Phospho-Dab1 (Tyr220) Antibody - #AF2335

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Product: Phospho-Dab1 (Tyr220) Antibody
Catalog: AF2335
Description: Rabbit polyclonal antibody to Phospho-Dab1 (Tyr220)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 110 kDa; 64kD(Calculated).
Uniprot: O75553
RRID: AB_2845349

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
Phospho-Dab1 (Tyr220) Antibody detects endogenous levels of Dab1 only when phosphorylated at Tyr220.
RRID:
AB_2845349
Cite Format: Affinity Biosciences Cat# AF2335, RRID:AB_2845349.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dab 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari;

Immunogens

Immunogen:
Uniprot:

O75553(DAB1_HUMAN) >>Visit HPA database.

Gene(ID):
DAB1(1600)
Expression:
O75553 DAB1_HUMAN:

Mainly expressed in brain.

Sequence:
MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Horse
92
Sheep
92
Xenopus
82
Pig
73
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75553 As Substrate

Site PTM Type Enzyme
K43 Ubiquitination
K45 Ubiquitination
K84 Sumoylation
Y198 Phosphorylation
Y220 Phosphorylation
Y232 Phosphorylation
S433 Phosphorylation
T506 Phosphorylation
S524 Phosphorylation P24941 (CDK2)
S526 Phosphorylation
S548 Phosphorylation

Research Backgrounds

Function:

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

PTMs:

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

Tissue Specificity:

Mainly expressed in brain.

Subunit Structure:

Associates with the SH2 domains of SRC, FYN and ABL (By similarity). Interacts (phosphorylated on tyrosine residues) with CRK and CRKL (via respective SH2 domain) (By similarity). Interacts with SIAH1, LRP8 and VLDLR (By similarity). Interacts with LRP1. Interacts with APLP1 (via NPXY motif) (By similarity). Interacts with DAB2IP (By similarity).

Family&Domains:

The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.

References

1). Rnf-213 Knockout Induces Pericyte Reduction and Blood-Brain Barrier Impairment in Mouse. Molecular Neurobiology (PubMed: 37438553) [IF=5.1]

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