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$280 100ul
$350 200ul

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  • Product Name
    Phospho-Histone H3 (Ser11) Antibody
  • Catalog No.
    AF3358
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat, Spodoptera frugiperda
  • Prediction
    Bovine(100%)
  • UniProt
  • Mol.Wt.
    17kDa,16kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

H3 histone family, member A; H3/A; H31_HUMAN; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; Histone cluster 1, H3a; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; ;H3.3A; HIST1 cluster, H3E; H3 histone family, member A; H3.1; H3/l; H3F3; H3FF; H3FJ; H3FL; Histone gene cluster 1, H3 histone family, member E; histone H3.1t; Histone H3/o; FLJ92264; H 3; H3; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member T; H3 histone, family 3A; H3/A; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/t; H31_HUMAN; H3F1K; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FH; H3FI; H3FK; HIST1 cluster, H3A; HIST1 cluster, H3B; HIST1 cluster, H3C; HIST1 cluster, H3D; HIST1 cluster, H3F; HIST1 cluster, H3G; HIST1 cluster, H3H; HIST1 cluster, H3I; HIST1 cluster, H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1, H3a; Histone 1, H3b; Histone 1, H3c; Histone 1, H3d; Histone 1, H3e; Histone 1, H3f; Histone 1, H3g; Histone 1, H3h; Histone 1, H3i; Histone 3, H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1, H3a; Histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3d; Histone cluster 1, H3e; Histone cluster 1, H3f; Histone cluster 1, H3g; Histone cluster 1, H3i; Histone cluster 1, H3j; Histone gene cluster 1, H3 histone family, member A; Histone gene cluster 1, H3 histone family, member B; Histone gene cluster 1, H3 histone family, member C; Histone gene cluster 1, H3 histone family, member D; Histone gene cluster 1, H3 histone family, member F; Histone gene cluster 1, H3 histone family, member G; Histone gene cluster 1, H3 histone family, member H; Histone gene cluster 1, H3 histone family, member I; Histone gene cluster 1, H3 histone family, member J; Histone gene cluster 1, H3A; Histone gene cluster 1, H3B; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3E; Histone gene cluster 1, H3F; Histone gene cluster 1, H3G; Histone gene cluster 1, H3H; Histone gene cluster 1, H3I; Histone gene cluster 1, H3J; Histone H 3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; H3 histone family 3A; H3 histone family 3B; H3 histone, family 3B (H3.3B); H3.3; H3.3A; H3.3B; H33_HUMAN; H3F3; H3F3A; H3f3b; Histone H3.3; Histone H3.3Q; Histone H3.A; Histone H3.B; MGC87782; MGC87783;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat, Spodoptera frugiperda

Predicted Reactivity:

Bovine(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-Histone H3 (Ser11) Antibody detects endogenous levels of Histone H3 only when phosphorylated at Serine 11.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Histone H3 around the phosphorylation site of Ser11.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

P68431

Molecular Weight:

Observed Mol.Wt.: 17kDa.
Predicted Mol.Wt.: 16kDa.

Subcellular Location:

Nucleus. Chromosome.

Tissue Specificity:

Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

Description:

H3F3A Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis.

Sequence:
        10         20         30         40         50
MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR
60 70 80 90 100
EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY
110 120 130
LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA

Background

Function:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Post-translational Modifications:

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711). It gives a specific tag for epigenetic transcription activation (PubMed:29211711).

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Similarity:

Belongs to the histone H3 family.

Research Fields

Research Fields:

· Human Diseases > Immune diseases > Systemic lupus erythematosus.
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

Reference Citations:

1). Chi C et al. PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing. Apoptosis 2018 Aug 29 (PubMed: 30159848) [IF=4.021]

2). Cai NN et al. Schisandrin A and B enhance the dentate gyrus neurogenesis in mouse hippocampus. J Chem Neuroanat 2020 Feb 3;105:101751 (PubMed: 32027950)

3). Wu D et al. MAD2-p31comet axis deficiency reduces cell proliferation, migration and sensitivity of microtubule-interfering agents in glioma. Biochem Biophys Res Commun 2018 Mar 25;498(1):157-163 (PubMed: 29408509)

4). Liang X et al. Rictor regulates the vasculogenic mimicry of melanoma via the AKT-MMP-2/9 pathway. J Cell Mol Med 2017 Dec;21(12):3579-3591 (PubMed: 28699701)

Application: WB    Species:human;    Sample:Not available

Fig. 3 Knockdown of Rictor inhibits melanoma cells proliferation and blocked the cell cycle in G2/M phase. (A) Cell viability of A375 and MUM-2B cells after Rictor knockdown evaluated by MTT assay (*P < 0.05, **P < 0.01). (B) Cell cycle of A375 and MUM-2B cells after Rictor knockdown examined by FCM. (C) The expression of p-CDK2 and p-Histone H3 induced by knockdown of Rictor.


