Product: Peroxiredoxin 5 Antibody
Catalog: DF12307
Description: Rabbit polyclonal antibody to Peroxiredoxin 5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Xenopus
Mol.Wt.: 17 kDa; 22kD(Calculated).
Uniprot: P30044
RRID: AB_2845112

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(90%), Bovine(91%), Horse(91%), Sheep(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
Peroxiredoxin 5 Antibody detects endogenous levels of total Peroxiredoxin 5.
RRID:
AB_2845112
Cite Format: Affinity Biosciences Cat# DF12307, RRID:AB_2845112.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACR1; Alu co repressor 1; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; B166; epididymis secretory protein Li 55; HEL-S-55; Liver tissue 2D-page spot 71B; mitochondrial; Peroxiredoxin 5, mitochondrial; Peroxiredoxin V; Peroxiredoxin-5; Peroxisomal antioxidant enzyme; PLP; PMP20; PRDX 5; PRDX5; PRDX5_HUMAN; PRDX6; Prx-V; PRXV; SBBI10; Thioredoxin peroxidase PMP20; Thioredoxin reductase; TPx type VI;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P30044 PRDX5_HUMAN:

Widely expressed.

Sequence:
MGLAGVCALRRSAGYILVGGAGGQSAAAAARRYSEGEWASGGVRSFSRAAAAMAPIKVGDAIPAVEVFEGEPGNKVNLAELFKGKKGVLFGVPGAFTPGCSKTHLPGFVEQAEALKAKGVQVVACLSVNDAFVTGEWGRAHKAEGKVRLLADPTGAFGKETDLLLDDSLVSIFGNRRLKRFSMVVQDGIVKALNVEPDGTGLTCSLAPNIISQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Zebrafish
90
Xenopus
82
Dog
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30044 As Substrate

Site PTM Type Enzyme
S34 Phosphorylation
K75 Ubiquitination
K83 Acetylation
K83 Ubiquitination
K86 Ubiquitination
T97 Phosphorylation
C100 S-Nitrosylation
S101 Phosphorylation
K102 Ubiquitination
K116 Ubiquitination
K118 Ubiquitination
K146 Ubiquitination
K159 Ubiquitination
S182 Phosphorylation

Research Backgrounds

Function:

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

Subcellular Location:

Mitochondrion.

Cytoplasm. Peroxisome matrix.
Note: Imported into peroxisomes via peroxisomal targeting signal 1 receptor PEX5.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the peroxiredoxin family. Prx5 subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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