/Unfold
C86191; Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; EC 2.7.11.20; eEF 2 kinase; eEF 2K; eEF-2 kinase; eEF-2K; Eef2k; EEF2K protein; EF2K_HUMAN; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; fa04b08; HSU93850; kinase eEF2K; MGC45041; SMEF2K; wu:fa04b08; zgc:100973;
Immunogens
- O00418 EF2K_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00418 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| Y37 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| K65 | Ubiquitination | Uniprot | |
| S66 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| Y69 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S74 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S78 | Phosphorylation | O00418 (EEF2K) , P54646 (PRKAA2) , Q13131 (PRKAA1) , Q96QT4 (TRPM7) | Uniprot |
| K82 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| R178 | Methylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| T348 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| T353 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| S359 | Phosphorylation | P06493 (CDK1) , O15264 (MAPK13) | Uniprot |
| T364 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Q15418 (RPS6KA1) , Q13131 (PRKAA1) , P17612 (PRKACA) , P54646 (PRKAA2) , O00418 (EEF2K) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2) | Uniprot |
| S377 | Phosphorylation | Q16644 (MAPKAPK3) , P49137 (MAPKAPK2) , Q8IW41 (MAPKAPK5) | Uniprot |
| S380 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | P45984 (MAPK9) , P45983 (MAPK8) , P53778 (MAPK12) , Q15759 (MAPK11) , O15264 (MAPK13) , O00418 (EEF2K) , Q16539 (MAPK14) | Uniprot |
| S398 | Phosphorylation | Q13131 (PRKAA1) , P54646 (PRKAA2) | Uniprot |
| S399 | Phosphorylation | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| Y443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| K447 | Ubiquitination | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| K467 | Sumoylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| S477 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S491 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| S492 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | O00418 (EEF2K) , P17612 (PRKACA) | Uniprot |
| K516 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| Y600 | Phosphorylation | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| K684 | Ubiquitination | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot |
PTMs - O00418 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S61 | Uniprot |
| O00418 (EEF2K) | S66 | Uniprot |
| O00418 (EEF2K) | S78 | Uniprot |
| O00418 (EEF2K) | T348 | Uniprot |
| O00418 (EEF2K) | T353 | Uniprot |
| O00418 (EEF2K) | S366 | Uniprot |
| O00418 (EEF2K) | S396 | Uniprot |
| O00418 (EEF2K) | S441 | Uniprot |
| O00418 (EEF2K) | S445 | Uniprot |
| O00418 (EEF2K) | S474 | Uniprot |
| O00418 (EEF2K) | S491 | Uniprot |
| O00418 (EEF2K) | S500 | Uniprot |
| P13639 (EEF2) | T54 | Uniprot |
| P13639 (EEF2) | T57 | Uniprot |
| P13639 (EEF2) | T59 | Uniprot |
Research Backgrounds
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
Monomer or homodimer (Probable). Interacts with Calmodulin/CALM1; this interaction is strictly required for phosphorylation activity.
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Research Fields
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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