CPT1A Antibody - #DF12004

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Product: CPT1A Antibody
Catalog: DF12004
Description: Rabbit polyclonal antibody to CPT1A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Dog
Mol.Wt.: 86 kDa, 70 kDa; 88kD(Calculated).
Uniprot: P50416
RRID: AB_2844809

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(85%), Dog(92%)
Clonality:
Polyclonal
Specificity:
CPT1A Antibody detects endogenous levels of total CPT1A.
RRID:
AB_2844809
Cite Format: Affinity Biosciences Cat# DF12004, RRID:AB_2844809.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Carnitine O palmitoyltransferase 1 liver isoform; Carnitine O palmitoyltransferase I; Carnitine O palmitoyltransferase I liver isoform; Carnitine O-palmitoyltransferase 1; Carnitine O-palmitoyltransferase I; Carnitine palmitoyltransferase 1A (liver); Carnitine palmitoyltransferase 1A; Carnitine palmitoyltransferase I; Carnitine palmitoyltransferase I liver; CPT 1; CPT I; CPT1; CPT1 L; CPT1-L; Cpt1a; CPT1A_HUMAN; CPTI; CPTI-L; L CPT1; liver isoform;

Immunogens

Immunogen:
Uniprot:

P50416(CPT1A_HUMAN) >>Visit HPA database.

Gene(ID):
CPT1A(1374)
Expression:
P50416 CPT1A_HUMAN:

Strong expression in kidney and heart, and lower in liver and skeletal muscle.

Sequence:
MAEAHQAVAFQFTVTPDGIDLRLSHEALRQIYLSGLHSWKKKFIRFKNGIITGVYPASPSSWLIVVVGVMTTMYAKIDPSLGIIAKINRTLETANCMSSQTKNVVSGVLFGTGLWVALIVTMRYSLKVLLSYHGWMFTEHGKMSRATKIWMGMVKIFSGRKPMLYSFQTSLPRLPVPAVKDTVNRYLQSVRPLMKEEDFKRMTALAQDFAVGLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYYAMDLLYILPTHIQAARAGNAIHAILLYRRKLDREEIKPIRLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHMRDSKHIVVYHRGRYFKVWLYHDGRLLKPREMEQQMQRILDNTSEPQPGEARLAALTAGDRVPWARCRQAYFGRGKNKQSLDAVEKAAFFVTLDETEEGYRSEDPDTSMDSYAKSLLHGRCYDRWFDKSFTFVVFKNGKMGLNAEHSWADAPIVAHLWEYVMSIDSLQLGYAEDGHCKGDINPNIPYPTRLQWDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLINFHISSKFSCPETDSHRFGRHLKEAMTDIITLFGLSSNSKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Bovine
85
Pig
77
Sheep
77
Horse
69
Xenopus
69
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50416 As Substrate

Site PTM Type Enzyme
K86 Ubiquitination
S106 Phosphorylation
S158 Phosphorylation
K161 Ubiquitination
Y165 Phosphorylation
K180 Ubiquitination
K195 Ubiquitination
K200 Ubiquitination
K292 Ubiquitination
T322 Phosphorylation
S371 Phosphorylation
K405 Ubiquitination
K441 Ubiquitination
Y514 Phosphorylation
K634 Ubiquitination
S671 Phosphorylation
K675 Ubiquitination
K755 Ubiquitination
S771 Phosphorylation
K772 Ubiquitination

Research Backgrounds

Function:

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism.

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strong expression in kidney and heart, and lower in liver and skeletal muscle.

Subunit Structure:

Homohexamer and homotrimer. Identified in a complex that contains at least CPT1A, ACSL1 and VDAC1. Also identified in complexes with ACSL1 and VDAC2 and VDAC3 (By similarity).

Family&Domains:

A conformation change in the N-terminal region spanning the first 42 residues plays an important role in the regulation of enzyme activity by malonyl-CoA.

