CRAT Antibody | Affinity Biosciences

WB
IF/ICC

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Western blot analysis of extracts from mouse skeletal muscle tissue, using CRAT antibody.

Western blot analysis of extracts from Mouse muscle, using CRAT Antibody.

DF12372 staining Hela by IF/ICC.

DF12372 staining Hela cells by IF/ICC.

Product:	CRAT Antibody
Catalog:	DF12372
Description:	Rabbit polyclonal antibody to CRAT
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	55-68 kDa; 71kD(Calculated).
Uniprot:	P43155
RRID:	AB_2845177

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

CRAT Antibody detects endogenous levels of total CRAT.

RRID:

AB_2845177
Cite Format: Affinity Biosciences Cat# DF12372, RRID:AB_2845177.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CACP_HUMAN; Carnitine acetylase; Carnitine acetyltransferase; Carnitine O acetyltransferase; Carnitine O-acetyltransferase; CAT; CAT1; CrAT;

Immunogens

Immunogen:

Uniprot:

P43155(CACP_HUMAN) >>Visit HPA database.

Gene(ID):

CRAT(1384)

Expression:

P43155 CACP_HUMAN:

Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney.

Sequence:

P43155 CACP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLAFAARTVVKPLGFLKPFSLMKASSRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQASGGVGERLQKGLERRARKTENWLSEWWLKTAYLQYRQPVVIYSSPGVMLPKQDFVDLQGQLRFAAKLIEGVLDFKVMIDNETLPVEYLGGKPLCMNQYYQILSSCRVPGPKQDTVSNFSKTKKPPTHITVVHNYQFFELDVYHSDGTPLTADQIFVQLEKIWNSSLQTNKEPVGILTSNHRNSWAKAYNTLIKDKVNRDSVRSIQKSIFTVCLDATMPRVSEDVYRSHVAGQMLHGGGSRLNSGNRWFDKTLQFIVAEDGSCGLVYEHAAAEGPPIVTLLDYVIEYTKKPELVRSPLVPLPMPKKLRFNITPEIKSDIEKAKQNLSIMIQDLDITVMVFHHFGKDFPKSEKLSPDAFIQMALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASMDSLTFVKAMDDSSVTEHQKVELLRKAVQAHRGYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFHLSTSQVPAKTDCVMFFGPVVPDGYGVCYNPMEAHINFSLSAYNSCAETNAARLAHYLEKALLDMRALLQSHPRAKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Bovine

Sheep

Dog

Chicken

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43155 As Substrate

P43155

Site	PTM Type	Source
K29	Methylation	Uniprot
S59	Phosphorylation	Uniprot
Y107	Phosphorylation	Uniprot
Y110	Phosphorylation	Uniprot
Y117	Phosphorylation	Uniprot
K186	Acetylation	Uniprot
K195	Acetylation	Uniprot
T201	Phosphorylation	Uniprot
K261	Acetylation	Uniprot
K268	Acetylation	Uniprot
K379	Acetylation	Uniprot
K380	Ubiquitination	Uniprot
T467	Phosphorylation	Uniprot
S472	Phosphorylation	Uniprot
S485	Phosphorylation	Uniprot
Y606	Phosphorylation	Uniprot
K609	Acetylation	Uniprot

Research Backgrounds

P43155_CACP_HUMAN

Function:

Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. May be specific for short chain fatty acids.

Subcellular Location:

Endoplasmic reticulum. Peroxisome. Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Mitochondrion.

Peroxisome.

Tissue Specificity:

Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CRAT Antibody - #DF12372