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Catalog Number :

AF3358-BP
(Blocking peptide available as AF3358-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Bovine
100%
Zebrafish
0%
Chicken
0%
Xenopus
0%
Rabbit
0%
Dog
0%
Horse
0%
Sheep
0%
Pig
0%
High similarity Medium similarity Low similarity No similarity
P68431/Q71DI3/P84243 as Substrate
Site PTM Type Enzyme
M1 Acetylation
R3 Methylation
T4 Phosphorylation Q99986 (VRK1)
K5 Acetylation
K5 Methylation
T7 Phosphorylation
K10 Acetylation
K10 Methylation
S11 Phosphorylation P31751 (AKT2) , Q99986 (VRK1) , Q96KB5 (PBK) , Q13153 (PAK1) , Q96GD4 (AURKB) , O14965 (AURKA) , Q16539 (MAPK14) , P51812 (RPS6KA3) , P11309 (PIM1) , P21980 (TGM2) , Q9UQB9 (AURKC) , O75582 (RPS6KA5)
T12 Phosphorylation O14757 (CHEK1) , O43293 (DAPK3)
K15 Acetylation
R18 Methylation
K19 Acetylation
K19 Methylation
K24 Acetylation
K24 Methylation
K28 Acetylation
K28 Methylation
S29 Phosphorylation P27361 (MAPK3) , P31751 (AKT2) , Q96GD4 (AURKB) , P45984 (MAPK9) , O75582 (RPS6KA5) , P28482 (MAPK1) , Q16539 (MAPK14) , P45983 (MAPK8)
K37 Acetylation
K37 Methylation
K37 Ubiquitination
K38 Methylation
Y42 Phosphorylation
T46 Phosphorylation Q05655 (PRKCD) , P31751 (AKT2)
K57 Acetylation
K57 Methylation
S58 Phosphorylation
K65 Methylation
K80 Acetylation
K80 Methylation
T81 Phosphorylation
S87 Phosphorylation
C97 S-Nitrosylation
K116 Acetylation
K123 Acetylation
K123 Methylation
Site PTM Type Enzyme
M1 Acetylation
R3 Methylation
T4 Phosphorylation
K5 Acetylation
K5 Methylation
T7 Phosphorylation
R9 Methylation
K10 Acetylation
K10 Methylation
S11 Phosphorylation
T12 Phosphorylation
K15 Acetylation
K15 Sumoylation
K15 Ubiquitination
R18 Methylation
K19 Acetylation
K19 Methylation
K19 Sumoylation
K19 Ubiquitination
K24 Acetylation
K24 Methylation
K24 Sumoylation
K24 Ubiquitination
K28 Acetylation
K28 Methylation
K28 Ubiquitination
S29 Phosphorylation
K37 Acetylation
K37 Methylation
K37 Ubiquitination
K38 Methylation
R41 Methylation
Y42 Phosphorylation
R43 Methylation
T46 Phosphorylation
R50 Methylation
K57 Acetylation
K57 Methylation
K57 Sumoylation
K57 Ubiquitination
S58 Phosphorylation
T59 Phosphorylation
R64 Methylation
K65 Methylation
K80 Acetylation
K80 Methylation
K80 Sumoylation
K80 Ubiquitination
T81 Phosphorylation
R84 Methylation
S97 Phosphorylation
Y100 Phosphorylation
T108 Phosphorylation
K116 Acetylation
K116 Ubiquitination
T119 Phosphorylation
K123 Acetylation
K123 Methylation
K123 Sumoylation
K123 Ubiquitination
R129 Methylation
Site PTM Type Enzyme
M1 Acetylation
R3 Methylation
T4 Phosphorylation
K5 Acetylation
K5 Methylation
T7 Phosphorylation
K10 Acetylation
K10 Methylation
S11 Phosphorylation P51812 (RPS6KA3) , O75582 (RPS6KA5) , O15111 (CHUK) , P06241 (FYN) , P31749 (AKT1) , Q15418 (RPS6KA1) , Q96GD4 (AURKB) , P17612 (PRKACA)
T12 Phosphorylation O14757 (CHEK1)
K15 Acetylation
R18 Methylation
K19 Acetylation
K19 Methylation
K24 Acetylation
K24 Methylation
K28 Acetylation
K28 Methylation
S29 Phosphorylation P17612 (PRKACA) , P28482 (MAPK1) , P27361 (MAPK3) , O75582 (RPS6KA5) , P45983 (MAPK8) , P45984 (MAPK9)
S32 Phosphorylation
K37 Acetylation
K37 Methylation
K37 Ubiquitination
Y42 Phosphorylation O60674 (JAK2)
T46 Phosphorylation
K57 Acetylation
K57 Methylation
S58 Phosphorylation
K65 Methylation
K80 Acetylation
K80 Methylation
T81 Phosphorylation
S87 Phosphorylation
C111 S-Nitrosylation
K116 Acetylation
K123 Acetylation
K123 Methylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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