Rabbit Anti-Goat IgG (H+L) HRP - S0010

	Price	Size
	$280	100ul
	$350	200ul

Product Information

Applications:

WB: 1:5000~1:10000, IHC 1:200
*The optimal dilutions should be determined by the end user.

Reactivity:

Goat

Source:

Rabbit

Clonality:

Polyclonal

Purification:

affinity purification.

RRID:

AB_2844795
Please cite this product as: Affinity Biosciences Cat# S0010, RRID:AB_2844795.

Storage Condition and Buffer:

IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Immunogen Information

Immunogen:

The antiserum was developed in Rabbit using the Goat IgG as the immunogen.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Protocols & Troubleshooting

IHC Protocol.

Sample Preparation For Western Blotting.

Western Blotting Handbook.

Not available

Reference Citations:

1). Zhou WJ;Wang DD;Tao J;Tai Y;Zhou ZW;Wang Z;Guo PP;Sun WY;Chen JY;Wu HX;Yan SX;Zhang LL;Wang QT;Wei W; et al. Deficiency of β-arrestin2 exacerbates inflammatory arthritis by facilitating plasma cell formation. Acta Pharmacol Sin 2020 Aug 27. (PubMed: 32855529) [IF=5.064]

2). Zeng SY et al. Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells. Mediators Inflamm 2018 Jun 12;2018:2109352 (PubMed: 30046277) [IF=3.758]

3). Zhao ZH et al. PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury. Cell Mol Neurobiol 2017 May;37(4):707-715 (PubMed: 27443384) [IF=3.606]

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Catalog Number :

S0010-BP
(Blocking peptide available as S0010-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted secondary antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 37°C, 2H.

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