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Dihydroartemisinin is potential therapeutics for treating late-stage CRC by targeting the elevated c-Myc level. Cell Death & Disease (PubMed: 34741022) [IF=9.0]

Application: WB    Species: Mouse    Sample: xenograft tissues

Fig. 4 Dihydroartemisinin reduces c-Myc transcriptional activity, and its downstream targets involved in lipid metabolism. A c-Myc reporter activity in DLD-1 and HCT116 cells after dihydroartemisinin (DHA, 10 µM) treatment. The data are shown as means ± SEM, n = 3 independent experiments, *p 
2). β-patchoulene improves lipid metabolism to alleviate non-alcoholic fatty liver disease via activating AMPK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY (PubMed: 33341045) [IF=7.5]

Application: WB    Species: Human    Sample: L02 cell

Fig. 6. β-PAE promotes the expression of hepatic lipid oxidation-related proteins and genes in HFD-fed rats. (A–G) Western blot analysis on the expression of SIRT1, PGC-1α, PPARα, FGF21, CPT-1a and ACOX1; (H–K) The mRNA expression of SIRT1, PPARα, CPT-1a and ACOX1. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.
3). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.
4). Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (PubMed: 36695046) [IF=6.1]
5). Roxadustat, a Hypoxia-Inducible Factor 1α Activator, Attenuates Both Long-and Short-Term Alcohol-Induced Alcoholic Liver Disease. Frontiers in Pharmacology (PubMed: 35620283) [IF=5.6]

Application: WB    Species: Human    Sample: HepG2 cells

FIGURE 2 Roxadustat attenuates lipid accumulation by increasing PPARα protein expression in the liver and reducing FASN levels in HepG2 cells. (A–D) Liver samples were collected from mice in Figure 1 and used for the following experiments. Liver frozen sections were stained with Oil Red O staining (A); triglyceride content was determined using an assay kit (B); mRNA expression of DGAT1 and FASN was determined by qRT-PCR (C); protein expression of PPARα and CPT1A was determined by Western blot with quantitative analysis of band density (D); (E) HepG2 cells were treated with roxadustat at indicated concentrations for 24 h. Protein expression of FASN and HIF-1α was determined by Western blot with quantitative analysis of band density (right panels); (F,G) HepG2 cells were transfected with scrambled siRNA (si-NC) or HIF-1α siRNA (si-HIF-1α) for 24 h, and then treated with roxadustat for 24 h. Protein expression of FASN, PPARα, and HIF-1α was determined by Western blot with quantitative analysis of band density (F); lipid accumulation was determined by Oil Red O staining with quantitative analysis (G). *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs ctrl; # p < 0.05, ###, p < 0.001 vs ALD group (n ≥ 5); Roxa: roxadustat.
6). Protective effects of Lactobacillus acidophilus NX2-6 against oleic acid-induced steatosis, mitochondrial dysfunction, endoplasmic reticulum stress and inflammatory responses. Journal of Functional Foods [IF=5.6]
7). Patchouli alcohol ameliorates acute liver injury via inhibiting oxidative stress and gut-origin LPS leakage in rats. International Immunopharmacology (PubMed: 34182243) [IF=5.6]
8). Selegiline ameliorated dyslipidemia and hepatic steatosis in high-fat diet mice. International Immunopharmacology (PubMed: 36822098) [IF=5.6]
9). Methyl Brevifolincarboxylate Attenuates Free Fatty Acid-Induced Lipid Metabolism and Inflammation in Hepatocytes through AMPK/NF-κB Signaling Pathway. International Journal of Molecular Sciences (PubMed: 34576229) [IF=5.6]
10). Caffeic acid dimethyl ether alleviates alcohol-induced hepatic steatosis via microRNA-378b-mediated CaMKK2-AMPK pathway. Bioengineered (PubMed: 35481488) [IF=4.9]

Application: WB    Species: mouse    Sample: livers

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice. (a,b) Western blot analysis of the protein expressions of SREBP-1c, FASN, PPARα, CPT1, MTTP and protein ratio of p-foxO1/foxO1 when the mice were injected with AAV-miR-378b agomir and antagomir. All data are expressed as the means ± SD of at least three separate experiments.
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